| Citation: | ZHANG J,ZHOU N,ZHANG P Y,et al.Application of immobilized microbial technology in polycyclic aromatic hydrocarbon-contaminated soil remediation[J].Journal of Environmental Engineering Technology，2024，14（5）：1617-1626 doi: 10.12153/j.issn.1674-991X.20240079
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Immobilized microbial technology is considered as an effective and environmentally friendly technology for the remediation of polycyclic aromatic hydrocarbons (PAHs)-contaminated soil. With this technology, microorganisms are protected from the surrounding harsh environment so that functional microorganisms remain active in PAHs-contaminated soil for a long time, overcoming the problems of low efficiency and long cycle of traditional microbial remediation. The current status of soil PAHs pollution in China and common bioremediation technologies were introduced. The current research status of immobilized microbial technology and the important factors affecting bacterial immobilization were summarized from carrier materials, PAHs-degrading bacteria and immobilization conditions. The remediation mechanisms of PAHs-contaminated soil with immobilized microbial technology were analyzed from soil environment, microbial community and adsorption-degradation process. Finally, the problems of immobilized microbial technology in the remediation of PAHs-contaminated soil were summarized and future research prospects were proposed from carrier materials, functional microorganisms, immobilization methods, evaluation systems, ecological risks and practical applications.
| [1] |
吴洁婷, 许琪, 张营, 等. 微生物降解典型高分子量多环芳烃的研究进展[J]. 环境科学研究,2021,34(8):1981-1990.
WU J T, XU Q, ZHANG Y, et al. Progress in microbial degradation of typical HMW-PAHs[J]. Research of Environment Sciences,2021,34(8):1981-1990.
|
| [2] |
李敏. 菌群QY1的降解特性及其联合生物炭高效降解多环芳烃研究[D]. 广州: 华南理工大学, 2021.
|
| [3] |
CHAKRAVARTY P, CHOWDHURY D, DEKA H M. Ecological risk assessment of priority PAHs pollutants in crude oil contaminated soil and its impacts on soil biological properties[J]. Journal of Hazardous Materials,2022,437:129325. doi: 10.1016/j.jhazmat.2022.129325
|
| [4] |
花洁, 王健媛, 陈运帷, 等. 煤矿矿区土壤重金属及多环芳烃污染治理修复技术综述[J]. 环境工程技术学报,2024,14(1):139-147. doi: 10.12153/j.issn.1674-991X.20230524
HUA J, WANG J Y, CHEN Y W, et al. A review of heavy metal and polycyclic aromatic hydrocarbon pollution treatment and remediation technologies in coal mine soils[J]. Journal of Environmental Engineering Technology,2024,14(1):139-147. doi: 10.12153/j.issn.1674-991X.20230524
|
| [5] |
李花, 赵立坤, 包仕钰, 等. 多环芳烃降解菌及其应用研究进展[J]. 环境工程技术学报,2023,13(5):1663-1676. doi: 10.12153/j.issn.1674-991X.20230152
LI H, ZHAO L K, BAO S Y, et al. Research progress on polycyclic aromatic hydrocarbons degrading bacteria and their applications[J]. Journal of Environmental Engineering Technology,2023,13(5):1663-1676. doi: 10.12153/j.issn.1674-991X.20230152
|
| [6] |
史哲明, 郭琳, 房桂明, 等. 微生物修复多环芳烃污染土壤的研究进展[J]. 现代化工,2022,42(10):24-28.
SHI Z M, GUO L, FANG G M, et al. Research progress on microbial remediation of polycyclic aromatic hydrocarbons contaminated soil[J]. Modern Chemical Industry,2022,42(10):24-28.
|
| [7] |
WOLF D C, CRYDER Z, KHOURY R, et al. Bioremediation of PAH-contaminated shooting range soil using integrated approaches[J]. Science of the Total Environment,2020,726:138440. doi: 10.1016/j.scitotenv.2020.138440
|
| [8] |
王建龙, 施汉昌, 钱易. 固定化微生物技术在难降解有机污染物治理中的研究进展[J]. 环境科学研究,1999,12(1):60-63. doi: 10.3321/j.issn:1001-6929.1999.01.016
WANG J L, SHI H C, QIAN Y. The advances in biodegradation of refractory originic pollutants by immobilized microbial cells[J]. Research of Environment Sciences,1999,12(1):60-63. doi: 10.3321/j.issn:1001-6929.1999.01.016
|
| [9] |
许盼, 李凯, 陈肖晓, 等. 固定化微生物降解PAHs的研究进展[J]. 应用技术学报,2021,21(4):348-355.
