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三氯乙烯对厌氧水解酸化菌的抑制作用及去除特性

宋雨佩 马玉石 张朝志 沈志强 周岳溪

宋雨佩,马玉石,张朝志,等.三氯乙烯对厌氧水解酸化菌的抑制作用及去除特性[J].环境工程技术学报,2023,13(3):1088-1096 doi: 10.12153/j.issn.1674-991X.20220650
引用本文: 宋雨佩,马玉石,张朝志,等.三氯乙烯对厌氧水解酸化菌的抑制作用及去除特性[J].环境工程技术学报,2023,13(3):1088-1096 doi: 10.12153/j.issn.1674-991X.20220650
SONG Y P,MA Y S,ZHANG C Z,et al.Inhibition and removal characteristics of trichloroethylene on anaerobic hydrolysis acidifying bacteria[J].Journal of Environmental Engineering Technology,2023,13(3):1088-1096 doi: 10.12153/j.issn.1674-991X.20220650
Citation: SONG Y P,MA Y S,ZHANG C Z,et al.Inhibition and removal characteristics of trichloroethylene on anaerobic hydrolysis acidifying bacteria[J].Journal of Environmental Engineering Technology,2023,13(3):1088-1096 doi: 10.12153/j.issn.1674-991X.20220650

三氯乙烯对厌氧水解酸化菌的抑制作用及去除特性

doi: 10.12153/j.issn.1674-991X.20220650
基金项目: 中央财政科技计划结余经费专项(2021-JY-32);国家水体污染控制与治理科技重大专项(2017ZX07402-002)
详细信息
    作者简介:

    宋雨佩(1998—),女,硕士研究生,主要研究方向为水污染控制技术,1273028041@qq.com

    通讯作者:

    周岳溪(1963—),男,研究员,主要研究方向为水污染控制技术,zhouyuexi@263.net

  • 中图分类号: X703

Inhibition and removal characteristics of trichloroethylene on anaerobic hydrolysis acidifying bacteria

  • 摘要:

    三氯乙烯(TCE)是石化废水中典型的有机污染物,对微生物具有极强的毒性。通过对挥发性脂肪酸批次试验进行生物测定,探讨TCE对厌氧水解酸化菌的产酸抑制作用,在TCE作用下水解酸化菌的胞外聚合物(EPS)和污泥zeta电位的变化以及TCE的去除特性。结果表明:TCE浓度为75 mg/L(半抑制浓度,EC50)时,对水解酸化菌的产酸量有抑制作用;随着TCE浓度升高,水解酸化菌的EPS中蛋白质浓度先增大后减少,其中TCE浓度为50 mg/L时EPS中蛋白质浓度达到最大值,为(33.94±0.25)mg/L;zeta电位的结果显示,污泥的凝聚性能随TCE浓度增大(0~100 mg/L)而增大;厌氧水解酸化菌对TCE的脱氯能力随TCE浓度的升高而降低,水解酸化菌转化TCE的脱氯率由TCE浓度为10 mg/L时的77.83%降为200 mg/L时的6.67%。TCE对水解酸化菌具有强烈的抑制作用,TCE主要是通过抑制细胞的蛋白质合成来抑制微生物活性,进而限制水解酸化菌降解TCE的能力。

     

  • 图  1  TCE浓度对累积产酸的影响及比产酸抑制率拟合曲线

    注:C*为TCE浓度。

    Figure  1.  Effects of TCE concentration on cumulative acid production and fitting curve of specific acid production inhibition rate

    图  2  TCE浓度对水解酸化菌的SVA的影响

    Figure  2.  Effect of TCE concentration on SVA of hydrolysis acidifying bacteria

    图  3  不同TCE浓度下LB-EPS和TB-EPS的多糖与蛋白质浓度的变化

    Figure  3.  Variation of polysaccharide and protein concentrations in LB-EPS and TB-EPS under different TCE concentrations

    图  4  不同TCE浓度下污泥zeta电位随时间的变化

    Figure  4.  Variation of sludge zeta potential with time at different TCE concentrations

    图  5  水解酸化菌对不同浓度TCE的还原脱氯率

    Figure  5.  Reduction and dehlorination rates of TCE with different concentrations by hydrolysis acidifying bacteria

    图  6  水解酸化反应结束后SMP及EPS的紫外扫描光谱图

    Figure  6.  UV-vis spectra of SMP and EPS after hydrolysis acidification

    表  1  水解酸化菌的营养物质配比[24]

    Table  1.   Nutrient ratio of hydrolysis acidifying bacteria mg/L 

    物质浓度物质浓度
    NH4HCO32 096MnSO4∙H2O39.2
    K2HPO4500FeSO4∙7H2O100
    MgCl∙6H2O400CuSO4∙5H2O20
    CoCl2∙6H2O0.6NaHCO32 688
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