三维荧光光谱表征Fe(Ⅱ)浓度对厌氧氨氧化过程的影响与平行因子分析

王嵘; 姚亮; 申慧彦; 余丽; 李卫华

doi:10.12153/j.issn.1674-991X.2019.09.120

三维荧光光谱表征Fe(Ⅱ)浓度对厌氧氨氧化过程的影响与平行因子分析

doi: 10.12153/j.issn.1674-991X.2019.09.120

王嵘^1,2,
姚亮^1,2,
申慧彦^1,2,
余丽^1,2,
李卫华^1,2, ,

^1. 安徽建筑大学环境与能源工程学院,安徽合肥 230022
^2. 水污染控制与废水资源化安徽省重点实验室,安徽合肥 230022

详细信息

作者简介:
王嵘(1989—),女,硕士,主要研究方向为废水生物处理, ronger@ahjzu.edu.cn

通讯作者:
李卫华 E-mail: liweihua9@126.com

中图分类号: X703
计量
- 文章访问数: 242
- HTML全文浏览量: 74
- PDF下载量: 122
- 被引次数: 0
出版历程
- 收稿日期: 2019-07-08
- 刊出日期: 2019-11-20

Characterizing the effect of Fe(Ⅱ) dosage on anammox treatment process using excitation-emission matrix fluorescence spectroscopy and parallel factor analysis

WANG Rong^1,2,
YAO Liang^1,2,
SHEN Huiyan^1,2,
YU Li^1,2,
LI Weihua^{1,2
, ,}

^1. School of Environmental and Energy Engineering, Anhui Jianzhu University, Hefei 230022, China
^2. Key Laboratory of Water Pollution Control and Wastewater Reuse of Anhui Province, Hefei 230022, China

More Information

Corresponding author: Weihua LI E-mail: liweihua9@126.com

摘要

摘要: 考察Fe(Ⅱ)浓度对厌氧氨氧化过程的影响,并采用三维荧光光谱结合平行因子分析方法,解析厌氧氨氧化反应器出水中的荧光组分,探究外加Fe(Ⅱ)与反应器出水水质的关系。结果表明:随着Fe(Ⅱ)浓度从1.84 mg/L升至5.00 mg/L,N $H 4 +$ -N和N $O 2 -$ -N的去除率逐渐增加,表明增加进水Fe(Ⅱ)浓度可以提高微生物对底物的利用率;随着Fe(Ⅱ)浓度的增加,厌氧氨氧化菌的数量亦显著增加;厌氧氨氧化反应器出水的主要荧光组分是类蛋白质和类富里酸物质,随着Fe(Ⅱ)浓度的增加,反应器出水的类蛋白质荧光强度显著增强,表明在一定条件下,投加Fe(Ⅱ)可以促进厌氧氨氧化菌的生长。因此,利用三维荧光光谱法可以反映投加Fe(Ⅱ)对厌氧氨氧化性能的影响,进而反映反应器实际运行状况。
- 厌氧氨氧化 /
- 荧光光谱 /
- 平行因子分析 /
- Fe(Ⅱ); /
- 类蛋白质
Abstract: The effect of ferrous ions dosage on the anaerobic ammonia was investigated. The excitation-emission matrix (EEM) fluorescence spectroscopy combined with parallel factor analysis (PARAFAC) method was used to decompose the EEM fluorescence components in the effluent samples. The relationship between the added Fe(Ⅱ) and the effluent quality of the reactor was explored. The results showed that with the increase of ferrous ions concentration from 1.84 mg/L to 5.00 mg/L, the removal rates of N $H 4 +$ -N and N $O 2 -$ -N increased gradually, indicating that increasing the influent ferrous ions concentration could increase the utilization rate of substrate by microorganisms. The proportion of anammox bacteria increased significantly with the increase of ferrous ions dosage; protein-like substances and fulvic acid-like substances were the main fluorophores in the effluent of anammox reactor. With the increase of ferrous ions dosage, the protein-like fluorescence of the effluents increased significantly, which implicated that the dosage of ferrous ions would promote the growth of anammox bacteria under certain conditions. Therefore, EEM fluorescence spectroscopy could reflect the effect of ferrous ions on the performance of anaerobic ammonia oxidation, which then implicate the actual operation of the reactor.
- anammox /
- fluorescence spectra /
- parallel factor analysis(PARAFAC) /
- Fe(Ⅱ); /
- protein-like substance

