Optimization of conditions for purification of wastewater treatment plant effluent by microalgae-bacteria symbiotic system
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摘要:
城镇污水处理厂尾水排入水体后,尾水中的氮、磷仍易引起受纳水体富营养化问题,开展尾水深度处理以进一步去除氮、磷营养物质具有现实意义。通过试验研究了不同菌藻共培养对氮、磷的去除效果,筛选出优势菌藻组合,采用响应面法研究了通气量、光波长、细菌接种量对氮、磷去除效果的交互影响,提出最优参数组合,并建立菌藻共生系统,进行验证试验。结果表明:不同菌藻组合中,蛋白核小球藻-地衣芽胞杆菌-恶臭假单胞菌共培养组对TN、TP的去除效果较好;此菌藻共生系统在蓝光、通气量为1.8 L/min及细菌接种量为20%(体积比)条件下,TN去除率最大可达93.7%,1 d 后TP基本上完全被去除;在蓝光、通气量为2.0 L/min及细菌接种量为5%条件下,2 d后氮去除率可达98.4%;在红光、通气量为2.0~3.0 L/min及细菌接种量为10%~20%条件下,2 d后氨氮可完全被去除。菌藻共生系统对氮、磷去除效果的最优参数组合为蓝光、通气量为1.8 L/min及细菌接种量为20%,最优参数组合验证的结果与预测值相符,系统出水符合GB 3838—2002《地表水环境质量标准》Ⅴ类水质标准,可为菌藻共生系统的实际应用提供理论基础。
Abstract:The effluent from municipal wastewater treatment plants contains nitrogen and phosphorus compounds, and the effluent discharged into the water body is still easy to cause eutrophication. The study on advanced treatment of effluent to further remove nitrogen and phosphorus pollutants is of practical significance. The removal effect of co-cultivation of different bacteria and microalgae on nitrogen and phosphorus was studied in the experiment, and the combination of dominant bacteria and microalgae was screened out. Response surface methodology (RSM) was used to study the interactive effects of light wavelength (LW), aeration rate (AR) and the inoculation ratio of bacteria (IR) on the removal effect of nitrogen and phosphorus. The optimal parameter combination was proposed, a microalga-bacterial symbiosis system was established, and a verification experiment was carried out. The results showed that among different combinations of algae and bacteria, the co-culture group of Chlorella proteinosa, Bacillus licheniformis and Pseudomonas putida had the best removal effect on TN and TP. When LW was blue light, AR was 1.8 L/min, and IR was 20%, TN maximum removal rate could reach 93.7%, and TP was basically completely removed after 1 day. When LW was blue light, AR was 2.0 L/min, and IR was 5%, the removal rate of ammonia nitrogen after 2 days was 98.4%. When LW was red light, AR was 2.0-3.0 L/min, and IR was 10%-20%, ammonia nitrogen after 2 days was basically completely removed. When LW was blue light, AR was 1.8 L/min, and IR was 20%, it was the optimal parameter condition for nitrogen and phosphorus removal efficiency. The verification results of the optimized parameter combination were consistent with the predicted values. The effluent of the system met Class Ⅴ of Environmental Quality Standards for Surface Water (GB 3838-2002), which provided a theoretical basis for the practical application of the microalgae-bacteria symbiotic system.
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表 1 响应面优化试验的因子及水平设计
Table 1. Code and levels for testing variables of response surface methodology experiments
试验组号 光波长(A) 通气量(B)/(L/min) 细菌接种量(C)/% 1 红光 2 5 2 蓝光 2 10 3 蓝光 2 10 4 蓝光 2 10 5 蓝光 3 5 6 蓝光 1 5 7 红光 3 10 8 蓝光 1 20 9 白光 3 10 10 白光 2 20 11 蓝光 3 20 12 白光 1 10 13 白光 2 5 14 蓝光 2 10 15 蓝光 2 10 16 红光 1 10 17 红光 2 20 注:光波长为白光时,A=−1;为蓝光时,A=0;为红光时,A=1。 表 2 响应面试验结果
Table 2. Response surface methodology experimental results
试验
组号去除率/% TN TP 氨氮 1 d 的TP 2 d的氨氮 1 89.95 97.39 98.17 92.52 96.25 2 93.41 99.55 98.39 85.92 98.32 3 92.09 98.48 97.57 90.51 96.36 4 93.98 98.64 98.69 81.83 94.97 5 88.20 97.81 98.49 59.84 89.12 6 84.46 99.04 98.46 60.08 86.40 7 89.19 97.26 98.31 68.49 96.36 8 91.15 97.24 98.15 87.64 67.02 9 89.60 98.07 97.90 44.55 56.94 10 92.41 99.71 98.42 94.37 43.43 11 89.86 98.83 98.30 82.82 90.52 12 82.87 98.33 98.62 38.28 46.92 13 86.73 97.33 98.50 35.29 74.05 14 91.28 98.13 98.50 85.21 93.49 15 91.19 97.39 97.65 86.02 95.43 16 89.51 98.68 98.21 56.58 86.62 17 92.20 98.06 98.15 92.17 94.33 表 3 响应值为TN、2 d的氨氮、1 d的TP去除率的方差分析结果
Table 3. Anova results of removal rate of TN, ammonia nitrogen after 2 days and TP after 1 days
方差来源 TN去除率 2 d的氨氮去除率 1 d的TP去除率 F P F P F P 模型 14.11 0.001 1 59.80 < 0.000 1 21.20 0.000 3 A 7.09 0.032 3 316.34 < 0.000 1 22.82 0.002 0 B 5.54 0.050 8 34.14 0.000 6 0.40 0.548 4 C 31.00 0.000 8 32.80 0.000 7 45.75 0.000 3 AB 11.63 0.011 3 0.002 0 0.965 4 0.24 0.636 6 AC 3.49 0.104 1 18.68 0.003 5 23.83 0.001 8 BC 7.18 0.031 6 11.63 0.011 3 0.52 0.494 7 A2 7.14 0.031 9 99.06 < 0.000 1 25.17 0.001 5 B2 41.65 0.000 3 34.11 0.000 6 51.40 0.000 2 C2 7.29 0.030 6 0.88 0.379 4 0.54 0.486 8 失拟项 0.24 0.863 2 5.76 0.061 9 6.62 0.049 7 -
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