微生物降解污染地下水中三氯乙烯的微宇宙试验研究

孙仲平; 吴乃瑾; 杨苏才; 魏文侠; 宋云

doi:10.12153/j.issn.1674-991X.20200150

微生物降解污染地下水中三氯乙烯的微宇宙试验研究

doi: 10.12153/j.issn.1674-991X.20200150

轻工业环境保护研究所,工业场地污染与修复北京市重点实验室

详细信息

作者简介:
孙仲平(1994—),男,硕士,主要研究方向为场地污染调查与修复, s5566163@163.com

通讯作者:
宋云 E-mail: liepi_song@163.com

中图分类号: X523
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出版历程
- 收稿日期: 2020-06-12
- 刊出日期: 2021-03-20

Microcosm experimental study on microbial degradation of trichloroethylene in contaminated groundwater

Beijing Key Laboratory of Industrial Land Contamination and Remediation,Environmental Protection Research Institute of Light Industry

More Information

Corresponding author: SONG Yun E-mail: liepi_song@163.com

摘要

摘要: 三氯乙烯(TCT)是我国地下水中一类检出率极高的挥发性氯代烃有机物,为丰富国内氯代烃污染地下水生物修复的理论支持,从北京市某氯代烃实际污染场地采集含水层沉积物及地下水,利用微宇宙试验体系,在厌氧条件下分别研究了添加不同浓度的醋酸钠、乳酸钠、乳酸对地下水中TCE去除效果的影响,结合各厌氧体系内中间产物的分析和微生物多样性的变化对反应机理进行阐述。结果表明:厌氧条件下,添加1.0 g/L醋酸钠的反应体系中TCE的去除率最高,可达94.5%,且添加醋酸钠的反应体系可长时间维持中性pH及较低的氧化还原电位。在对厌氧反应降解中间产物的分析中,反应第30天中间产物只检测到了顺式1,2-二氯乙烯,推测本试验厌氧条件下TCE生物降解主要机制为氢解反应;微宇宙体系内,各样品的优势菌门均含有Proteobacteria(变形菌门)、Firmicutes(厚壁菌门),且均为氯代烃的潜在高效降解菌;基因定量分析显示,各反应体系中细菌总量显著增长,且各样品均检测到较高水平的功能基因tceA(水样中拷贝数可达10⁶~10⁷ L^-1),推测TCE的降解可能是在功能基因tceA的作用下而发生氢解反应。
- 三氯乙烯 /
- 地下水 /
- 生物修复 /
- 厌氧 /
- 微生物多样性
Abstract: Volatile chlorinated hydrocarbons such as trichloroethylene have become one of the most highly detected organic pollutants in groundwater of China. In order to enrich the theoretical support of bioremediation of groundwater polluted by chlorinated hydrocarbons in China, the aquifer sediments and groundwater samples were collected from a chlorinated hydrocarbons contaminated site in Beijing. The effects of different concentrations of sodium acetate, sodium lactate and lactic acid on the removal of trichloroethylene under anaerobic conditions were preliminarily investigated by the microcosm experiments. Combined with the analysis of the intermediate products and the change of microbial diversity in each anaerobic system, the reaction mechanism was elaborated. The results showed that under the anaerobic condition, the removal rate of trichloroethylene in the system of 1.0 g/L sodium acetate was the highest, and the degradation rate was 94.5%. The test system with sodium acetate could maintain neutral pH and low redox potential for a long time, and at the same time, it could achieve the highest TOC degradation rate. Only cis-1,2-dichloroethylene was detected in the intermediates of anaerobic degradation on the 30th day of the reaction. It was inferred that the main mechanism of biodegradation of trichloroethylene under the anaerobic condition was hydrogenolysis. In the microcosm system, the dominant microbes at the phylum level contained Proteobacteriae and Firmicutes, which were potential high-efficiency biodegradable bacteria for chlorinated hydrocarbons. The qPCR results showed that the total amount of bacteria increased greatly in each reaction system, and a high level of tceA was detected in each sample (about 10⁶-10⁷copies/L), suggesting that the hydrogenolysis of TCE might be carried out under the function of tceA.
- trichloroethylene /
- groundwater /
- bioremediation /
- anaerobic /
- microbial diversity

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