Abstract: Through the up-flow anaerobic sludge bed(UASB)simulation system, the effects of different environmental factors on the refractory organics and nitrogen synchronization removal in landfill leachate were studied. Then the removal efficiency of organic compounds and nitrogen in leachate was analyzed by using the humus in the UASB simulation system. Results show that under the pH of 7.9 to 8.4, ORP of -200 mV to -100 mV and -300 mV to -200 mV, the COD removal rate was 92.05% and 95.08%, and total nitrogen removal rate was 62.03% and 66.73%, respectively. The three-dimensional fluorescence spectrum analysis showed that humus in the leachate class material showed a trend of decrease, indicating that the denitrification bacteria may use the refractory organic matter in garbage leachate for the denitrification. The optimum ORP range was -300 mV to -100 mV for COD and total nitrogen removal, which realized the simultaneous removal of organic matter and nitrogen in landfill leachate.