Advanced nitrogen removal efficiency of MSW leachate treated by SND with ASBR combined with SBBR process
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摘要:
为了提高垃圾渗滤液生化处理的TN去除率，采用厌氧序批式反应器（ASBR）串联序批式生物膜反应器（SBBR）处理化学需氧量（COD）为（5 700±500）mg/L、TN浓度为（210±50）mg/L的实际垃圾渗滤液。结果表明：ASBR的出水进入SBBR反应器进行深度脱氮，主要作用是调节后续SBBR进水的碳氮比（C/N），ASBR对渗滤液COD的去除率为90%。C/N是决定SBBR脱氮效率的关键，进水C/N调至4.8，在生物膜的作用下，SBBR仅通过厌氧搅拌和好氧阶段的同步硝化反硝化（SND）便可以实现对垃圾渗滤液的深度脱氮，出水TN浓度低于10 mg/L，周期运行时长也由第54天的24 h缩短至5.6 h。整个串联系统经过103 d的驯化和启动可以达到最佳的处理效果，出水COD、氨氮（NH4 +-N）、TN浓度分别为（380±10）、（1.0±0.5）、（5±5）mg/L，去除率分别达到93%、99%和95%。通过高通量测序分析可知，系统中变形菌门（Proteobacteria）和拟杆菌门（Bacteroidetes）相对丰度较高，分别为55.11%、21.32%。系统中具有反硝化作用的厚壁菌门（Firmicutes）相对丰度占比为2.81%，这可能是SBBR取得优秀脱氮效果的关键。在属水平下，系统中具有反硝化功能的菌种主要为Thauera和Limnobacter，在系统中占比分别为15.22%和2.84%，它们的存在可能是系统SND效果好的主要原因之一。
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关键词:
- 垃圾渗滤液 /
- 序批式生物膜反应器(SBBR) /
- 碳氮比(C/N) /
- 深度脱氮 /
- 同步硝化反硝化(SND) /
- 微生物群落结构
Abstract:In order to improve TN removal rate of leachate, the anaerobic sequencing batch reactor (ASBR) combined with the improved sequencing batch biofilm reactor (SBBR) process was used to treat the actual municipal solid waste (MSW) leachate with chemical oxygen demand (COD) of (5 700±500) mg/L and TN concentration of (210±50) mg/L. The results showed that the effluent of ASBR entered SBBR reactor for deep denitrification, and the main function was to adjust C/N of subsequent SBBR influent, and the removal rate of COD in leachate was 90%. C/N was the key to determining the nitrogen removal efficiency of SBBR. After adjusting the influent C/N to 4.8, under the action of biofilm, the deep denitrification of MSW leachate could be achieved only by anaerobic stirring and simultaneous nitrification and denitrification (SND) in the aerobic stage. The effluent total nitrogen concentration was lower than 10 mg/L, and the cycle running time was shortened from 24 hours on the 54th day to 5.6 hours. The best treatment effect could be achieved after 103 days of domestication and start-up of the whole system. The concentrations of COD, NH4 +-N and TN in the effluent were (380±10), (1.0±0.5) and (5±5) mg/L, respectively, and the removal rates were more than 93%, 99% and 95%, respectively. Through high-throughput sequencing analysis, the relative abundance of Proteobacteria and Bacteroides in the system was high, with 55.11% and 21.32%, respectively. In the system, the abundance of Firmicutes with denitrification accounted for 2.81%, which may be the key to achieving an excellent denitrification effect in SBBR. At the genus level, the bacteria with denitrification function in the system were mainly Thauera and Limnobacter, accounting for 15.22% and 2.84%, respectively. Their existence may be one of the main reasons for the good effect of the system SND. The system achieved deep denitrification of leachate without adding any carbon source and had high nitrogen removal efficiency, which provided an effective way for MSW leachate to meet the discharge standard of total nitrogen.
