Abstract: Based on the constraint mechanism of water ecological function to industrial distribution, the water ecological constraints were decomposed into water resources constraint and water environmental constraint. A water ecological constraint zoning index system was constructed, covering not only baseline factors of water resources and water environment, but also stress factors of water resources and water environment produced by industrial distribution. A water ecological constraint zoning method was also presented. Taken Tieling city as an example, water ecological zoning was carried out by applying the index system. The city was divided into five types of water ecological constraint zones, including high water resources constraint and high water environmental constraint, medium water resources constraint and medium water environmental constraint, low water resources constraint and high water environmental constraint, low water resources constraint and medium water environmental constraint, and low water resources constraint and low water environmental constraint. Finally, industrial distribution optimization countermeasures were proposed on different types of restricted, upgraded and encouraged industries and industrial development focuses at different water ecological constraint zones.