Abstract: Given the inconvenience of collecting and transporting kitchen waste from remote towns and scattered locations, the key to enhancing the efficiency of in-situ treatment lies in constructing an efficient degrading microbial agent. We screened four bacteria, two actinomycetes, and two fungi from environmental samples using extracellular enzyme activity as the screening indicator, and developed a composite microbial agent capable of synergistically degrading starch, protein, lipids, and cellulose. To enhance the efficacy of the microbial agent, we employed response surface methodology to synergistically optimize the mixed fermentation and solid-carrier adsorption processes, thus successfully preparing a rice husk-based solid microbial agent. Bench-scale verification was conducted using a small-scale bioreactor unit. The results demonstrated that under the optimal mass ratio of food waste to microbial agent of 20∶3, the wet and dry matter reduction rates reached 88.21% and 80.89%, respectively, after only 24 hours of treatment. Compared to commercially available EM microbial agents, this composite microbial agent not only improved the dry matter degradation rate by approximately 13%, but also exhibited good stability during continuous operation for 15 days. This research verifies the application potential of the specific microbial community in in-situ treatment scenarios, and provides strong technical support for the rapid reduction and resource recovery of kitchen waste at the source.