Abstract: The high-throughput sequencing was used to study the microbial community structure and distribution of sediment and reed rhizosphere in different types of constructed wetlands (tributary inlet wetland, pond wetland and oxbow lake wetland) in Panjin of Liaohe Conservation Area. The results showed that the microbial diversity, abundance and optical transform units (OTUs) of the three kinds of wetlands sediment were higher than that of reed rhizosphere. The diversity was affected by season, and the diversity of samples in autumn was higher than that in spring. The structure and distribution of microbial communities were roughly similar. Proteobacteria was the dominant species with the highest relative abundance. Gram-negative bacteria, mainly proteobacteria, played an important role in the purification of constructed wetlands. The class and relative abundance of Proteobacteria in different types of constructed wetlands were different. The Alphaproteobacteria, Deltaproteobacteria and Gammaproteobacteria were dominated in the tributary inlet wetland, while the Gammaproteobacteria, and Alphaproteobacteria were dominated in the pond wetland. In the oxbow lake wetland, the microorganism distribution of different classes in wetland was uniform.