HTML全文

参考文献(38)

[1]	MULDER A, van de GRAAF A A, ROBERTSON L A , et al. Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor[J]. FEMS Microbiology Ecology, 1995,16(3):177-184.
[2]	van de GRAAF A A, MULDER A, de BRUIJN P , et al. Anaerobic oxidation of ammonium is a biologically mediated process[J]. Applied Microbiology and Biotechnology, 1995,61(4):1246-1251.
[3]	STROUS M, HEIJNEN J J, KUENEN J G , et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J]. Applied Microbiology and Biotechnology, 1998,50(5):589-596.
[4]	BI Z, QIAO S, ZHOU J T , et al. Fast start-up of anammox process with appropriate ferrous iron concentration[J]. Bioresource Technology, 2014,170:506-512.
[5]	JETTEN M S M, WAGNER M, FUERST J , et al. Microbiology and application of the anaerobic ammoniumoxidation(‘anammox’)process[J]. Current Opinion in Biotechnololy, 2001,12(3):283-288.
[6]	van de STAR W R L, ABMA W R, BLOMMERS D , et al. Startup of reactors for anoxic ammonium oxidation:experiences from the first full-scale anammox reactor in Rotterdam[J]. Water Research, 2007,41(18):4149-4163.
[7]	HU B L, ZHENG P, TANG C J , et al. Identification and quantification of anammox bacteria in eight nitrogen removal reactors[J]. Water Research, 2010,44(17):5014-5020.
[8]	TANG C J, ZHENG P, WANG C H , et al. Suppression of anaerobic ammonium oxidizers under high organic content in high-rate anammox UASB reactor[J]. Bioresource Technology, 2010,101(6):1762-1768.
[9]	GILBERT E M, AGRAWAL S, KARST S M , et al. Low temperature partial nitritation/anammox in a moving bed biofilm reactor treating low strength wastewater[J]. Environmental Science & Technology, 2014,48(15):8784-8792.
[10]	LOTTI T, KLEEREBEZEM R, van LOOSDRECHT M C M. Effect of temperature change on anammox activity[J]. Biotechnology and Bioengineering, 2015,112(1):98-103.
[11]	LIU S T, YANG F L, XUE Y , et al. Evaluation of oxygen adaptation and identification of functional bacteria composition for anammox consortium in non-woven biological rotating contactor[J]. Bioresource Technology, 2008,99(17):8273-8279.
[12]	LOTTI T, KLEEREBEZEM R, van TAALMAN K C.et al. Anammox growth on pretreated municipal wastewater[J]. Environmental Science & Technology, 2014,48(14):7874-7880.
[13]	van de GRAAF A A, de BRUIJN P, ROBERTSON L A , et al. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor[J]. Microbiology, 1996,142:2187-2196.
[14]	QIAO S, BI Z, ZHOU J , et al. Long term effects of divalent ferrous ion on the activity of anammox biomass[J]. Bioresource Technology, 2013,142:490-497.
[15]	STROUS M, PELLETIER E, MANGENOT S , et al. Deciphering the evolution and metabolismof an anammox bacterium from a community genome[J]. Nature, 2006,440:790-794.
[16]	ZHAO R, ZHANG H M, LI Y F , et al. Research of iron reduction and the iron reductase localization of anammox bacteria[J]. Current Microbiology, 2014,69(6):880-887.
[17]	ZHANG J X, ZHANG Y B, LI Y. , et al. Enhancement of nitrogen removal in a novel anammox reactor packed with Fe electrode[J]. Bioresource Technology, 2012,114:102-108.
[18]	KARTAL B, van NIFTRIK L, KELTJENS J T , et al. Anammox-growth physiology,cell biology,and metabolism[J]. Advances in Microbial Physiology, 2012,60:211-262.
[19]	van NIFTRIK L, GEERTS W J C, van DONSELAAR E G , et al. Combined structural and chemical analysis of the anammoxosome:a membrane-bounded intracytoplasmic compartment in anammox bacteria[J]. Journal of Structural Biology, 2008,161:401-410.
