Volume 11 Issue 3
May  2021
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CHEN Yu, XU Yanan, PANG Yan. Advances in research on the occurrence, source and risk assessment of antibiotics[J]. Journal of Environmental Engineering Technology, 2021, 11(3): 562-570. doi: 10.12153/j.issn.1674-991X.20200180
Citation: CHEN Yu, XU Yanan, PANG Yan. Advances in research on the occurrence, source and risk assessment of antibiotics[J]. Journal of Environmental Engineering Technology, 2021, 11(3): 562-570. doi: 10.12153/j.issn.1674-991X.20200180

Advances in research on the occurrence, source and risk assessment of antibiotics

doi: 10.12153/j.issn.1674-991X.20200180
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  • Corresponding author: PANG Yan E-mail: 190068749@qq.com
  • Received Date: 2020-07-22
  • Publish Date: 2021-05-20
  • The current use of antibiotics at home and abroad, the pollution in the water environment, the main sources and the risk assessment methods of antibiotics were summarized based on a literature review of the studies on antibiotics by different scholars in the past ten years. The results showed that the types of antibiotics in the water environment mainly included quinolones, sulfonamides, macrolides and tetracyclines. Compared with foreign countries, the types of antibiotics detected in rivers and lakes in China were more abundant, and the pollution situation was more complicated. There were certain differences in the main pollution types of antibiotics in inland and coastal water. The main sources of antibiotics in the water environment were urban sewage plant effluent, medical wastewater, livestock and poultry breeding and aquaculture wastewater. At present, a corresponding evaluation index system had been established for single pollution sources and mixed pollution sources in water bodies and sediments, but the toxicological data, evaluation methods and influencing factors urgently needed to be improved. It was recommended to strengthen the source control of antibiotics, enhance the wastewater treatment plant process and improve the risk assessment system.

     

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  • [1]
    LIU X H, LU S Y, MENG W, et al. Occurrence,source,and ecological risk of antibiotics in Dongting Lake,China[J]. Environmental Science and Pollution Research, 2018, 25(11):11063-11073.
    doi: 10.1007/s11356-018-1290-1
    [2]
    XU Y, GUO C S, LUO Y, et al. Occurrence and distribution of antibiotics,antibiotic resistance genes in the urban rivers in Beijing,China[J]. Environmental Pollution, 2016, 213:833-840.
    doi: 10.1016/j.envpol.2016.03.054
    [3]
    KAFAEI R, PAPARI F, SEYEDABADI M, et al. Occurrence,distribution,and potential sources of antibiotics pollution in the water-sediment of the northern coastline of the Persian Gulf,Iran[J]. Science of the Total Environment, 2018, 627:703-712.
    doi: 10.1016/j.scitotenv.2018.01.305
    [4]
    van BOECKEL T P, BROWER C, GILBERT M, et al. Global trends in antimicrobial use in food animals[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(18):5649-5654.
    [5]
    CYCOŃM, MROZIK A, PIOTROWSKA-SEGET Z. Antibiotics in the soil environment: degradation and their impact on microbial activity and diversity[J]. Frontiers in Microbiology, 2019, 10:338.
    doi: 10.3389/fmicb.2019.00338
    [6]
    GONZÁLEZ-PLEITER M, CIRÉS S, HURTADO-GALLEGO J, et al. Ecotoxicological assessment of antibiotics in freshwater using cyanobacteria[M]// Cyanobacteria.Amsterdam: Elsevier, 2019:399-417.
    [7]
    YANG Y, OK Y S, KIM K H, et al. Occurrences and removal of pharmaceuticals and personal care products(PPCPs) in drinking water and water/sewage treatment plants:a review[J]. Science of the Total Environment, 2017, 596/597:303-320.
    doi: 10.1016/j.scitotenv.2017.04.102
    [8]
    莫苑敏, 黄亮亮, 王倩, 等. 广西青狮潭水库水体喹诺酮类抗生素的分布特征及生态风险评价[J]. 湖泊科学, 2019, 31(1):124-133.
