Abstract: Human activities have an important impact on the ecosystem and its service value. Taking Changzhou City, Jiangsu Province as an example, using six periods of land use data from 1995 to 2018 and based on 2 km×2 km grid, the ecosystem service value and human activity intensity were calculated, and the temporal and spatial variation characteristics and their correlation were analyzed. The results showed that from 1995 to 2018, the cultivated land area in Changzhou City decreased by 19.5%, while the water area and construction land area increased by 11.6% and 93.7%, respectively. The value of ecosystem service experienced a process of first increasing, then decreasing and overall increasing, with an increase of 2.955 billion yuan. The increase of value mainly came from the transformation of cultivated land into water, while the decrease of value mainly from the transformation of cultivated land into construction land. The intensity of human activities had increased significantly, especially in the urban area of Changzhou City. There was a significant negative correlation between human activity intensity and ecosystem service value, and this negative correlation was increasing.