Abstract: Soil heavy metal pollution is one of the key environmental issues of concern in the Yellow River Basin. Reducing the bioavailability of heavy metals in soil and enhancing the resistance of plants to heavy metals are common strategies for addressing low to moderate levels of contamination. Biochar can fix heavy metals and lower the bioavailability of heavy metals, and arbuscular mycorrhizal fungi (AMF) can enhance plant resistance to heavy metals through symbiosis with plant roots. This study investigated the effects of combined biochar and AMF application on the growth, lead (Pb) absorption, and rhizosphere bacterial community of Paspalum natatu through pot experiments. Four treatments were set up under Pb pollution levels of 0, 200, and 2 000 mg/kg: control (CK), biochar addition (JBC), AMF addition (JAMF), and simultaneous addition of both (BM). The results showed that both BC and AMF promoted the growth of Bahiagrass. BC decreased the translocation coefficient of Pb in plants, while AMF increased it. Under the Pb application rate of 200 mg/kg, the BM treatment significantly promoted plant growth, with plant height, root length and biomass being 17.20%、31.70%、608.61% higher than those in CK (P<0.05), respectively. Under the Pb application rate of 2 000 mg/kg, the BM treatment did not significantly improve plant growth. This was probably because AMF enhanced Pb absorption, leading to a significant increase in Pb content within plants (stem and leaf Pb content increased by 39.85%, and root Pb content increased by 7.64%), and offset the growth promotion effects of BC and AMF. Bacterial community analysis revealed that the combined application of AMF and biochar significantly altered the bacterial community structure under high 2 000 mg/kg Pb, enhancing the α-diversity (Chao1 value and Simpson index) and stability of the soil bacterial community. This study provides a theoretical basis and technical support for the combined application of biochar and AMF in the remediation of heavy metal-polluted soils.