    doi: 10.18307/2019.0112

    MO Y M, HUANG L L, WANG Q, et al. Distribution characteristics and ecological risk assessment of quinolone antibiotics in Qingshitan Reservoir,Guangxi Province[J]. Journal of Lake Sciences, 2019, 31(1):124-133. doi: 10.18307/2019.0112
    [9]
    BIELEN A, ŠIMATOVIĆ A, KOSIĆ-VUKŠIĆ J, et al. Negative environmental impacts of antibiotic-contaminated effluents from pharmaceutical industries[J]. Water Research, 2017, 126:79-87.
    doi: 10.1016/j.watres.2017.09.019
    [10]
    LIU X H, ZHANG H B, LI L Z, et al. Levels Levels, distributions and sources of veterinary antibiotics in the sediments of the Bohai Sea in China and surrounding estuaries[J]. Marine Pollution Bulletin, 2016, 109(1):597-602.
    doi: 10.1016/j.marpolbul.2016.05.033
    [11]
    LIU X, STEELE J C, MENG X Z. Usage,residue,and human health risk of antibiotics in Chinese aquaculture:a review[J]. Environmental Pollution, 2017, 223:161-169.
    doi: 10.1016/j.envpol.2017.01.003
    [12]
    SOUZA D M, REICHERT J F, MARTINS A F. A simultaneous determination of anti-cancer drugs in hospital effluent by DLLME HPLC-FLD,together with a risk assessment[J]. Chemosphere, 2018, 201:178-188.
    doi: 10.1016/j.chemosphere.2018.02.164
    [13]
    LI S, SHI W Z, LIU W, et al. A duodecennial national synthesis of antibiotics in China’s major rivers and seas(2005-2016)[J]. Science of the Total Environment,2018, 615:906-917.
    doi: 10.1016/j.scitotenv.2017.09.328
    [14]
    SHOKOOHI R, GHOBADI N, GODINI K, et al. Antibiotic detection in a hospital wastewater and comparison of their removal rate by activated sludge and earthworm-based vermifilteration:environmental risk assessment[J]. Process Safety and Environmental Protection, 2020, 134:169-177.
    doi: 10.1016/j.psep.2019.10.020
    [15]
    WANG S, MA X X, LIU Y L, et al. Fate of antibiotics,antibiotic-resistant bacteria,and cell-free antibiotic-resistant genes in full-scale membrane bioreactor wastewater treatment plants[J]. Bioresource Technology, 2020, 302:122825.
    doi: 10.1016/j.biortech.2020.122825
    [16]
    LI S J, JU H Y, ZHANG J Q, et al. Occurrence and distribution of selected antibiotics in the surface waters and ecological risk assessment based on the theory of natural disaster[J]. Environmental Science and Pollution Research, 2019, 26(27):28384-28400.
    doi: 10.1007/s11356-019-06060-7
    [17]
    ZHANG Q Q, YING G G, PAN C G, et al. Comprehensive evaluation of antibiotics emission and fate in the river basins of China:source analysis,multimedia modeling,and linkage to bacterial resistance[J]. Environmental Science & Technology, 2015, 49(11):6772-6782.
    doi: 10.1021/acs.est.5b00729
    [18]
    LI B, ZHANG T. Different removal behaviours of multiple trace antibiotics in municipal wastewater chlorination[J]. Water Research, 2013, 47(9):2970-2982.
    doi: 10.1016/j.watres.2013.03.001
    [19]
    CHEN H Y, JING L J, TENG Y G, et al. Characterization of antibiotics in a large-scale river system of China:occurrence pattern,spatiotemporal distribution and environmental risks[J]. Science of the Total Environment, 2018, 618:409-418.
    doi: 10.1016/j.scitotenv.2017.11.054
    [20]
    XU Z A, LI T, BI J, et al. Spatiotemporal heterogeneity of antibiotic pollution and ecological risk assessment in Taihu Lake Basin,China[J]. Science of the Total Environment, 2018, 643:12-20.
    doi: 10.1016/j.scitotenv.2018.06.175
    [21]
    TANG J, SHI T Z, WU X W, et al. The occurrence and distribution of antibiotics in Lake Chaohu,China:seasonal variation,potential source and risk assessment[J]. Chemosphere, 2015, 122:154-161.
    doi: 10.1016/j.chemosphere.2014.11.032
    [22]
    CHEN K, ZHOU J L. Occurrence and behavior of antibiotics in water and sediments from the Huangpu River,Shanghai,China[J]. Chemosphere, 2014, 95:604-612.
