改性蓝藻生物炭促进生物电化学系统阴极氢自养反硝化过程研究

熊江磊; 罗嘉豪; 严群

doi:10.12153/j.issn.1674-991X.20210362

改性蓝藻生物炭促进生物电化学系统阴极氢自养反硝化过程研究

doi: 10.12153/j.issn.1674-991X.20210362

1.
中国电子系统工程第二建设有限公司
2.
江南大学环境与土木工程学院

基金项目: 江苏省“双创博士”企业创新类人才项目（〔2019〕30279号）；江苏省“双创人才”项目（〔2020〕10342号）

详细信息

作者简介:
熊江磊（1984—），男，高级工程师，博士，研究方向为工业废水的处理及资源化，xiongjianglei@hotmail.com

中图分类号: X52,X703
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出版历程
- 收稿日期: 2021-07-29

Research on modified cyanobacterial biochar promoting cathodic hydrogen autotrophic denitrification in bioelectrochemical system

1.
China Electronics System Engineering NO.2 Construction Co., Ltd.
2.
School of Environmental and Civil Engineering, Jiangnan University

摘要

摘要:
以太湖蓝藻为原料制备不同种类的活性生物炭，并将其投入到生物电化学系统（BES）的阴极促进氢自养反硝化。通过扫描电镜、能谱仪和傅里叶红外光谱对未经改性（ABC-800）、硝酸改性（ABC-800N）和KOH改性（ABC-800K）3组蓝藻生物炭进行观察，并与不加入蓝藻生物炭的对照组进行比较，以考察生物炭促进BES生物阴极的反硝化过程中的电子传递机制。结果表明：ABC-800N表面的N、O元素含量最高，同时与电子传递能力及生物相容性相关的共轭醌、酮结构的丰度也最高；将蓝藻生物炭投加至BES的非生物阴极中可提高阴极的脱氮效率，ABC-800N投加量为0.5 g时，7 d内脱氮效率达到最高，为96.0%，而对照组仅为29.6%；高通量测序表明，ABC-800N组的优势菌属为Thauera、JGI_0001001_H03、Thiobacillus、Denitratisoma等。
- 生物电化学系统(BES) /
- 氢自养反硝化 /
- 蓝藻生物炭 /
- 高通量测序
Abstract:
Different kinds of active biochar were prepared from cyanobacteria in Taihu Lake and put into the cathode of bioelectrochemical system (BES) to promote hydrogen autotrophic denitrification. Three groups of cyanobacterial biochar of Unmodified (ABC-800), modified by nitric acid (ABC-800N) and modified by KOH (ABC-800K) were observed by scanning electron microscopy (SEM), energy dispersive spectrometer (EDS) and Fourier transform infrared spectroscopy (FTIR), and were compared with the control group without the addition of cyanobacteria biochar, to investigate the electron transfer mechanism in denitrification process of biochar promotes BES biocathode. The results showed that the contents of nitrogen and oxygen on ABC-800N surface were the highest, and the abundance of conjugated quinone and ketone structures related to electron transport capacity and biocompatibility was also the highest. Adding cyanobacteria biochar to the abiotic cathode of BES could improve the nitrogen removal efficiency of the cathode. After adding 0.5 g ABC-800N into the BES abiotic cathode, the nitrogen removal efficiency reached the highest within 7 days, which was 96.0%, while the control group was only 29.6%. High-throughput sequencing showed that the dominant bacteria in ABC-800N group were Thauera, JGI_0001001_H03, Thiobacillus, and Denitratisoma, etc.
- bioelectrochemical system(BES) /
- hydrogenotrophic denitrification /
- cyanobacterial biochar /
- high-throughput sequencing

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图 1 3种生物炭材料的表面形貌

Figure 1. Surface morphology of the three biochar materials

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图 2 3种生物炭材料表面N元素含量分布

Figure 2. Distribution of N element content on the surface of three biochar materials

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图 3 3种生物炭材料表面O元素含量分布

Figure 3. Distribution of O element content on the surface of three biochar materials

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图 4 3种生物炭的N₂吸附-脱附曲线和孔径分布

Figure 4. Nitrogen adsorption-desorption isotherms and pore width distribution of the three materials

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图 5 不同生物炭材料的FTIR谱图

Figure 5. FTIR spectra of different biochar materials

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图 6 2 组非生物阴极硝氮、亚硝氮浓度随时间的变化

Figure 6. Variation of nitrate and nitrite concentrations with time in two groups of abiotic cathodes

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图 7 投加3种生物炭材料后生物阴极硝氮、亚硝氮浓度随时间的变化

注：柱状代表亚硝氮浓度；点状代表硝氮浓度。

Figure 7. Variation of nitrate and nitrite concentrations with time in biological cathode under the addition of three biochar materials

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图 8 添加不同生物炭材料时反应器内微生物种群相对丰度

Figure 8. Relative abundance of microbial population in reactors with different biochar materials addition

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表 1 3种生物炭材料表面元素含量

Table 1. Surface element contents of the three biochar materials %　

生物炭	C	N	O	K
ABC-800	86.88	6.88	6.24
ABC-800N	67.61	13.27	19.12
ABC-800K	76.78	7.04	15.98	0.2

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表 2 投加不同生物炭材料后各组反应器微生物的共有OTU在门水平的相对丰度

Table 2. Relative abundance of shared OTU of microorganisms in each reactor at phylum level after different biochar materials addition %　

细菌门	对照组	ABC-800	ABC-800N	ABC-800K
Proteobacteria	52.15	37.63	30.96	36.02
Firmicutes	2.63	4.37	4.26	5.38
Chloroflexi	8.32	9.00	11.51	8.62
Bacteroidota	12.91	27.49	28.88	21.89
Actinobacteriota	3.52	9.78	9.33	7.68
Synergisteota	4.20	0.30	0.99	0.56
Acidobacteriota	0.55	2.89	9.08	4.94

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