XU P, LI K, CHEN X X, et al. Research progress on degradation of PAHs by immobilized microorganisms[J]. Journal of Technology,2021,21(4):348-355.
|
| [10] |
朱晓丽, 张婵娟, 张星, 等. 生物炭固定化硫酸盐还原菌对镉污染土壤的钝化修复[J]. 环境科学学报,2023,43(5):421-429.
ZHU X L, ZHANG C J, ZHANG X, et al. Remediation of Cd2+ contaminated soil by biochar immobilized sulfate reducing bacteria[J]. Acta Scientiae Circumstantiae,2023,43(5):421-429.
|
| [11] |
付蓓. 水凝胶微球固定化芽孢杆菌BQ-3的制备及其对芘降解性能及途径研究[D]. 桂林: 桂林理工大学, 2023.
|
| [12] |
李琋, 王雅璇, 罗廷, 等. 利用生物炭负载微生物修复石油烃-镉复合污染土壤[J]. 环境工程学报,2021,15(2):677-687. doi: 10.12030/j.cjee.202003093
LI X, WANG Y X, LUO T, et al. Remediation of petroleum hydrocarbon-cadmium co-contaminated soil by biochar loaded microorganisms[J]. Chinese Journal of Environmental Engineering,2021,15(2):677-687. doi: 10.12030/j.cjee.202003093
|
| [13] |
李启虔. 基于真菌固定化技术的多环芳烃污染土壤的生物修复研究[D]. 北京: 中国科学院大学, 2021.
|
| [14] |
刘少恒. 重金属胁迫下固定化Bacillus sp. P1对多环芳烃的去除机理研究[D]. 长沙: 湖南大学, 2018.
|
| [15] |
张秀霞, 任文海, 丁明山, 等. 污泥生物炭固定化微生物强化石油污染土壤修复[J]. 石油学报(石油加工),2023,39(4):892-899.
ZHANG X X, REN W H, DING M S, et al. Enhanced remediation of petroleum contaminated soil by sludge biochar immobilized microorganisms[J]. Acta Petrolei Sinica (Petroleum Processing Section),2023,39(4):892-899.
|
| [16] |
张博凡. 菌糠及菌糠炭联合微生物修复石油污染土壤研究[D]. 东营: 中国石油大学(华东), 2019.
|
| [17] |
LIU J Y, LIU Y, DONG W H, et al. Shifts in microbial community structure and function in polycyclic aromatic hydrocarbon contaminated soils at petrochemical landfill sites revealed by metagenomics[J]. Chemosphere,2022,293:133509. doi: 10.1016/j.chemosphere.2021.133509
|
| [18] |
李娜, 刘睿, 台培东, 等. 植物-固定化菌剂联合修复多环芳烃污染土壤[J]. 应用生态学报,2021,32(8):2982-2988.
LI N, LIU R, TAI P D, et al. Combined remediation of polycyclic aromatic hydrocarbons (PAHs) by plant and immobilized bacteria in contaminated soil[J]. Chinese Journal of Applied Ecology,2021,32(8):2982-2988.
|
| [19] |
孙安琪. 炭基材料固定化微生物及其对PAHs污染土壤的修复研究[D]. 厦门: 华侨大学, 2019.
|
| [20] |
WANG S X, LI X J, LIU W, et al. Degradation of pyrene by immobilized microorganisms in saline-alkaline soil[J]. Journal of Environmental Sciences (China),2012,24(9):1662-1669. doi: 10.1016/S1001-0742(11)60963-7
|
| [21] |
李岩, 李成, 张小雪, 等. 番茄秸秆固定化芽孢杆菌M1对3环PAHs污染老化土壤修复效果[J]. 农业资源与环境学报,2019,36(6):806-813.