    doi: 10.1016/j.chemosphere.2013.09.119
    [23]
    MA Y P, LI M, WU M M, et al. Occurrences and regional distributions of 20 antibiotics in water bodies during groundwater recharge[J]. Science of the Total Environment, 2015, 518/519:498-506.
    doi: 10.1016/j.scitotenv.2015.02.100
    [24]
    LIANG X M, CHEN B W, NIE X P, et al. The distribution and partitioning of common antibiotics in water and sediment of the Pearl River Estuary,South China[J]. Chemosphere, 2013, 92(11):1410-1416.
    doi: 10.1016/j.chemosphere.2013.03.044
    [25]
    BU Q W, WANG B, HUANG J, et al. Pharmaceuticals and personal care products in the aquatic environment in China:a review[J]. Journal of Hazardous Materials, 2013, 262:189-211.
    doi: 10.1016/j.jhazmat.2013.08.040
    [26]
    DENG W J, LI N, ZHENG H L, et al. Occurrence and risk assessment of antibiotics in river water in Hong Kong[J]. Ecotoxicology and Environmental Safety, 2016, 125:121-127.
    doi: 10.1016/j.ecoenv.2015.12.002
    [27]
    KÜMMERER K. Antibiotics in the aquatic environment:a review:Part Ⅰ[J]. Chemosphere, 2009, 75(4):417-434.
    doi: 10.1016/j.chemosphere.2008.11.086
    [28]
    ZHANG G D, LU S Y, WANG Y Q, et al. Occurrence of antibiotics and antibiotic resistance genes and their correlations in Lower Yangtze River,China[J]. Environmental Pollution, 2020, 257:113365.
    doi: 10.1016/j.envpol.2019.113365
    [29]
    DING H J, WU Y X, ZHANG W H, et al. Occurrence,distribution,and risk assessment of antibiotics in the surface water of Poyang Lake,the largest freshwater lake in China[J]. Chemosphere, 2017, 184:137-147.
    doi: 10.1016/j.chemosphere.2017.05.148
    [30]
    CHEN H, LIU S, XU X R, et al. Antibiotics in the coastal environment of the Hailing Bay Region,South China Sea:spatial distribution,source analysis and ecological risks[J]. Marine Pollution Bulletin, 2015, 95(1):365-373.
    doi: 10.1016/j.marpolbul.2015.04.025
    [31]
    HU Y, YAN X, SHEN Y, et al. Antibiotics in surface water and sediments from Hanjiang River,Central China:occurrence,behavior and risk assessment[J]. Ecotoxicology and Environmental Safety, 2018, 157:150-158.
    doi: 10.1016/j.ecoenv.2018.03.083
    [32]
    LEI X N, LU J J, LIU Z L, et al. Concentration and distribution of antibiotics in water-sediment system of Bosten Lake,Xinjiang[J]. Environmental Science and Pollution Research, 2015, 22(3):1670-1678.
    doi: 10.1007/s11356-014-2994-5
    [33]
    TRAN N H, HOANG L, NGHIEM L D, et al. Occurrence and risk assessment of multiple classes of antibiotics in urban canals and lakes in Hanoi,Vietnam[J]. Science of the Total Environment, 2019, 692:157-174.
    doi: 10.1016/j.scitotenv.2019.07.092
    [34]
    AWAD Y M, KIM S C, EL-AZEEM S A M, et al. Veterinary antibiotics contamination in water,sediment,and soil near a swine manure composting facility[J]. Environmental Earth Sciences, 2014, 71(3):1433-1440.
    doi: 10.1007/s12665-013-2548-z
    [35]
    WATKINSON A J, MURBY E J, KOLPIN D W, et al. The occurrence of antibiotics in an urban watershed:from wastewater to drinking water[J]. Science of the Total Environment, 2009, 407(8):2711-2723.
    doi: 10.1016/j.scitotenv.2008.11.059
    [36]
    KLEYWEGT S, PILEGGI V, YANG P, et al. Pharmaceuticals Pharmaceuticals, hormones and bisphenol A in untreated source and finished drinking water in Ontario,Canada:occurrence and treatment efficiency[J]. Science of the Total Environment, 2011, 409(8):1481-1488.
