Differential responses of five wetland plants and their root microbial communities under ofloxacin pollution stress
-
摘要:
人工湿地中植物及其根系微生物对环境中的抗生素具有较好的去除效果，但对抗生素胁迫下多种植物及微生物响应特征的了解仍然不足，因此有必要研究不同浓度抗生素胁迫下植物及微生物的差异性响应特征。探讨了典型极性抗生素氧氟沙星（OFL）胁迫下5种湿地植物（唐菖蒲、风车草、水葱、水芹、灯心草）-微生物系统对OFL的去除性能。基于不同植物对OFL的去除效率，对唐菖蒲和风车草的根系活力、活性氧、抗氧化系统进行研究，同时分析其根系微生物在OFL胁迫下的响应特征。结果表明：5种湿地植物对OFL的去除效果具有明显差异性，其中风车草对OFL的去除性能最佳，其次是唐菖蒲。植物-微生物系统在不同浓度OFL胁迫下表现出不同敏感性，以10 mg/L为转折点，当OFL浓度低于10 mg/L时，可促进唐菖蒲和风车草植物根系生长，增强植物根系活力以及根系抗氧化酶的活性；当OFL浓度超过10 mg/L时，唐菖蒲和风车草根系被OFL毒害，植物根系活力和抗氧化酶的活性受到抑制。利用高通量测序技术对根系微生物群落进行测定，结果表明，低浓度OFL（1 mg/L）与植物根系微生物群落多样性及物种丰富度呈正相关，高浓度（50 mg/L）下则呈负相关；利用PICRUSt功能软件对微生物群落功能进行预测，发现高浓度OFL胁迫可加快植物根系微生物的代谢速度。
Abstract:The plants and their root microorganisms in the constructed wetland have a good removal effect on the antibiotics in the environment, but the understanding of the response characteristics of various plants and microorganisms under antibiotic stress is still insufficient and unclear, so it is necessary to clearly study the differential response characteristics of plants and microorganisms under different concentrations of antibiotic stress. The removal performance of ofloxacin (OFL) by five wetland plants (Gladiolus hybrids, Cyperus alternifolius, Scirpus validus Vahl, Oenanthe javanica, Juncus effusus L.) microbial systems under the stress of OFL, a typical polar antibiotic, was studied. Based on the removal efficiency of OFL by different plants, the root activity, active oxygen and antioxidant system of Gladiolus hybrids and Cyperus alternifolius were studied, and the response characteristics of their root microorganisms under OFL stress were analyzed. The results showed that the five wetland plants had significant differences in the removal of OFL, among which the Cyperus alternifolius had the best removal performance of OFL, followed by Gladiolus hybrids. The plant-microbial systems showed different sensitivities under OFL stress of different concentrations. Taking 10 mg/L as the turning point, when OFL concentration was below 10 mg/L, it could promote plant root growth, plant root activity and root antioxidant enzyme activity. When OFL concentration exceeded 10 mg/L, Gladiolus hybrids and Cyperus alternifolius roots were poisoned by OFL, and plant root activity and antioxidant enzyme activity were inhibited. The measurement results of root microbial community using high-throughput sequencing technology showed that low concentration OFL (1 mg/L) was positively correlated with plant root microbial community diversity and species richness, while high concentration OFL (50 mg/L) was negatively correlated. Using PICRUSt function software to predict the function of microbial community, it was found that high concentration OFL stress could accelerate the metabolic rate of plant root microorganisms.
