纳米二氧化钛与磷互作对莱茵衣藻砷累积与生物转化的影响

张鑫; 杨帆; 于子悦; 颜昌宙

doi:10.12153/j.issn.1674-991X.20220728

纳米二氧化钛与磷互作对莱茵衣藻砷累积与生物转化的影响

doi: 10.12153/j.issn.1674-991X.20220728

张鑫^{1, 2,},
杨帆¹,
于子悦^{1, 2},
颜昌宙^1, ,

1.
中国科学院城市环境研究所城市环境与健康重点实验室
2.
中国科学院大学

基金项目: 国家自然科学基金项目（21906157）

详细信息

作者简介:
张鑫（1997—），女，硕士研究生，主要从事水环境与水生态研究，xinzhang@iue.ac.cn

通讯作者:
颜昌宙（1969—），男，研究员，博士，主要从事污染物环境效应与生态风险研究，czyan@iue.ac.cn

中图分类号: X173
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- 被引次数: 0
出版历程
- 收稿日期: 2022-07-19
- 网络出版日期: 2023-07-19

The interactive effects of titanium dioxide nanoparticles and phosphate on arsenic accumulation and biotransformation in Chlamydomonas reinhardtii

ZHANG Xin^{1, 2
,},
YANG Fan¹,
YU Ziyue^{1, 2},
YAN Changzhou^{1
, ,}

1.
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences
2.
University of Chinese Academy of Sciences

摘要

摘要:
纳米材料因其较大的比表面积以及较强的反应活性，对砷（As）的环境行为具有一定的调控作用，而这可能对微藻As吸收代谢产生潜在的影响。以模式生物莱茵衣藻（Chlamydomonas reinhardtii）为研究对象，探究不同磷酸盐（PO₄ ³⁻）浓度下，纳米二氧化钛（nano-TiO₂）对莱茵衣藻中As(Ⅴ)累积和生物转化的影响。结果表明：暴露初期（第1天）nano-TiO₂作为载体显著促进了0.013、0.100和0.500 mmol/L PO₄ ³⁻处理组藻细胞对As的累积，但随着暴露时间的延长，nano-TiO₂的载体效应呈下降趋势；暴露结束后（第8天），nano-TiO₂添加组中，进入藻细胞的As(Ⅴ)除了还原成As(Ⅲ)及甲基化成二甲基砷外，还能进一步转化为一种可能为砷糖的未知化合物，且随着PO₄ ³⁻浓度的降低，藻细胞内这种砷糖所占比例逐渐增加，这可能会抑制As(Ⅲ)的外排；暴露结束后（第8天），培养基中主要检测到的As形态为As(Ⅴ)和As(Ⅲ)，1.0和0.5 mmol/L处理组还有少量二甲基砷。nano-TiO₂的添加降低了培养基中As(Ⅲ)的浓度，尤其是0.5和1.0 mmol/L PO₄ ³⁻处理组。研究结果表明，纳米材料与PO₄ ³⁻的互作可显著改变微藻As的累积与代谢过程。
- 莱茵衣藻 /
- 砷酸盐 /
- 磷酸盐 /
- 纳米二氧化钛 /
- 砷形态
Abstract:
Nanomaterials can modify the environmental behavior of arsenic (As) due to their large specific surface area and high reaction activity, which may affect the absorption and metabolism of As in microalgae. In this study, Chlamydomonas reinhardtii was used as model organism to investigate the influence of titanium dioxide nanoparticles (nano-TiO₂) on As(Ⅴ) accumulation and biotransformation in algal cells at different phosphate concentrations. The results showed that nano-TiO₂ significantly promoted As accumulation in algae cells in 0.013, 0.100 and 0.500 mmol/L phosphate groups at the beginning of exposure (1 d), but the carrier effect of nano-TiO₂ decreased with the extension of exposure time. After 8 days of exposure, in the nano-TiO₂ addition groups, As(Ⅴ) in algal cells was not only reduced to As(Ⅲ) and methylated to dimethyl arsenic, but also further transformed to an unknown As compound, possibly arsenosugars. And the proportion of arsenosugars in algal cells gradually increased with the decrease of phosphate concentration, which might inhibit the efflux of As(Ⅲ). After 8 days of exposure, As(Ⅴ) and As(Ⅲ) were the main As species in the culture medium, and a small amount of dimethyl arsenic was also detected. The addition of nano-TiO₂ decreased the proportions of As(Ⅲ) in the culture medium, especially in 0.5 and 1.0 mmol/L phosphate group. This study indicated that the interaction between nanomaterials and phosphate significantly affected the accumulation and metabolism of As in microalgae, which facilitates the application of microalgae in As remediation.
- Chlamydomonas reinhardtii /
- arsenate /
- phosphate /
- nano-TiO₂ /
- As species

