Abstract: To explore whether agricultural wastes can adsorb antibiotics and whether there is an interaction between different antibiotics, the physicochemical characteristics analysis of corncob, Fourier Infrared Spectroscopy analysis, adsorption kinetics, and adsorption isotherms analysis were used to study the adsorption effect and mechanism of sulfamethoxazole (SMX) and trimethoprim (TMP) on corncob. The results showed that SMX and TMP adsorption capacities on corncob were 131.18 and 358.75 mg/kg in the system with single antibiotic （the initial concentration was 10 mg/L） at 25 ℃ and corncob concentration of 25 g/L , and they were 131.02 and 358.74 mg/kg in the coexistence system with both SMX and TMP（the initial concentration both were 10 mg/L）, respectively. There was non-interactive adsorption between SMX and TMP in the coexistence system. The adsorption kinetics of SMX and TMP were better represented by the pseudo-second-order kinetic model in the two systems. Both Langmuir and Freundlich isotherms were well fitted with the SMX adsorption process, and the adsorption of TMP was described well by the Freundlich isotherm. SMX was mainly adsorbed by electrostatic interaction and π-π stacking mechanism, and the electrostatic interaction played the main role. The adsorption of TMP was mainly due to the hydrophobic distribution, π-π stacking, and hydrogen bonding.