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图 4 SBBR对渗滤液中不同形态氮的去除
Figure 4. Various forms of nitrogen removal performance in leachate by SBBR
图 5 SBBR运行过程中反硝化+SND和内源反硝化脱氮量的变化
Figure 5. Denitrification amount by Denitrification+SND and endogenous denitrification during SBBR operation
图 6 运行过程中TN浓度和TN去除率的变化
Figure 6. Changes of TN concentration and TN removal rate during operation
图 7 SBBR中门水平上的细菌物种相对丰度
Figure 7. Relative abundance of bacterial species at phylum level within SBBR
图 8 SBBR中属水平上相对丰度排名前10的细菌物种分类情况
注：不同颜色的圆圈表示不同的分类水平，圆圈的大小代表该分类的相对丰度。分类名旁的2个数字均表示相对丰度占比，前者表示该分类在样本所有物种中的占比，后者表示该分类在样本所选取的10种物种中的占比。
Figure 8. Classification of bacterial species with relative abundance ranking top 10 at genus level within SBBR
表 1 试验用垃圾渗滤液主要水质指标
Table 1. Characteristics of the leachate from MSW transfer station
mg/L COD BOD5 NH4 +-N浓度 TN浓度 NOx −-N浓度 5 200~6 200 2 560~2 800 190~210 200~220 0.5~2.0 
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表 2 ASBR-SBBR串联系统对渗滤液COD、NH4 +-N和TN的处理效果
Table 2. Treatment effect of ASBR+SBBR system on COD, ammonia nitrogen and total nitrogen of MSW leachate
项目 COD NH4 +-N TN 数值/
（mg/L）去除率/% 浓度/
（mg/L）去除率/% 浓度/
（mg/L）去除率/% 进水 5 700 210 220 出水 <380 >93 <1 >99 <10 >95
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表 3 SBBR中活性污泥丰富度和多样性指数
Table 3. Richness and diversity indexes of activated sludge of SBBR
样品
名称观测到的
物种数多样性指数 Shannon Simpson Chao1 ACE 覆盖率 SBBR 1 088 5.603 0.965 1 041.420 1 076.212 0.996
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[1] TALALAJ I A, BEIDKA P, BARTKOWSKA I. Treatment of landfill leachates with biological pretreatments and reverse osmosis[J]. Environmental Chemistry Letters, 2019, 17:1177-1193 [2] ZHANG F Z, PENG Y Z, WANG S Y, et al. Efficient step-feed partial nitrification, simultaneous Anammox and denitrification (SPNAD) equipped with real-time control parameters treating raw mature landfill leachate[J]. Journal of Hazardous Materials,2019,364:163-172. doi: 10.1016/j.jhazmat.2018.09.066 [3] 李钦钦, 赵坤.垃圾转运站渗滤液处理技术研究进展[J]. 辽宁化工,2022,51(10):1466-1469. doi: 10.14029/j.cnki.issn1004-0935.2022.10.035LI Q Q, ZHAO K. Research progress of the treatment technology of leachate from waste transfer station[J]. Liaoning Chemical Industry,2022,51(10):1466-1469. doi: 10.14029/j.cnki.issn1004-0935.2022.10.035 [4] 代晋国, 宋乾武, 张玥, 等.新标准下我国垃圾渗滤液处理技术的发展方向[J]. 环境工程技术学报,2011,1(3):270-274. doi: 10.3969/j.issn.1674-991X.2011.03.045DAI J G, SONG Q W, ZHANG Y, et al. Directions for development of landfill leachate treatment technologies under the new standard in China[J]. Journal of Environmental Engineering Technology,2011,1(3):270-274. doi: 10.3969/j.issn.1674-991X.2011.03.045 [5] CHEONG D Y, HANSEN C L. Effect of feeding strategy on the stability of anaerobic sequencing batch reactor responses to organic loading conditions[J]. Bioresource Technology,2008,99(11):5058-5068. doi: 10.1016/j.biortech.2007.08.084 [6] OLIVEIRA R P, RATUSZNEI S M, RODRIGUES J A D, et al. Interaction effects of organic load and cycle time in an AsBr applied to a personal care industry wastewater treatment[J]. Journal of Environmental Management,2010,91(12):2499-2504. doi: 10.1016/j.jenvman.2010.07.015 [7] 吴莉娜, 史枭, 张杰, 等.UASB1-A/O-UASB2深度处理垃圾渗滤液[J]. 