Abstract: Revealing the spatiotemporal evolution characteristics of habitat quality and their influencing factors can provide reference basis for regional sustainable development. Based on the land use data in 2000, 2010 and 2020, InVEST model, landuse transfer matrix and optimal parameters-based geographical detector (OPGD) were used to comprehensively analyze the spatiotemporal evolution and driving factors of habitat quality in Fuzhou City. The results revealed that the habitat quality index of Fuzhou City in 2000, 2010 and 2020 was 0.812, 0.806 and 0.793, respectively. The area of habitat improvement was less than the area of habitat deterioration. Habitat conditions in the main urban area of Fuzhou and the southeast coastal region were in urgent need of improvement. 3 km was the best optional spatial unit for this study, the optimal data discretization classification number is 6, and the natural break method better explained the explanatory power of the driving factors. Elevation, slope and night-time lights were the main influencing factors of spatial variation in habitat quality in Fuzhou City, and the interaction of slope and nighttime lights had the strongest explanation for habitat quality changes.