Abstract: Ecological modern city is an important support for the construction of national ecological civilization, and the construction of ecological security pattern is an important basis for the realization of urban ecological priority and green development. Taking Wuhan City as an example, the importance of ecosystem services and ecological sensitivity were used to identify the ecological sources, the ecological corridor was extracted through the minimum cumulative resistance model, the ecological nodes were identified, and the ecological security pattern of Wuhan City was constructed. The results showed that there were 98 ecological sources in Wuhan City, covering an area of 2 214.7 km² and accounting for 25.82% of the total area of the city. The main type of ecological source was water, and it conformed to the ecological characteristics of water resources of rivers and lakes in Wuhan City with crisscross rivers and scattered lakes. 80 important ecological corridors were extracted, with a length of 928 km. The corridors were staggered to form a stable ecological network structure of "five horizontal and four vertical". 6 ecological nodes, or the key location of the ecological corridor to ensure the overall connectivity of ecological space, were identified. Finally, the network system of comprehensive ecological security pattern in Wuhan City was constructed. The construction of ecological patterns based on the model of "source - ecological corridor - ecological node" could identify important ecological functional areas, clarify regional ecological security risks, and provide a scientific basis for the protection of urban ecological security and regional sustainable development of Wuhan City.