Inhibition and removal characteristics of trichloroethylene on anaerobic hydrolysis acidifying bacteria
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摘要:
三氯乙烯（TCE）是石化废水中典型的有机污染物，对微生物具有极强的毒性。通过对挥发性脂肪酸批次试验进行生物测定，探讨TCE对厌氧水解酸化菌的产酸抑制作用，在TCE作用下水解酸化菌的胞外聚合物（EPS）和污泥zeta电位的变化以及TCE的去除特性。结果表明：TCE浓度为75 mg/L（半抑制浓度，EC50）时，对水解酸化菌的产酸量有抑制作用；随着TCE浓度升高，水解酸化菌的EPS中蛋白质浓度先增大后减少，其中TCE浓度为50 mg/L时EPS中蛋白质浓度达到最大值，为（33.94±0.25）mg/L；zeta电位的结果显示，污泥的凝聚性能随TCE浓度增大（0~100 mg/L）而增大；厌氧水解酸化菌对TCE的脱氯能力随TCE浓度的升高而降低，水解酸化菌转化TCE的脱氯率由TCE浓度为10 mg/L时的77.83%降为200 mg/L时的6.67%。TCE对水解酸化菌具有强烈的抑制作用，TCE主要是通过抑制细胞的蛋白质合成来抑制微生物活性，进而限制水解酸化菌降解TCE的能力。
Abstract:Trichloroethylene (TCE) is a typical organic pollutant in petrochemical wastewater, which is highly toxic to microorganisms. Batch bioassays of volatile fatty acid were carried out to explore the inhibitory effect of TCE on acid production of anaerobic hydrolysis acidifying bacteria, the variation of extracellular polymeric substances (EPS) and mud zeta potential of hydrolysis acidifying bacteria under TCE shock, and the removal characteristics of TCE. The results showed that TCE at a concentration of 75 mg/L (semi-inhibitory concentration, EC50) had an inhibitory effect on the acid production of hydrolysis acidifying bacteria. With the increase of TCE concentration, the protein concentration in EPS of hydrolsis acidifying bacteria first increased and then decreased. The maximum value of protein concentration in EPS was (33.94±0.25)mg/L when TCE concentration was 50 mg/L. The results of zeta potential showed that the coagulation performance of sludge increased with the increase of TCE concentration (0-100 mg/L). The dechlorination ability of anaerobic hydrolysis acidifying bacteria to TCE decreased with the increase of TCE concentration, and the dechlorination rate of TCE converted by hydrolysis acidifying bacteria was 77.83% when the concentration of TCE was 10 mg/L. It decreased to 6.67% at 200 mg/L. TCE had a strong inhibitory effect on hydrolysis acidifying bacteria. TCE mainly inhibited microbial activity by inhibiting the protein synthesis of cells, thereby limiting the ability of hydrolysis acidifying bacteria to degrade TCE.
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图 1 TCE浓度对累积产酸的影响及比产酸抑制率拟合曲线
注：C*为TCE浓度。
Figure 1. Effects of TCE concentration on cumulative acid production and fitting curve of specific acid production inhibition rate
图 2 TCE浓度对水解酸化菌的SVA的影响
Figure 2. Effect of TCE concentration on SVA of hydrolysis acidifying bacteria
图 3 不同TCE浓度下LB-EPS和TB-EPS的多糖与蛋白质浓度的变化
Figure 3. Variation of polysaccharide and protein concentrations in LB-EPS and TB-EPS under different TCE concentrations
图 4 不同TCE浓度下污泥zeta电位随时间的变化
Figure 4. Variation of sludge zeta potential with time at different TCE concentrations
图 5 水解酸化菌对不同浓度TCE的还原脱氯率
Figure 5. Reduction and dehlorination rates of TCE with different concentrations by hydrolysis acidifying bacteria
图 6 水解酸化反应结束后SMP及EPS的紫外扫描光谱图
Figure 6. UV-vis spectra of SMP and EPS after hydrolysis acidification
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