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微生物降解污染地下水中三氯乙烯的微宇宙试验研究

孙仲平 吴乃瑾 杨苏才 魏文侠 宋云

孙仲平, 吴乃瑾, 杨苏才, 魏文侠, 宋云. 微生物降解污染地下水中三氯乙烯的微宇宙试验研究[J]. 环境工程技术学报, 2021, 11(2): 298-306. doi: 10.12153/j.issn.1674-991X.20200150
引用本文: 孙仲平, 吴乃瑾, 杨苏才, 魏文侠, 宋云. 微生物降解污染地下水中三氯乙烯的微宇宙试验研究[J]. 环境工程技术学报, 2021, 11(2): 298-306. doi: 10.12153/j.issn.1674-991X.20200150
SUN Zhongping, WU Naijin, YANG Sucai, WEI Wenxia, SONG Yun. Microcosm experimental study on microbial degradation of trichloroethylene in contaminated groundwater[J]. Journal of Environmental Engineering Technology, 2021, 11(2): 298-306. doi: 10.12153/j.issn.1674-991X.20200150
Citation: SUN Zhongping, WU Naijin, YANG Sucai, WEI Wenxia, SONG Yun. Microcosm experimental study on microbial degradation of trichloroethylene in contaminated groundwater[J]. Journal of Environmental Engineering Technology, 2021, 11(2): 298-306. doi: 10.12153/j.issn.1674-991X.20200150

微生物降解污染地下水中三氯乙烯的微宇宙试验研究

doi: 10.12153/j.issn.1674-991X.20200150
详细信息
    作者简介:

    孙仲平(1994—),男,硕士,主要研究方向为场地污染调查与修复, s5566163@163.com

    通讯作者:

    宋云 E-mail: liepi_song@163.com

  • 中图分类号: X523

Microcosm experimental study on microbial degradation of trichloroethylene in contaminated groundwater

More Information
    Corresponding author: SONG Yun E-mail: liepi_song@163.com
  • 摘要: 三氯乙烯(TCT)是我国地下水中一类检出率极高的挥发性氯代烃有机物,为丰富国内氯代烃污染地下水生物修复的理论支持,从北京市某氯代烃实际污染场地采集含水层沉积物及地下水,利用微宇宙试验体系,在厌氧条件下分别研究了添加不同浓度的醋酸钠、乳酸钠、乳酸对地下水中TCE去除效果的影响,结合各厌氧体系内中间产物的分析和微生物多样性的变化对反应机理进行阐述。结果表明:厌氧条件下,添加1.0 g/L醋酸钠的反应体系中TCE的去除率最高,可达94.5%,且添加醋酸钠的反应体系可长时间维持中性pH及较低的氧化还原电位。在对厌氧反应降解中间产物的分析中,反应第30天中间产物只检测到了顺式1,2-二氯乙烯,推测本试验厌氧条件下TCE生物降解主要机制为氢解反应;微宇宙体系内,各样品的优势菌门均含有Proteobacteria(变形菌门)、Firmicutes(厚壁菌门),且均为氯代烃的潜在高效降解菌;基因定量分析显示,各反应体系中细菌总量显著增长,且各样品均检测到较高水平的功能基因tceA(水样中拷贝数可达106~107 L-1),推测TCE的降解可能是在功能基因tceA的作用下而发生氢解反应。

     

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  • 收稿日期:  2020-06-12
  • 刊出日期:  2021-03-20

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