Abstract: With the environmental pollution problems becoming increasingly prominent in China, a quantity of innovated technologies are urgently needed for the improvement of the control effect of environmental pollution. However, the present expert-based evaluating system of pollution prevention and control technologies is difficult to scientifically and objectively show the actual capability of technologies, and to promote the dissemination and application of new technologies. In order to establish the scientific system of environmental technology verification (ETV), a sampling study on the verification of the biological technologies of water pollution prevention and control was performed. The verification index system and methods of biological technologies of water pollution prevention and control were established, and a demonstration test concerning the treatment of nylon wastewater by a biological process conducted. The results showed that setting a three-month period of verification was reasonable for demonstrating the operating effect of the biological treatment technology under various operation conditions. The indexes on water quality, secondary pollution, process operation and techno-economy were tested. It was found that the statistical data obtained in the process of test could objectively and effectively reveal the actual capability of pollution prevention and control technologies. Some suggestions on further improving index system and verification test methods of the biological technologies of water pollution prevention and control were proposed finally.