Abstract: The priority organic pollutants in groundwater were screened based on foreign and domestic research experience to meet the demand of monitoring and assessment in groundwater pollution of China. Acute toxicity, reproductive toxicity, carcinogenicity, endocrine disrupting activity, persistence, bioaccumulation, migration and occurrence frequency of pollutants were selected as assessment indicators. Analytic Hierarchy Process combined with Weighted Mark Method was used in quantitative screening of priority organic pollutants in groundwater. Sixteen categories including 85 organic pollutants were tentatively selected on the priority pollutants list, which contains 16 kinds of halogenated hydrocarbons, 13 kinds of polycyclic aromatic hydrocarbons, 20 kinds of pesticides and herbicide, 7 kinds of phenols, six kinds of chlorinated benzene, 6 kinds of BTEX, three kinds of nitrobenzene, 3 kinds of esters, 2 kinds of aldehydes, 2 kinds of anilines, 2 kinds of nitrosamines and dichloroacetic acid, acrylonitrile, PCBs, MTBE and acrylamide. The screening focused on considering the organic substances that widely distributed in the environment with high concentration and harmfulness to the environment.