LI Y, LI C, ZHANG X X, et al. Remediation effects of 3-ring PAH-contaminated soil by immobilized Bacillus sp. M1 with tomato straw in coal mining area[J]. Journal of Agricultural Resources and Environment,2019,36(6):806-813.
|
| [22] |
于金标. 酒糟固定化微生物对PAHs污染土壤的修复作用[D]. 徐州: 中国矿业大学, 2023.
|
| [23] |
HAYES M H B. Biochar and biofuels for a brighter future[J]. Nature,2006,443(7108):144.
|
| [24] |
GUO S S, LIU X M, TANG J C. Enhanced degradation of petroleum hydrocarbons by immobilizing multiple bacteria on wheat bran biochar and its effect on greenhouse gas emission in saline-alkali soil[J]. Chemosphere, 2022, 286(Pt 2): 131663.
|
| [25] |
HUANG R Y, TIAN W J, LIU Q, et al. Enhanced biodegradation of pyrene and indeno(1,2,3-Cd)pyrene using bacteria immobilized in cinder beads in estuarine wetlands[J]. Marine Pollution Bulletin,2016,102(1):128-133. doi: 10.1016/j.marpolbul.2015.11.044
|
| [26] |
巩春娟, 苏丹, 王鑫, 等. 不同载体材料固定化耐低温混合菌修复PAHs污染土壤[J]. 生态学杂志,2018,37(12):3713-3720.
GONG C J, SU D, WANG X, et al. Impacts of cold-resistant mixed strains immobilized by different carrier materials on remediation of PAHs polluted soils[J]. Chinese Journal of Ecology,2018,37(12):3713-3720.
|
| [27] |
REN W J, LIU H R, MAO T Y, et al. Enhanced remediation of PAHs-contaminated site soil by bioaugmentation with graphene oxide immobilized bacterial pellets[J]. Journal of Hazardous Materials,2022,433:128793. doi: 10.1016/j.jhazmat.2022.128793
|
| [28] |
WANG K, SUN Y, CHEN D Y, et al. Enhanced remediation of phenanthrene in water and soil by novel biochar-immobilized bacterial microspheres[J]. Chemical Engineering Journal,2023,462:141932. doi: 10.1016/j.cej.2023.141932
|
| [29] |
滕庭庭, 梁继东. PAHs降解功能菌的识别与生物强化修复PAHs污染土壤研究进展[J]. 应用技术学报,2022,22(1):16-26. doi: 10.3969/j.issn.2096-3424.2022.01.003
TENG T T, LIANG J D. Research progress on identification of PAHs-degrading bacteria and bioremediation of PAHs-contaminated soil[J]. Journal of Technology,2022,22(1):16-26. doi: 10.3969/j.issn.2096-3424.2022.01.003
|
| [30] |
GHOSH I, JASMINE J, MUKHERJI S. Biodegradation of pyrene by a Pseudomonas aeruginosa strain RS1 isolated from refinery sludge[J]. Bioresource Technology,2014,166:548-558. doi: 10.1016/j.biortech.2014.05.074
|
| [31] |
王天杰. 丝瓜络及其固定化微生物对环境中PAHs的吸附-降解作用[D]. 沈阳: 辽宁大学, 2020.
|
| [32] |
任静, 沈佳敏, 张磊, 等. 生物炭固定化多环芳烃高效降解菌剂的制备及稳定性[J]. 环境科学学报,2020,40(12):4517-4523.
REN J, SHEN J M, ZHANG L, et al. Preparation and stability of biochar for the immobilization of polycyclic aromatic hydrocarbons degradating-bacteria[J]. Acta Scientiae Circumstantiae,2020,40(12):4517-4523.
|
| [33] |
胡广军, 梁成华, 李培军, 等. 固定化微生物对多环芳烃污染土壤的降解[J]. 生态学杂志,2008,27(5):745-750.
HU G J, LIANG C H, LI P J, et al. Degradation of soil polycyclic aromatic hydrocarbons by immobilized microbes[J]. Chinese Journal of Ecology,2008,27(5):745-750.
|
| [34] |
XIONG B J, ZHANG Y C, HOU Y W, et al. Enhanced biodegradation of PAHs in historically contaminated soil by M. gilvum inoculated biochar[J]. Chemosphere,2017,182:316-324. doi: 10.1016/j.chemosphere.2017.05.020
|
| [35] |
SONG L C, NIU X G, ZHANG N W, et al. Effect of biochar-immobilized Sphingomonas sp. PJ2 on bioremediation of PAHs and bacterial community composition in saline soil[J]. Chemosphere,2021,279:130427. doi: 10.1016/j.chemosphere.2021.130427
|
| [36] |
荚荣, 谢萍, 秦易. 固定化白腐真菌对多种染料脱色的研究[J]. 菌物系统,2003,22(2):308-313.
