Abstract: Five substrates, including gravel, sandy soil, red soil, purple soil and paddy soil, were used to study the effects of the substrates on Ottelia acuminate seed germination, seedling growth, yield and water quality of with the pilot scale test. The results showed that the rate of seed germination was 74.25% in the red soil and 73.68% in the paddy soil, respectively, which was high than that in sandy soil and purple soil. The growth conditions results showed that paddy soil and purple soil were more favorable to the growth of Ottelia acuminate seedlings than the three substrates. The cumulative results showed that the total number of flowering flowers in purple soil was 22, the number of stem was 32/m ², and the weight of stem was 145.10 g. The total weight of the stems was followed by paddy soil (117.60 g). The removal results showed that under the five substrates the Ottelia acuminate economic wetland had good effect on NH_4^+-N、NO_3^--N and TN removal, while red soil had good effect on TP removal and paddy soil had poor effect.