HTML全文

图 1 nano-TiO₂在TAP培养基中的保持率

Figure 1. Retention rate of nano-TiO₂ in TAP medium

下载: 全尺寸图片幻灯片

图 2 nano-TiO₂在TAP培养基中对As(Ⅴ)的吸附率及吸附量

Figure 2. Adsorption rate and adsorption capacity of As(Ⅴ) of nano-TiO₂ in TAP medium

下载: 全尺寸图片幻灯片

图 3 不同PO₄^3-及nano-TiO₂浓度下莱茵衣藻的藻密度变化

Figure 3. Density changes of Chlamydomonas reinhardtii under different concentrations of PO₄ ³⁻ and nano-TiO₂

下载: 全尺寸图片幻灯片

图 4 不同处理下莱茵衣藻细胞不同生长阶段对As的累积

注：不同字母代表同一时间点组间差异显著。

Figure 4. As accumulation in Chlamydomonas reinhardtii cells at different growth stages in different treatments

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图 5 不同处理下莱茵衣藻细胞中各As形态占比（第8天）

Figure 5. Percentage of As speciation in Chlamydomonas reinhardtii cells in different treatments (Day 8)

下载: 全尺寸图片幻灯片

图 6 不同形态As的HPLC-ICP-MS谱图

Figure 6. HPLC-ICP-MS spectra of As species

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图 7 不同处理下培养基中各As形态占比（第8天）

Figure 7. Percentage of As species in culture medium of different treatments (Day 8)

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表 1 试验分组设计

Table 1. Experimental group design

试验组别	As(Ⅴ)浓度/(μmol/L)	PO₄ ³⁻浓度/(mmol/L)	nano-TiO₂浓度/(mg/L)
P_0.013T₀	10	0.013	0
P_0.013T₂	10	0.013	2
P_0.013T₂₀	10	0.013	20
P_0.1T₀	10	0.1	0
P_0.1T₂	10	0.1	2
P_0.1T₂₀	10	0.1	20
P_0.5T₀	10	0.5	0
P_0.5T₂	10	0.5	2
P_0.5T₂₀	10	0.5	20
P_1.0T₀	10	1.0	0
P_1.0T₂	10	1.0	2
P_1.0T₂₀	10	1.0	20

下载: 导出CSV

表 2 不同条件下的吸附动力学参数

Table 2. Adsorption kinetic parameters under different conditions

试验组别	准一级动力学		准二级动力学
试验组别	k₁/〔g/(mg·min)〕	R²	k₂/〔g/(mg·min)〕	R²
P_0.013T₂	0.26	0.95	0.05	0.98
P_0.1T₂	0.28	0.98	0.10	1.00
P_0.5T₂	0.20	0.92	0.07	0.98
P_1.0T₂	0.30	0.87	0.15	0.94
P_0.013T₂₀	0.13	0.94	0.08	0.97
P_0.1T₂₀	0.13	0.92	0.08	0.95
P_0.5T₂₀	0.76	0.92	0.38	0.97
P_1.0T₂₀	0.56	0.95	0.36	0.98

下载: 导出CSV

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