Abstract: Based on the livestock and poultry breeding data of Yunnan Province in 2023, we estimated the livestock and poultry liquid manure production in Yunnan Province. Combined with the anaerobic fermentation mode of liquid manure and based on irrigation volume, we estimated the absorption capacity of the land polluted by livestock and poultry liquid manure in Yunnan Province. The results showed that livestock and poultry liquid manure production in Yunnan Province was 20 584.50 t/a, which could substitute 754.68 t/a of urea and 20.09 t/a of calcium superphosphate after proper storage and fermentation. When calculating the supporting area of farmland based on irrigation volume, more than 90% of cities (prefectures) in Yunnan Province would not be able to dispose of the manure generated locally, and 16%-74% of the manure discharged needed to be processed to meet the discharge standard. It was necessary to increase the degree of harmless treatment and reduce the dilution factor of liquid manure for returning to the field, in order to achieve a full return and reduce the up-to-standard discharge of liquid manure in Yunnan Province. Based on the current situation of Yunnan Province, we proposed a technical model of microbial synergistic liquid manure acidification, storage and fermentation to increase the harmlessness degree and nutrient preservation capacity of liquid manure, which could provide a new technical approach for the resource utilization of liquid manure treatment in Yunnan Province.