Abstract: Port planning environmental impact assessment (EIA) has played an important role in promoting port space resource intensive conservation and ecological protection. However, the existing port planning EIA index system lacks adaptability and relevance in responding to new policies and requirements for ecological environment protection. Based on the existing research results of EIA index system, the port planning EIA index system under the new situations was constructed using the basic index system model according to the environmental impact characteristics of the port master plans. The indicator system contained 4 categories, including ecological protection, environmental quality protection, resource utilization and environmental risk and emergency response, and a total of 50 indicators in 18 aspects, including ecological conservation redline, ecological environment subarea control, environmental sensitive area, shoreline zoning, ecological environment, fishery resources, biodiversity, water environment, atmospheric environment, carbon emission, sound environment, shoreline resources, land resources, water resources, resource recycling, berth specialization level, environmental risk, and emergency response. The relevant indicators generally reflected the environmental protection requirements, the degree of environmental impact and the level of resource utilization corresponding to the implementation of the plan, which could provide reference for port planning EIA practice.