污泥膨胀对反硝化中试反应器效能及菌群结构的影响

许子聪; 李海松; 胡培基

doi:10.12153/j.issn.1674-991X.2018.10.300

污泥膨胀对反硝化中试反应器效能及菌群结构的影响

doi: 10.12153/j.issn.1674-991X.2018.10.300

^1. 郑州大学化工与能源学院,河南郑州 450001
^2. 知和环保科技有限公司,河南郑州 450001

详细信息

作者简介:
许子聪(1994—),女,硕士研究生,研究方向为污水生物脱氮, 929677679@qq.com

中图分类号: X703
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- 被引次数: 0
出版历程
- 收稿日期: 2018-08-20
- 刊出日期: 2019-03-20

The effects of filamentous sludge bulking on performance and bacterial community structure of pilot-scale denitrification reactor

^1. School of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450001, China
^2. Zhihe Environmental Science and Technology Co., Ltd., Zhengzhou 450001, China

摘要

摘要: 考察了丝状菌污泥膨胀对有效体积为10 m ³,流量为300 m ³/d中试规模反硝化反应器性能的影响,并利用高通量测序法分析了菌群结构的变化。在反应器正常运行66 d时,观测到污泥膨胀现象,污泥沉降性能明显恶化,SV30由30%增至85%,反应器内生物量也随之降低,污泥浓度由3 300 mg/L降至1 400 mg/L。镜检发现,污泥膨胀后出现大量的丝状菌,紧密的菌胶团受到破坏,说明此次污泥膨胀主要由丝状菌引起;高通量测序结果也证实了发硫菌属(Thiothrix)、纤毛属(Leptothrix)和束缚杆菌属(Haliscomenobacter)等丝状细菌在污泥膨胀后富集,由丝状菌类型和环境因素的变化推断高硫化物浓度和低溶解氧浓度是污泥膨胀的诱因。尽管发生了污泥膨胀和污泥流失,反应器反硝化性能并未降低,这可能与Methyloversatilis、氢噬胞菌属(Hydrogenophaga)和Thiothrix等具有反硝化功能的菌属丰度增大(分别为29.74%、1.69%和3.45%)有关。此外,菌群结构的变化也是导致功能基因丰度改变的原因之一。
- 污泥膨胀 /
- 反硝化 /
- 丝状菌 /
- 细菌 /
- 功能基因
Abstract: The effect of filamentous sludge bulking on the performance of a pilot-scale denitrification reactor with effective volume of 10 m ³ and flow rate of 300 m ³/d was investigated. The changes of bacterial community structure were analyzed by high throughput sequencing.When the reactor operated normally for 66 days, sludge bulking phenomenon was observed and sludge sedimentation performance deteriorated significantly; the SV30 rose from 30% to 85%, and the biomass in the reactor decreased, with the mixed liquid suspended solids (MLSS) decreasing from 3 300 mg/L to 1 400 mg/L. The microscopic examination found that a large number of filamentous micro-organisms extended out of the flocs, and the close bacterial micelles were destroyed, which indicated that the sludge bulking was mainly caused by filamentous bacteria. Furthermore, high-throughput sequencing confirmed the filamentous bacteria such as Thiothrix, Leptothrix and Haliscomenobacter were enriched after sludge bulking, and were changed by filamentous bacteria types and environmental factors, indicating that higher sulfide and lower DO may provided better conditions for sludge bulking. It was noted that the denitrification performance of the reactor was not affected by sludge bulking and sludge loss, which may attribute to the increase in abundance of denitrifiers such as Methyloversatilis, Hydrogenophaga and Thiothrix (29.74%, 1.69% and 3.45%, respectively). Besides, the shift of bacterial community structure might cause changes in the abundance of functional genes.
- sludge bulking /
- denitrification /
- filamentous /
- bacteria /
- functional genes

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参考文献(21)

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