辽河保护区七星湿地水体溶解性有机物紫外吸收光谱研究

Study on dissolved organic matter of surface water using UV-visible spectroscopy in Qixing wetland, Liaohe Reserve

  • 摘要: 双组分模型假定吸收紫外光的溶解性有机物(dissolved organic matter,DOM)由2种组分(A和B)组成,组分A对紫外光有强烈的吸收,主要为芳香族化合物且具有疏水性;组分B对紫外光吸收较弱,具有亲水性。对七星湿地水样DOM进行紫外光谱扫描,采用双组分模型对溶解性有机碳(dissolved organic carbon,DOC)浓度进行模拟预测,并研究组分A所占比例(fA)与DOM性质的关系。结果表明,双组分模型可以较好地预测七星湿地水体中DOC的浓度(rmsd为2.18 mg/L,R2为0.963),相应模型参数EA,254为95.04 L/(g·cm),EB,254为0.15 L/(g·cm),EA,340为30 L/(g·cm),EB,340为0。fA随着水样中DOC浓度升高呈下降趋势,并且与特征吸光度(special UV absorbance, SUVA)SUVA254、SUVA280有较好的线性关系(R2>0.900)。fA可以反映DOM中不饱和共轭双键、苯环类化合物及芳香族化合物的变化,但不能反映DOM的分子量大小。因此,利用双组分模型可以实现对DOM定性及定量的分析。

     

    Abstract: The Two-component model assumes that UV-absorbing dissolved organic matter (DOM) consists of two components (A and B). Component A absorbs UV light strongly, therefore being presumed to possess aromatic chromophores and hydrophobic character, while component B absorbs weakly and can be assumed hydrophilic. The water samples in Qixing wetland were scanned by UV-visible spectra, the dissolved organic carbon (DOC) concentrations estimated by two-component model, and then the relationship between fA (the fraction of component A) and DOM properties studied. The results showed that: the two-component model provided accurate estimates of DOC concentrations in water of Qixing wetland (rmsd=2.18mg/L, R2=0.963), the corresponding model parameter values were EA,254=95.04 L/(g·cm), EB,254=0.15 L/(g·cm), EA,340=30 L/(g·cm), EB,340=0, respectively. fA values show a declining trend with the increase of the DOC concentration. There are good linear relationships (R2>0.900) between fA and special UV absorbance SUVA254, SUVA280. fA can reflect the unsaturated conjugated double bonds, benzene ring compounds and aromatic compounds in DOM, but it cannot reflect the molecular weight size of the DOM. Accordingly, two-component model can be used for qualitative and quantitative analysis of DOM.

     

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