不同填埋龄垃圾腐殖土中细菌群落结构特征

摘要: 为探究填埋龄对垃圾腐殖土内细菌群落结构的影响,以我国江苏省某生活垃圾填埋场中腐殖土为研究对象,采用Illumina Miseq高通量测序技术分析了填埋年限分别为1~3、3~6、6~10 a的腐殖土及填埋场周边土壤中的细菌群落组成。结果表明:腐殖土中细菌群落的丰富度和多样性随填埋年限呈增加趋势,但均低于填埋场周边土壤。非度量多维尺度(NMDS)分析结果表明,不同填埋年限的腐殖土中细菌群落结构差异性较大,但填埋场周边土壤中细菌群落与填埋年限为6~10 a的腐殖土相似度较高,可能是周边土壤在一定程度上受到了垃圾渗滤液的污染。厚壁菌门、变形菌门、放线菌门、绿弯菌门和拟杆菌门均为各阶段腐殖土中的优势菌门,厚壁菌门的相对丰度随填埋年限增加先增加后减少,绿弯菌门则先减少后增加,拟杆菌门的相对丰度在填埋后期出现下降,而变形菌门和放线菌门在整个过程中无明显变化。冗余分析(RDA)结果表明,腐殖土中细菌群落结构的变化与总氮(TN)、重金属(Cd、Zn)及pH密切相关。
- 垃圾填埋场 /
- 填埋龄 /
- 腐殖土 /
- 细菌群落
Abstract: In order to explore the effect of landfill time on the bacterial community structure of humus soil, taking humus in a domestic waste landfill in Jiangsu Province, China as the research object, Illumina Miseq high-throughput sequencing technology was used to analyze the bacterial community composition of humus and the surrounding soil with landfill time of 1-3 years, 3-6 years and 6-10 years. The results showed that the richness and diversity of the bacterial community in humus soil increased with the landfill time, but they were lower than those in the surrounding soil. Non metric multidimensional scaling (NMDS) analysis showed that the bacterial community structure difference was bigger in humus soil of different landfill time, but the similarity between the bacterial community in the soil surrounding the landfill site and the humus soil buried for 6-10 years was relatively high, which may be caused by the surrounding soil being polluted by landfill leachate to some extent. Firmicutes, Proteobacteria, Actinobacteriota, Chloroflexi and Bacteroidota were the dominant phylum in humus soil at each stage. The relative abundance of Firmicutes increased first and then decreased with the landfill time, the change of Chloroflexi was the opposite. The relative abundance of Bacteroidetes decreased at the later stage of landfill, while the relative abundance of Proteobacteria and Actinobacteria did not change significantly during the whole process. Redundant analysis (RDA) results showed that the change of bacterial community structure in humus soil was closely related to total nitrogen (TN), heavy metals (Cd, Zn) and pH.
- landfill /
- landfill age /
- humus soil /
- bacterial community

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