-
Key words:
- wetland plants /
- ofloxacin (OFL) /
- plant activity /
- microbial community diversity
-
图 1 5种植物对不同初始浓度OFL的去除率
注：同一时间字母不同表示处理组间有显著性差异（P<0.05），下同。
Figure 1. Removal rate of OFL with different initial concentrations by five plants
图 2 2种植物在不同浓度OFL胁迫下根系活力的变化
Figure 2. Changes of root activity of two plants under OFL stress of different concentrations
图 3 2种植物在不同浓度OFL胁迫下根系活性氧自由基含量和抗氧化酶活性的变化
Figure 3. Changes of ROS free radical content and antioxidant enzyme activity in roots of two plants under OFL stress of different concentrations
图 4 门水平下2种植物根系微生物群落的相对丰度占比
Figure 4. Relative abundance of root microbial communities of two plants at phylum levels
图 5 属水平下2 种植物根系微生物群落的相对丰度
注：A代表唐菖蒲；B代表风车草；CK代表对照组；F1、F50分别代表OFL浓度为1和50 mg/L。
Figure 5. Relative abundance of root microbial communities of two plants at genus levels
图 6 2种植物根系微生物群落代谢功能基因丰度
注：字母与数字含义同图5。
Figure 6. Abundance of metabolic function genes in root microbial communities of two plants
表 1 2种植物根系微生物群落的多样性指数分析
Table 1. Diversity index analysis of root microbial communities of two plants
OFL浓度/
（mg/L）植物 Shannon
指数Simpson
指数Ace
指数Chao
指数样品覆
盖率/%0 唐菖蒲 4.30 0.03 565.31 568.51 99.58 风车草 4.33 0.02 533.39 549.67 98.88 1 唐菖蒲 4.62 0.02 582.78 609.56 99.62 风车草 4.64 0.02 576.93 565.5 99.52 50 唐菖蒲 2.04 0.25 556.57 498.81 99.85 风车草 3.46 0.07 368.52 384.76 99.39 
下载: 导出CSV
-
[1] HARUHIKO N, KURUNTHACHALAM K, PAUL D J, et al. Determination of fluoroquinolone antibiotics in wastewater effluents by liquid chromatography mass spectrometry and fluorescence detection[J]. Chemosphere,2005,58:759-766. doi: 10.1016/j.chemosphere.2004.08.097 [2] 许若梦, 吴桐, 锁瑞娟,等.基于不同自由基的高级氧化技术对水中诺氟沙星的去除效果[J]. 环境工程技术学报,2020,10(3):433-439. doi: 10.12153/j.issn.1674-991X.20190177XU R M, WU T, SUO R J,et al. Removal performance of norfloxacin from waters by advanced oxidation processes based on different free radicals[J]. Journal of Environmental Engineering Technology,2020,10(3):433-439. doi: 10.12153/j.issn.1674-991X.20190177 [3] ZHANG Q Q, YING G G, PAN C G, et al. Comprehensive evaluation of antibiotics emission and fate in the river basins of China: source analysis, multimedia modeling, and linkage to bacterial resistance[J]. Environmental Science & Technology,2015,49(11):6772-6782. [4] LI Y X, ZHANG X L, LI W, et al. The residues and environmental risks of multiple veterinary antibiotics in animal faeces[J]. Environmental Monitoring and Assessment,2013,185(3):2211-2220. doi: 10.1007/s10661-012-2702-1 [5] XIE W Y, YANG X P, LI Q, et al. Changes in antibiotic concentrations and antibiotic resistome during commercial composting of animal manures[J]. Environmental Pollution,2016,219:182-190. doi: 10.1016/j.envpol.2016.10.044 [6] ZHOU L J, YING G G, ZHAO J L, et al. Trends in the occurrence of human and veterinary antibiotics in the sediments of the Yellow River, Hai River and Liao River in Northern China[J]. Environmental Pollution,2011,159(7):1877-1885. doi: 10.1016/j.envpol.2011.03.034 [7] GRENNI P, ANCONA V, BARRA CARACCIOLO A. Ecological effects of antibiotics on natural ecosystems: a review[J]. Microchemical Journal,2018,136:25-39. doi: 10.1016/j.microc.2017.02.006 [8] YANG Y Y, SONG W J, LIN H, et al. Antibiotics and antibiotic resistance genes in global lakes: a review and meta-analysis[J]. Environment International,2018,116:60-73. doi: 10.1016/j.envint.2018.04.011 [9] LI S, SHI W Z, LIU W, et al. A duodecennial national synthesis of antibiotics in China's major rivers and seas (2005-2016)[J]. Science of the Total Environment,2018,615:906-917. doi: 10.1016/j.scitotenv.2017.09.328 [10] CHEN J, YING G G, WEI X D, et al. Removal of antibiotics and antibiotic resistance genes from domestic sewage by constructed wetlands: effect of flow configuration and plant species[J]. Science of the Total Environment,2016,571:974-982. doi: 10.1016/j.scitotenv.2016.07.085 [11] MILLER E L, NASON S L, KARTHIKEYAN K G, et al. Root uptake of pharmaceuticals and personal care product ingredients[J]. Environmental Science & Technology,2016,50(2):525-541. [12] LIU L, LIU Y H, LIU C X, et al. Potential effect and accumulation of veterinary antibiotics in Phragmites australis under hydroponic conditions[J]. Ecological Engineering,2013,53:138-143. doi: 10.1016/j.ecoleng.2012.12.033 [13] GIBSON M K, FORSBERG K J, DANTAS G. Improved annotation of antibiotic resistance determinants reveals microbial resistomes cluster by ecology[J]. The ISME Journal,2015,9(1):207-216. doi: 10.1038/ismej.2014.106 [14] CABELLO F C. Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment[J]. Environmental Microbiology,2006,8(7):1137-1144. doi: 10.1111/j.1462-2920.2006.01054.x [15] GOMES M P, le MANAC'H S G, MOINGT M, et al. Impact of phosphate on glyphosate uptake and toxicity in willow[J]. Journal of Hazardous Materials,2016,304:269-279. doi: 10.1016/j.jhazmat.2015.10.043 [16] ZHANG Z H, LIU X N, LÜ Y, et al. Grafting resulting in alleviating tomato plant oxidative damage caused by high levels of ofloxacin[J]. Environmental Pollution,2021,286:117331. doi: 10.1016/j.envpol.2021.117331 [17] MAN Y, WANG J X, TAM N F Y, et al. Responses of rhizosphere and bulk substrate microbiome to wastewater-borne sulfonamides in constructed wetlands with different plant species[J]. Science of the Total Environment,2020,706:135955. doi: 10.1016/j.scitotenv.2019.135955 [18] XIONG W G, SUN Y X, ZHANG T, et al. Antibiotics, antibiotic resistance genes, and bacterial community composition in fresh water aquaculture environment in China[J]. Microbial Ecology,2015,70(2):425-432. doi: 10.1007/s00248-015-0583-x [19] LÜ Y, LI Y Y, LIU X H, et al. The tolerance mechanism and accumulation characteristics of Phragmites australis to sulfamethoxazole and ofloxacin[J]. Chemosphere,2020,253:126695. doi: 10.1016/j.chemosphere.2020.126695 [20] 汤叶涛, 关丽捷, 仇荣亮, 等.镉对超富集植物滇苦菜抗氧化系统的影响[J]. 生态学报,2010(2):324-332.TANG Y T, GUAN L J, QIU R L, et al. Antioxidative defense to cadmium in hyperaccumulator Picris divaricata V[J]. Acta Ecologica Sinica,2010(2):324-332. [21] GIANNOPOLITIS C N, RIES S K. Superoxide dismutases:Ⅱ. purification and quantitative relationship with water-soluble protein in seedlings[J]. Plant Physiology,1977,59(2):315-318. doi: 10.1104/pp.59.2.315 [22] LIU N, LIN Z F, GUAN L L, et al. Antioxidant enzymes regulate reactive oxygen species during pod elongation in Pisum sativum and Brassica chinensis[J]. PLoS One,2014,9(2):e87588. doi: 10.1371/journal.pone.0087588 [23] HU X G, ZHOU Q X, LUO Y. Occurrence and source analysis of typical veterinary antibiotics in manure, soil, vegetables and groundwater from organic vegetable bases, Northern China[J]. Environmental Pollution,2010,158(9):2992-2998. doi: 10.1016/j.envpol.2010.05.023 [24] LÜ Y, XU J M, XU K, et al. Accumulation characteristics and biological response of ginger to sulfamethoxazole and ofloxacin[J]. Environmental Pollution,2020,262:114203. doi: 10.1016/j.envpol.2020.114203 [25] 周品成, 刘希强, 康兴生, 等.4种水生植物对兽用抗生素去除效果比较[J]. 华南农业大学学报,2019,40(6):67-73. doi: 10.7671/j.issn.1001-411X.201901020ZHOU P C, LIU X Q, KANG X S, et al. Removal effects of four aquatic plants on veterinary antibiotics[J]. Journal of South China Agricultural University,2019,40(6):67-73. doi: 10.7671/j.issn.1001-411X.201901020 [26] 杨斌, 张文辉, 高建欣.5种速生柳无性系对Pb2+胁迫的生理抗性比较[J]. 西北植物学报,2015,35(6):1182-1189. doi: 10.7606/j.issn.1000-4025.2015.06.1182YANG B, ZHANG W H, GAO J X. Physiological resistance of five fast-growing willow clones to lead (Pb) stress[J]. Acta Botanica Boreali-Occidentalia Sinica,2015,35(6):1182-1189. doi: 10.7606/j.issn.1000-4025.2015.06.1182 [27] 廖德润, 刘超翔, 王振, 等.兽用抗生素胁迫对空心菜的影响研究[J]. 环境科学学报,2013,33(9):2558-2564. doi: 10.13671/j.hjkxxb.2013.09.008LIAO D R, LIU C X, WANG Z, et al. Effects of veterinary antibiotics stress on Ipomoea aquatica Forsk[J]. Acta Scientiae Circumstantiae,2013,33(9):2558-2564. doi: 10.13671/j.hjkxxb.2013.09.008 [28] 鲍陈燕, 顾国平, 章明奎.