进水方式对处理分散养猪冲洗废水ABR反应器微生物群落结构的影响

董婧; 卢少奇; 伍娟丽; 肖宇; 沈志强; 周岳溪

doi:10.12153/j.issn.1674-991X.20210148

进水方式对处理分散养猪冲洗废水ABR反应器微生物群落结构的影响

doi: 10.12153/j.issn.1674-991X.20210148

董婧^{1, 2,},
卢少奇^{1, 2},
伍娟丽^{1, 2},
肖宇³,
沈志强³,
周岳溪^3, ,

1.
北京市环境保护科学研究院
2.
国家城市环境污染控制工程技术研究中心
3.
中国环境科学研究院环境污染控制工程技术研究中心

基金项目: 北京市环境保护科学研究院院基金（Y2010-010）；北京市环境保护科学研究院纵向结余项目（JZ2020-005）；“十二五”国家科技支撑计划项目（2012BAJ21B01-02）

详细信息

作者简介:
董婧(1984—)，女，高级工程师，博士，主要从事环境污染治理技术研究，dongjing1098@sina.com

通讯作者:
周岳溪（1963—），男，研究员，博士，长期从事水污染控制技术研究，zhouyuexi@263.net

中图分类号: X703
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- 被引次数: 0
出版历程
- 收稿日期: 2021-04-22
- 网络出版日期: 2022-06-07

Effects of influent mode on the microbial community structure in anaerobic baffled reactor during the treatment of dispersed swine flushing wastewater

DONG Jing^{1, 2
,},
LU Shaoqi^{1, 2},
WU Juanli^{1, 2},
XIAO Yu³,
SHEN Zhiqiang³,
ZHOU Yuexi^{3
, ,}

1.
Beijing Municipal Research Institute of Environmental Protection
2.
National Engineering Research Center for Urban Environmental Pollution Control
3.
Research Center of Environmental Pollution Control Engineering Technology, Chinese Research Academy of Environmental Sciences

摘要

摘要:
以厌氧折流板反应器（ABR）处理分散养猪冲洗废水，对比平行运行的序批进水和连续进水ABR反应器在不同进水负荷（3个运行阶段）下的微生物群落结构变化及差异。结果表明：主坐标分析（PCoA）和聚类分析均证实，相同进水负荷时，连续进水和序批进水ABR装置的微生物群落结构整体上较接近。进水方式对微生物群落结构的影响主要在ABR的第一格（也是COD的主要去除区域）。第三阶段，连续进水装置丰度排名前5的属为Clostridium_sensu_stricto_1、Norank_f_Anaerolineaceae、Christensenellaceae_R-7_group、Norank_c_Bacteroidetes_vadinHA17和Norank_p_Candidate_division_WS6，其相对丰度分别为9.04%、8.14%、7.08%、5.97%和4.88%；序批进水装置丰度排名前5的属为Norank_c_Bacteroidetes_vadinHA17、Clostridium_sensu_stricto_1、Christensenellaceae_R-7_group、Norank_f_Anaerolineaceae和Desulfocapsa，其相对丰度分别为10.03%、7.38%、6.92%、5.58%和4.29%。费舍尔精确检验证实，第三阶段序批进水和连续进水 ABR 反应器的15种丰度较高的微生物中，13种有显著性差异，其中12种P≤0.001。
- 厌氧折流板反应器 /
- 分散养猪冲洗废水 /
- 微生物群落 /
- 进水方式 /
- 费舍尔精确检验
Abstract:
The treatment of flushing wastewater from scattered piggeries by anaerobic baffled reactor (ABR) was explored. The variations and differences of the microbial communities in the ABRs respectively with the influent mode-sequencing batch and the continuous operation under different pollution loads （3 operational phases) were compared. The results showed that, according to PCoA and cluster analysis, the microbial communities between the sequencing batch and continuous ABRs were similar under the same pollution load. The influence of the influent mode on microbial community structure was mainly presented in the first compartment (also the main removal area of COD). The five dominant genera in the continuous operation ABR were Clostridium_sensu_stricto_1, Norank_f_Anaerolineaceae, Christensenellaceae_R-7_group, Norank_c_Bacteroidetes_vadinHA17 and Norank_p_Candidate_division_WS6, with the relative abundance of 9.04%, 8.14%, 7.08%, 5.97% and 4.88%, respectively. While the five dominant genera in the sequencing batch ABR were Norank_c_Bacteroidetes_vadinHA17, Clostridium_sensu_stricto_1, Christensenellaceae_R-7_group, Norank_f_Anaerolineaceae and Desulfocapsa, with the relative abundance of 10.03%, 7.38%, 6.92%, 5.58% and 4.29%, respectively. Fisher’ exact test verified that there were 13 genera showing significant difference among the top 15 genera with high abundance in the two ABRs in the third stage, and the difference of 12 genera showed extremely difference (P≤0.001).
- anaerobic baffled reactor /
- scattered piggery flushing water /
- microbial community /
- influent mode /
- Fisher’ exact test

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图 1 ABR装置流程

Figure 1. Process of the anaerobic baffled reactor (ABR)

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图 2 装置A和装置B 3个运行阶段样品基于Bray-Curtis距离矩阵的主坐标分析(PCoA)

Figure 2. PCoA based on Bray-Curtis distance matrix for the samples of Reactors A and B at three operation phases

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图 3 装置A和B 3个运行阶段的样品层级聚类分析

Figure 3. Hierarchical clustering analysis of Reactors A and B at three operation phases on sample level

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图 4 装置A和装置B第一格细菌在门水平的相对丰度

Figure 4. Relative abundance of bacteria in the first compartment of Reactors A and B on the phylum level

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图 5 装置A和装置B第一格细菌在属水平的相对丰度

Figure 5. Relative abundance of bacteria in the first case of Reactors A and B on the genus level

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图 6 3个阶段装置A和装置B样品费舍尔精确检验显著性差异

Figure 6. Significant difference of Fisher’exact test between Reactors A and B samples at three phases

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图 7 第一阶段装置A和装置B第一格样品费舍尔精确检验显著性差异

Figure 7. Significant difference of Fisher’exact test between Reactors A and B samples in the first compartment at the first phase

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图 8 第二阶段装置A和装置B第一格样品费舍尔精确检验显著性差异

Figure 8. Significant difference of Fisher’exact test between Reactors A and B samples in the first case at the second phase

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图 9 第三阶段装置A和装置B第一格样品费舍尔精确检验显著性差异

Figure 9. Significant difference of Fisher’exact test between Reactors A and B samples in the first case at the third phase

下载: 全尺寸图片幻灯片

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