Abstract: This study investigates 33 long-term fertilization positioning points in Beijing. The impact of fertilization measures, fertilization years, type of fertilization and type of planting on the content of soil organic matter, total N, alkali-hydrolyzable N, available P, and available K were analyzed in this study. The results revealed that long-term fertilization significantly increased the nutrient content in farmland. The average soil organic matter increased from 13.8 g/kg in 2008 to 25.05 g/kg in 2022, with an annual increase of 0.75 g/kg. At the same time, the growth rates of available P and available K were very high, with the mean growth rates of 158.38% and 158.67%, respectively. Compared to no fertilization, the application of organic fertilizer, and the combined application of organic and inorganic fertilizers significantly increased soil organic matter and total N content. However, only the combined application of organic and inorganic fertilizers significantly increased the content of alkali-hydrolyzable N, available P, and available K. For a continuous input of organic fertilizer nutrients up to 60 kg/mu, the organic matter in farmland increased with the increase in nutrient input. Similarly, When the total fertilizer nutrient (N+K₂O+P₂O₅) input was within 70 kg/mu, the soil nutrient content increased with the increase in nutrient input. Furthermore, the soil nutrient content of farmland in the plain agricultural area was the highest, followed by the suburban agricultural area and the mountain ecological conservation area. Moreover, the nutrient content in vegetable fields was significantly higher than that in grain fields and orchards (p < 0.05), with no significant difference between the latter two. This study provides fundamental data for agricultural fertilization practices and offers scientific guidance and reference for the rational fertilization of arable land in Beijing.