兽用抗生素胁迫对水芹生长及其抗生素积累的影响[J]. 土壤通报,2016(1):164-172. doi: 10.19336/j.cnki.trtb.2016.01.026BAO C Y, GU G P, ZHANG M K. Effects of veterinary antibiotics stress on growth and antibiotics accumulation of Oenanthe javanica DC[J]. Chinese Journal of Soil Science,2016(1):164-172. doi: 10.19336/j.cnki.trtb.2016.01.026 [29] ZHANG T Y, YU B B, LIN W X, et al. Effects of sulfadimidine and ciprofloxacin stress on seed germination and seedling growth of wheat[J]. Journal of Agricultural Resources and Environment,2021,38(2):176-184. [30] WEBER K P, MITZEL M R, SLAWSON R M, et al. Effect of ciprofloxacin on microbiological development in wetland mesocosms[J]. Water Research,2011,45(10):3185-3196. doi: 10.1016/j.watres.2011.03.042 [31] ZHANG H, LI X, YANG Q, et al. Plant growth, antibiotic uptake, and prevalence of antibiotic resistance in an endophytic system of pakchoi under antibiotic exposure[J]. International Journal of Environmental Research and Public Health,2017,14(11):1336. doi: 10.3390/ijerph14111336 [32] CALABRESE E J, BLAIN R B. Hormesis and plant biology[J]. Environmental Pollution,2009,157(1):42-48. doi: 10.1016/j.envpol.2008.07.028 [33] RIAZ L, MAHMOOD T, COYNE M S, et al. Physiological and antioxidant response of wheat (Triticum aestivum) seedlings to fluoroquinolone antibiotics[J]. Chemosphere,2017,177:250-257. doi: 10.1016/j.chemosphere.2017.03.033 [34] MIGLIORE L, COZZOLINO S, FIORI M. Phytotoxicity to and uptake of enrofloxacin in crop plants[J]. Chemosphere,2003,52(7):1233-1244. doi: 10.1016/S0045-6535(03)00272-8 [35] LI J B, LUO C L, ZHANG D Y, et al. Diversity of the active phenanthrene degraders in PAH-polluted soil is shaped by ryegrass rhizosphere and root exudates[J]. Soil Biology and Biochemistry,2019,128:100-110. doi: 10.1016/j.soilbio.2018.10.008 [36] 张崇淼, 徐欢, 刘静, 等.城市污水处理系统中沙门氏菌对四环素和磺胺甲恶唑的耐药性[J]. 环境科学研究,2014(3):309-313. doi: 10.13198/j.issn.1001-6929.2014.03.13ZHANG C M, XU H, LIU J, et al. Tetracycline and sulfamethoxazole resistance and distribution of resistance genes in Salmonella isolated from municipal wastewater treatment plants[J]. Research of Environmental Sciences,2014(3):309-313. doi: 10.13198/j.issn.1001-6929.2014.03.13 [37] XU J M, LIU X H, LV Y, et al. Response of Cyperus involucratus to sulfamethoxazole and ofloxacin-contaminated environments: growth physiology, transportation, and microbial community[J]. Ecotoxicology and Environmental Safety,2020,206:111332. doi: 10.1016/j.ecoenv.2020.111332 [38] LIU X, LI X Z, ONG S M C, et al. Progress of phytoremediation: focus on new plant and molecular mechanism[J]. Plant Biology & Soil Health,2013,1(1):5-9. [39] HUERTA B, MARTI E, GROS M, et al. Exploring the links between antibiotic occurrence, antibiotic resistance, and bacterial communities in water supply reservoirs[J]. Science of the Total Environment,2013,456/457:161-170. doi: 10.1016/j.scitotenv.2013.03.071 [40] FANG D X, ZHAO G, XU X Y, et al. Microbial community structures and functions of wastewater treatment systems in plateau and cold regions[J]. Bioresource Technology,2018,249:684-693. doi: 10.1016/j.biortech.2017.10.063 [41] HOEFEL D, MONIS P T, GROOBY W L, et al. Profiling bacterial survival through a water treatment process and subsequent distribution system[J]. Journal of Applied Microbiology,2005,99(1):175-186. doi: 10.1111/j.1365-2672.2005.02573.x [42] WANG G, PARK J, SANDHU R, et al. Dynamic trust evaluation model based on bidding and multi-attributes for social networks[J]. International Journal of High Performance Computing and Networking,2019,13(4):436. doi: 10.1504/IJHPCN.2019.099267 [43] 张颖. 黄瓜低温胁迫应答转录因子CsWRKY46和CsWRKY21的表达特征与功能分析[D]. 北京: 中国农业科学院, 2012. [44] WANG G, WANG Y X, JI F, et al. Biodegradation of deoxynivalenol and its derivatives by Devosia insulae A16[J]. Food Chemistry,2019,276:436-442. ⊗ doi: 10.1016/j.foodchem.2018.10.011 -
下载:
点击查看大图
计量
- 文章访问数: 293
- HTML全文浏览量: 146
- PDF下载量: 26
- 被引次数: 0
