Abstract: With the in-depth advancement of the rural revitalization strategy and the increasingly stringent sewage discharge standards, higher requirements have been placed on rural domestic sewage treatment technology. The mobile biofilm technology was optimized and improved when an upward-shunting multi-media biofilm operation system was developed. The treatment effect of the technology for actual rural domestic sewage was investigated, and its promotion and application value was discussed. The results indicated that the carbon source in the sewage system was utilized efficiently for denitrification. With the experimental conditions of hydraulic retention time longer than 0.15 d, the gas-to-water ratio greater than 3:1 and 1.50 times of nitrification liquid reflux ratio simultaneously, the removal rate of SS, TN, NH₄ ⁺-N, COD_Cr and TP in sewage reached 96.23%, 47.33%, 78.89%, 79.38% and 80.77%, respectively, without sludge return. Besides, the effluent quality was better than Level 1 A standard of Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant (GB 18918-2002).