Abstract: List of ecological environment permits originates from the integration of Three Lines delineation management and control results, which is a significant export for each environmental control unit to implement the bottom-line thinking and the hard constraints on ecological environment protection. It is worth pondering and discussing how the ecological environment permits list can be used, applicable and effective in the actual environmental management. With the characteristics of industrial park management control units, the ecological environment permits list system of Hunan Province is among the first to be issued and fully implemented in China. Taking the achievements of Hunan Province as an example, the compilation principles, technical ideas, characteristics of practice and application effect of the environmental permits list were systematically expounded. By summing up the problems, some thoughts and suggestions on optimizing the ecological environment permits list were put forward, including 1) to clarify the legal status and hierarchical positioning of Three Lines One Permit (TLOP), find out the functional positioning, and form a situation of multi-department coordinative promotion; 2) to strengthen the target support function of the ecological environment permits list and ensure its scientific application; 3) to standardize the form of ecological environmental permit list and coordinate multi-factor management control; and 4) to introduce supporting mechanism and guide the dynamic update of TLOP.