Abstract: The acceleration of the urbanization process and a series of unreasonable development and utilization of wetland resources have caused serious damage of the wetland, and the monitoring of the temporal and spatial changes of wetland resources plays important role in the regional development and sustainable development of wetland. Based on multi-period land use data and long-term sequence Landsat image data, the loss status of wetland types were analyzed by using Markov transfer matrix and GIS spatial analysis method. The trend analysis method was used to analyze the wetland vegetation, water body and soil moisture elements loss in long-term sequences and different stages. The remote sensing identification method of wetland loss was constructed, and the wetland loss patterns were summarized combined with wetland type and wetland element loss, with a case study in Tianjin Binhai New Area. The results showed that the damage of wetland types in Binhai New Area was mainly divided into three periods (1980s-2000, 2000-2009, 2009-2015). From 1980s to 2015, the wetland experienced the process from damage to recovery, and the wetland area increased by 41.40 km ². Wetland elements showed a degradation trend from 1984 to 2015. The areas of significant degradation of NDVI, NDWI and SMMI were 364.66, 221.28 and 253.94 km ², respectively. In three different periods, different dominant factors affected the damaged area of wetland. In Binhai New Area, the wetland loss was mainly caused by vegetation, water area and vegetation during the three periods.