    doi: 10.1016/j.scitotenv.2011.01.010
    [37]
    ZUCCATO E, CASTIGLIONI S, BAGNATI R, et al. Source, occurrence and fate of antibiotics in the Italian aquatic environment[J]. Journal of Hazardous Materials, 2010, 179(1/2/3):1042-1048.
    doi: 10.1016/j.jhazmat.2010.03.110
    [38]
    KHAN G A, BERGLUND B, KHAN K M, et al. Occurrence and abundance of antibiotics and resistance genes in rivers,canal and near drug formulation facilities:a study in Pakistan[J]. PLoS One, 2013, 8(6):e62712.
    doi: 10.1371/journal.pone.0062712
    [39]
    秦延文, 张雷, 时瑶, 等. 大辽河表层水体典型抗生素污染特征与生态风险评价[J]. 环境科学研究, 2015, 28(3):361-368.

    QIN Y W, ZHANG L, SHI Y, et al. Contamination characteristics and ecological risk assessment of typical antibiotics in surface water of the Daliao River,China[J]. Research of Environmental Sciences, 2015, 28(3):361-368.
    [40]
    EVGENIDOU E N, KONSTANTINOU I K, LAMBROPOULOU D A. Occurrence and removal of transformation products of PPCPs and illicit drugs in wastewaters:a review[J]. Science of the Total Environment, 2015, 505:905-926.
    doi: 10.1016/j.scitotenv.2014.10.021
    [41]
    ROWE W P M, BAKER-AUSTIN C, VERNER-JEFFREYS D W, et al. Overexpression of antibiotic resistance genes in hospital effluents over time[J]. Journal of Antimicrobial Chemotherapy, 2017, 72(6):1617-1623.
    doi: 10.1093/jac/dkx017
    [42]
    LI W H, SHI Y L, GAO L H, et al. Occurrence of antibiotics in water,sediments,aquatic plants,and animals from Baiyangdian Lake in North China[J]. Chemosphere, 2012, 89(11):1307-1315.
    doi: 10.1016/j.chemosphere.2012.05.079
    [43]
    HE K, SOARES A D, ADEJUMO H, et al. Detection of a wide variety of human and veterinary fluoroquinolone antibiotics in municipal wastewater and wastewater-impacted surface water[J]. Journal of Pharmaceutical and Biomedical Analysis, 2015, 106:136-143.
    doi: 10.1016/j.jpba.2014.11.020
    [44]
    DINH Q, MOREAU-GUIGON E, LABADIE P, et al. Fate of antibiotics from hospital and domestic sources in a sewage network[J]. Science of the Total Environment, 2017, 575:758-766.
    doi: 10.1016/j.scitotenv.2016.09.118
    [45]
    AYDIN S, AYDIN M E, ULVI A, et al. Antibiotics in hospital effluents:occurrence,contribution to urban wastewater,removal in a wastewater treatment plant,and environmental risk assessment[J]. Environmental Science and Pollution Research, 2019, 26(1):544-558.
    doi: 10.1007/s11356-018-3563-0
    [46]
    VERLICCHI P, AL-AUKIDY M, GALLETTI A, et al. Hospital effluent: investigation of the concentrations and distribution of pharmaceuticals and environmental risk assessment[J]. Science of the Total Environment, 2012, 430:109-118.
    doi: 10.1016/j.scitotenv.2012.04.055
    [47]
    BIROŠOVÁ L, MACKUL’AK T, BODÍK I, et al. Pilot study of seasonal occurrence and distribution of antibiotics and drug resistant bacteria in wastewater treatment plants in Slovakia[J]. Science of the Total Environment, 2014, 490:440-444.
    doi: 10.1016/j.scitotenv.2014.05.030
    [48]
    SAMARAWEERA D N D, LIU X, ZHONG G C, et al. Antibiotics in two municipal sewage treatment plants in Sri Lanka:occurrence,consumption and removal efficiency[J]. Emerging Contaminants, 2019, 5:272-278.
    doi: 10.1016/j.emcon.2019.08.001
    [49]
    SANTOS L H M L M, GROS M, RODRIGUEZ-MOZAZ S, et al. Contribution of hospital effluents to the load of pharmaceuticals in urban wastewaters:identification of ecologically relevant pharmaceuticals[J]. Science of the Total Environment, 2013, 461/462:302-316.
    doi: 10.1016/j.scitotenv.2013.04.077
    [50]
    WANG W H, ZHANG W F, LIANG H, et al. Occurrence and fate of typical antibiotics in wastewater treatment plants in Harbin,North-East China[J]. Frontiers of Environmental Science & Engineering, 2019, 13(3):1-10.