JIA R, XIE P, QIN Y. Study on decolorization of various dyes by immobilized white rot fungus schizophyllum sp[J]. Mycosystema,2003,22(2):308-313.
|
| [37] |
任静. 生物炭固定化菌剂强化修复多环芳烃污染土壤的机理研究[D]. 上海: 华东理工大学, 2020.
|
| [38] |
李莹. 白腐真菌菌剂及固定化酶对多环芳烃-石油烃复合污染土壤的修复研究[D]. 北京: 北京建筑大学, 2023.
|
| [39] |
周航. 固定化功能菌在多环芳烃污染土壤淋洗液中的降解特性研究[D]. 重庆: 重庆大学, 2020.
|
| [40] |
李明旭. 海带渣生物炭固载降解菌在PAHs污染土壤修复中的应用[D]. 大连: 大连理工大学, 2022.
|
| [41] |
赵琦慧. 能源植物种子载体固定化微生物对多环芳烃污染土壤的联合修复[D]. 上海: 上海应用技术大学, 2022.
|
| [42] |
TIAN W J, ZHAO Y G, SUN H M, et al. The effect of irrigation with oil-polluted water on microbial communities in estuarine reed rhizosphere soils[J]. Ecological Engineering,2014,70:275-281. doi: 10.1016/j.ecoleng.2014.06.003
|
| [43] |
占海生. 磁性壳聚糖固定化漆酶对水和土壤中多环芳烃的降解作用[D]. 南京: 南京农业大学, 2021.
|
| [44] |
NI N, WANG F, SONG Y, et al. Mechanisms of biochar reducing the bioaccumulation of PAHs in rice from soil: degradation stimulation vs immobilization[J]. Chemosphere,2018,196:288-296. doi: 10.1016/j.chemosphere.2017.12.192
|
| [45] |
徐铭毅, 李维, 王丽萍. 镉胁迫下固定化菌系菲降解能力及环境适应性研究[J]. 环境污染与防治,2023,45(4):443-449.
XU M Y, LI W, WANG L P. Study on the environmental adaptability and ability of phenanthrene degradation under Cd stress by immobilized microbial system[J]. Environmental Pollution & Control,2023,45(4):443-449.
|
| [46] |
CHEN B L, YUAN M X, QIAN L B. Enhanced bioremediation of PAH-contaminated soil by immobilized bacteria with plant residue and biochar as carriers[J]. Journal of Soils and Sediments,2012,12(9):1350-1359. doi: 10.1007/s11368-012-0554-5
|
| [47] |
黄真真. 聚乙烯醇固定化白腐真菌对复合废水的处理及其机理研究[D]. 长沙: 湖南大学, 2015.
|
| [48] |
高秀荣, 林姗娜, 孙安琪, 等. 一株多环芳烃降解菌的筛选及其降解特性[J]. 环境工程学报,2020,14(6):1668-1678. doi: 10.12030/j.cjee.201908168
GAO X R, LIN S N, SUN A Q, et al. Screening and degradation characteristics of a PAHs-degrading bacteria[J]. Chinese Journal of Environmental Engineering,2020,14(6):1668-1678. doi: 10.12030/j.cjee.201908168
|
| [49] |
李静华. 固定化微生物强化修复石油污染土壤的研究[D]. 广州: 华南理工大学, 2017.
|
| [50] |
王新, 李培军, 宋守志, 等. 固定化微生物对土壤中多环芳烃的降解[J]. 东北大学学报(自然科学版),2006,27(10):1154-1156. doi: 10.3321/j.issn:1005-3026.2006.10.024
WANG X, LI P J, SONG S Z, et al. Degradation of polycyclic aromatic hydrocarbons in soils by immobilized microorganisms[J]. Journal of Northeastern University (Natural Science),2006,27(10):1154-1156. doi: 10.3321/j.issn:1005-3026.2006.10.024
|
| [51] |
张松. 生物质炭固定化融合菌株的制备及对多环芳烃的去除研究[D]. 太原: 中北大学, 2018. □
|
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