Considering the poor biodegradability of the refractory pharmaceutical park tail water, activated sludge adsorption, O
3 oxidation and adsorption of activated sludge coupled with O
3 were separately used to pre-treat pharmaceutical tail water, and the test parameters were optimized. The results showed that separate activated sludge adsorption method had a certain ability to remove organic matter, but it could not effectively improve the biodegradability of the wastewater; both the COD
Cr removal efficiency and the biodegradability of wastewater were greatly improved by O
3 oxidation and adsorption of activated sludge coupled with O
3. It was verified that activated sludge coupled with O
3 process was an effective pretreatment technology, the COD
Cr removal efficiency could reach 37.90%, and the biodegradability was greatly improved, with BOD
5/COD
Cr increasing from 0.020 to 0.141, and its processing cost is more economical.