    [51]
    YUAN S F, LIU Z H, YIN H, et al. Trace determination of sulfonamide antibiotics and their acetylated metabolites via SPE-LC-MS/MS in wastewater and insights from their occurrence in a municipal wastewater treatment plant[J]. Science of the Total Environment, 2019, 653:815-821.
    doi: 10.1016/j.scitotenv.2018.10.417
    [52]
    ZHANG X, ZHAO H X, DU J, et al. Occurrence,removal,and risk assessment of antibiotics in 12 wastewater treatment plants from Dalian,China[J]. Environmental Science and Pollution Research, 2017, 24(19):16478-16487.
    doi: 10.1007/s11356-017-9296-7
    [53]
    WU M H, QUE C J, XU G, et al. Occurrence Occurrence, fate and interrelation of selected antibiotics in sewage treatment plants and their receiving surface water[J]. Ecotoxicology and Environmental Safety, 2016, 132:132-139.
    doi: 10.1016/j.ecoenv.2016.06.006
    [54]
    OLIVEIRA T S, MURPHY M, MENDOLA N, et al. Characterization of pharmaceuticals and personal care products in hospital effluent and waste water influent/effluent by direct-injection LC-MS-MS[J]. Science of the Total Environment, 2015, 518/519:459-478.
    doi: 10.1016/j.scitotenv.2015.02.104
    [55]
    BINH V N, DANG N, ANH N T K, et al. Antibiotics in the aquatic environment of Vietnam:sources,concentrations,risk and control strategy[J]. Chemosphere, 2018, 197:438-450.
    doi: 10.1016/j.chemosphere.2018.01.061
    [56]
    AZUMA T, OTOMO K, KUNITOU M, et al. Environmental fate of pharmaceutical compounds and antimicrobial-resistant bacteria in hospital effluents,and contributions to pollutant loads in the surface waters in Japan[J]. Science of the Total Environment, 2019, 657:476-484.
    doi: 10.1016/j.scitotenv.2018.11.433
    [57]
    MENDOZA A, ACEÑA J, PÉREZ S, et al. Pharmaceuticals and iodinated contrast media in a hospital wastewater:a case study to analyse their presence and characterise their environmental risk and hazard[J]. Environmental Research, 2015, 140:225-241.
    doi: 10.1016/j.envres.2015.04.003
    [58]
    WANG Q, WANG P L, YANG Q X. Occurrence and diversity of antibiotic resistance in untreated hospital wastewater[J]. Science of the Total Environment, 2018, 621:990-999.
    doi: 10.1016/j.scitotenv.2017.10.128
    [59]
    LIN A Y C, TSAI Y T. Occurrence of pharmaceuticals in Taiwan’s surface waters:impact of waste streams from hospitals and pharmaceutical production facilities[J]. Science of the Total Environment, 2009, 407(12):3793-3802.
    doi: 10.1016/j.scitotenv.2009.03.009
    [60]
    CHANG X S, MEYER M T, LIU X Y, et al. Determination of antibiotics in sewage from hospitals,nursery and slaughter house,wastewater treatment plant and source water in Chongqing Region of Three Gorge Reservoir in China[J]. Environmental Pollution, 2010, 158(5):1444-1450.
    doi: 10.1016/j.envpol.2009.12.034
    [61]
    LI C, LU J, LIU J, et al. Exploring the correlations between antibiotics and antibiotic resistance genes in the wastewater treatment plants of hospitals in Xinjiang,China[J]. Environmental Science and Pollution Research International, 2016, 23(15):15111-15121.
    doi: 10.1007/s11356-016-6688-z
    [62]
    MIRZAEI R, YUNESIAN M, NASSERI S, et al. Occurrence and fate of most prescribed antibiotics in different water environments of Tehran, Iran[J]. Science of the Total Environment, 2018, 619/620:446-459.
    doi: 10.1016/j.scitotenv.2017.07.272
    [63]
    何秀婷, 王奇, 聂湘平, 等. 广东典型海水养殖区沉积物及鱼体中磺胺类药物的残留及其对人体的健康风险评价[J]. 环境科学, 2014, 35(7):2728-2735.

