Abstract: As an important part of the source prevention system, the negative list for industrial environmental access (hereinafter referred to as negative list) system has become a significant measure of current environmental management, playing an important role in guiding and restraining industrial development, coordinating economic development and environmental protection, and promoting green development. With a focus on the implications analysis and drafting principles, the technical route of the negative list for industrial environmental access was constructed, which included industrial environmental rationality analysis, key control industry screening method based on resources and environmental carrying capacity, and environmental management requirements for industrial access. The classification criteria of prohibited and restricted industries were expounded in details, and the environmental management requirements for industrial access presented from project scale, layout and location, resource development efficiency, pollution control, environmental risk prevention and control and so on. At last, the overall framework of the negative list was given.