[20]	COBLE P G . Characterization of marine and terrestrial DOM in seawater using excitation-emission matrix spectroscopy[J]. Marine Chemistry, 1996,51:325-346.
[21]	SHENG G P, YU H Q . Characterization of extracellular polymeric substances of aerobic and anaerobic sludge using three-dimensional excitation and emission matrix fluorescence spectroscopy[J]. Water Research, 2006,40:1233-1239.
[22]	LI W H, SHENG G P, LIU X W , et al. Characterizing the extracellular and intracellular fluorescent products of activated sludge in a sequencing batchreactor[J]. Water Research, 2008,42:3173-3181.
[23]	刘怡心, 李卫华, 申慧彦 , 等. 厌氧氨氧化反应过程的三维荧光光谱解析[J]. 环境工程学报, 2015,9(10):4680-4686. LIU Y X, LI W H, SHEN H Y , et al. Analysis of EEM fluorescence spectra of effluents from anammox reactor[J]. Chinese Journal of Environmental Engineering, 2015,9(10):4680-4686.
[24]	STEDMON C A, BRO R . Characterizing dissolved organic matter fluorescence with parallel factor analysis:a tutorial[J]. Limnology and Oceanography:Method, 2008,6:572-579.
[25]	ISHII S K L, BOYER T H . Behavior of reoccurring PARAFAC components in fluorescent dissolved organic matter in natural and engineered systems:a critical review[J]. Environmental Science & Technology, 2012,46:2006-2017.
[26]	NI B J, FANG F, RITTMANN B E , et al. Modeling microbial products in activated sludge under feast-famine conditions[J]. Environmental Science & Technology, 2009,43:2489-2497.
[27]	RUSCALLEDA M, SEREDYNSKA-SOBECKA B, NI B J , et al. Spectrometric characterization of the effluent dissolved organic matter from an anammox reactor shows correlation between the EEM signature and anammox growth[J]. Chemosphere, 2014,117:271-277.
[28]	魏复盛 . 水和废水监测分析方法[M]. 4版.北京: 中国环境科学出版社, 2002.
[29]	SAWAYAMA S . Possibility of anoxic ferric ammonium oxidation[J]. Journal of Bioscience and Bioengineering, 2006,101(1):70-72.
[30]	WEBER K A, URRUTIA M M, CHURCHILL P F , et al. Anaerobic redox cycling of iron by freshwater sediment microorganisms[J]. Environmental Microbiology, 2006,8(1):100-113.
[31]	KARTAL B, KUYPERS M M M, LAVIK G,et al. Anammox bacteria disguised as denitrifiers:nitrate reduction to dinitrogen gas via nitrite and ammonium[J]. Environmental Microbiology, 2007,9(3):635-642.
[32]	JARUSUTTHIRAK C, AMY G . Understanding soluble microbial products(SMP) as a component of effluent organic matter(EfOM)[J]. Water Research, 2007,41:2787-2793.
[33]	LASPIDOU C S, RITTMANN B E . A unified theory for extracellular polymeric substances,soluble microbial products,and active and inert biomass[J]. Water Research, 2002,36:2711-2720.
[34]	ZHOU Z B, MENG F G, LIANG S , et al. Role of microorganism growth phase in the accumulation and characteristics of biomacromolecules(BMM)in a membrane bioreactor[J]. RSC Advances, 2012,2:453-460.
[35]	CHEN W, WESTERHOFF P, LEENHEER J A , et al. Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter[J]. Environmental Science & Technology, 2003,37:5701-5710.
[36]	WANG Z P, ZHANG T . Characterization of soluble microbial products(SMP)under stressful conditions[J]. Water Research, 2010,44:5499-5509.
[37]	ARUNACHALAM R, SHAH H K, JU L K . Monitoring aerobic sludge digestion by online scanning fluorometry[J]. Water Research, 2005,39:1205-1214.
[38]	NI B J, ZENG R J, FANG F , et al. A novel approach to evaluate the production kinetics of extracellular polymeric substances(EPS) by activated sludge using weighted nonlinear least-squares analysis[J]. Environmental Science & Technology, 2009,43:3743-3750.