    HE X T, WANG Q, NIE X P, et al. Residues and health risk assessment of sulfonamides in sediment and fish from typical marine aquaculture regions of Guangdong Province,China[J]. Environmental Science, 2014, 35(7):2728-2735.
    [64]
    中国海产网. 长江流域抗生素污染调查[EB/OL].( 2020-04-26)[ 2020-12-25]. http://www.haichaninfo.com/news/show-76771.html.
    [65]
    SIEDLEWICZ G, BIAŁK-BIELIŃSKA A, BORECKA M, et al. Presence Presence, concentrations and risk assessment ofselected antibiotic residues in sediments and near-bottom waters collected from the Polish coastal zone in the southern Baltic Sea:summary of 3 years of studies[J]. Marine Pollution Bulletin, 2018, 129(2):787-801.
    doi: 10.1016/j.marpolbul.2017.10.075
    [66]
    WANG Z, DU Y, YANG C, et al. Occurrence and ecological hazard assessment of selected antibiotics in the surface waters in and around Lake Honghu,China[J]. Science of the Total Environment, 2017, 609:1423-1432.
    doi: 10.1016/j.scitotenv.2017.08.009
    [67]
    VRYZAS Z, ALEXOUDIS C, VASSILIOU G, et al. Determination and aquatic risk assessment of pesticide residues in riparian drainage canals in Northeastern Greece[J]. Ecotoxicology and Environmental Safety, 2011, 74(2):174-181.
    doi: 10.1016/j.ecoenv.2010.04.011
    [68]
    LI J, MA L Y, LI L S, et al. Photodegradation kinetics,transformation,and toxicity prediction of ketoprofen,carprofen,and diclofenac acid in aqueous solutions[J]. Environmental Toxicology and Chemistry, 2017, 36(12):3232-3239.
    doi: 10.1002/etc.3915
    [69]
    章琴琴. 北京温榆河流域抗生素污染分布特征及源解析研究[D]. 重庆: 重庆大学, 2012.
    [70]
    DONG D M, ZHANG L W, LIU S, et al. Antibiotics in water and sediments from Liao River in Jilin Province,China:occurrence,distribution,and risk assessment[J]. Environmental Earth Sciences, 2016, 75(16):1-10.
    doi: 10.1007/s12665-015-4873-x
    [71]
    SHARMA B M, BEČANOVÁ J, SCHERINGER M, et al. Health and ecological risk assessment of emerging contaminants(pharmaceuticals,personal care products,and artificial sweeteners) in surface and groundwater(drinking water) in the Ganges River Basin,India[J]. Science of the Total Environment, 2019, 646:1459-1467.
    doi: 10.1016/j.scitotenv.2018.07.235
    [72]
    张盼伟, 周怀东, 赵高峰, 等. 太湖表层沉积物中PPCPs的时空分布特征及潜在风险[J]. 环境科学, 2016, 37(9):3348-3355.

    ZHANG P W, ZHOU H D, ZHAO G F, et al. Spatial Spatial, temporal distribution characteristics and potential risk of PPCPs in surface sediments from Taihu Lake[J]. Environmental Science, 2016, 37(9):3348-3355.
    [73]
    ZHOU L J, LI J, ZHANG Y D, et al. Trends in the occurrence and risk assessment of antibiotics in shallow lakes in the lower-middle reaches of the Yangtze River Basin,China[J]. Ecotoxicology and Environmental Safety, 2019, 183:109511.
    doi: 10.1016/j.ecoenv.2019.109511
    [74]
    LI F F, CHEN L J, CHEN W D, et al. Antibiotics in coastal water and sediments of the East China Sea:distribution,ecological risk assessment and indicators screening[J]. Marine Pollution Bulletin, 2020, 151:110810.
    doi: 10.1016/j.marpolbul.2019.110810
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