环境科学研究,2015,28(8):1331-1336. doi: 10.13198/j.issn.1001-6929.2015.08.22WU L N, SHI X, ZHANG J, et al. Advanced treatment of landfill leachate using the UASB1-A/O-UASB2[J]. Research of Environmental Sciences,2015,28(8):1331-1336. doi: 10.13198/j.issn.1001-6929.2015.08.22 [8] LUO Z Y, CHEN W M, WEN P, et al. Impact of leachate recirculation frequency on the conversion of carbon and nitrogen in a semi-aerobic bioreactor landfill[J]. Environmental Science and Pollution Research,2019,26(13):13354-13365. doi: 10.1007/s11356-019-04817-8 [9] 王茜, 陈琴, 曾涛涛, 等.基于短程硝化工艺的垃圾渗滤液脱氮处理研究进展[J]. 环境工程技术学报,2016,6(2):127-132. doi: 10.3969/j.issn.1674-991X.2016.02.019WANG X, CHEN Q, ZENG T T, et al. Review of nitrogen removal for landfill leachate based on partial nitrification technology[J]. Journal of Environmental Engineering Technology,2016,6(2):127-132. doi: 10.3969/j.issn.1674-991X.2016.02.019 [10] TSILOGEORGIS J, ZOUBOULIS A, SAMARAS P, et al. Application of a membrane sequencing batch reactor for landfill leachate treatment[J]. Desalination,2008,221(1/2/3):483-493. [11] TAKABATAKE H, SATOH H, MINO T, et al. Recovery of biodegradable plastics from activated sludge process[J]. Water Science and Technology,2000,42(3/4):351-356. [12] WANG K, WANG S Y, ZHU R L, et al. Advanced nitrogen removal from landfill leachate without addition of external carbon using a novel system coupling ASBR and modified SBR[J]. Bioresource Technology,2013,134:212-218. doi: 10.1016/j.biortech.2013.02.017 [13] CHIU Y C, LEE L L, CHANG C N, et al. Control of carbon and ammonium ratio for simultaneous nitrification and denitrification in a sequencing batch bioreactor[J]. International Biodeterioration & Biodegradation,2007,59(1):1-7. [14] YIN J, ZHANG P Y, LI F, et al. Simultaneous biological nitrogen and phosphorus removal with a sequencing batch reactor-biofilm system[J]. International Biodeterioration & Biodegradation,2015,103:221-226. [15] THIRD K A, BURNETT N, CORD-RUWISCH R. Simultaneous nitrification and denitrification using stored substrate (PHB) as the electron donor in an SBR[J]. Biotechnology and Bioengineering,2003,83(6):706-720. doi: 10.1002/bit.10708 [16] JIANG J F, MA L A, HAO L J, et al. Comparative study on advanced nitrogen removal of landfill leachate treated by SBR and SBBR[J]. Water,2021,13(22):3240. doi: 10.3390/w13223240 [17] 国家环境保护总局. 水和废水监测分析方法[M]. 4版. 北京: 中国环境科学出版社, 2002. [18] KRISHNA C, van LOOSDRECHT M C M. Effect of temperature on storage polymers and settleability of activated sludge[J]. Water Research,1999,33(10):2374-2382. doi: 10.1016/S0043-1354(98)00445-X [19] 冯旭东, 王斌, 潘登, 等.垃圾渗滤液SND生物脱氮的工程实践[J]. 环境工程,2004,22(6):7-10. doi: 10.3969/j.issn.1000-8942.2004.06.001FENG X D, WANG B, PAN D, et al. Application of simultaneous nitrification and denitrification in landfill leachate treament[J]. Environmental Engineering,2004,22(6):7-10. doi: 10.3969/j.issn.1000-8942.2004.06.001 [20] 张绍青, 张立秋, 张朝升, 等.SBR反应器短程同步硝化反硝化处理垃圾渗滤液[J]. 中国给水排水,2014,30(3):10-13. doi: 10.19853/j.zgjsps.1000-4602.2014.03.003ZHANG S Q, ZHANG L Q, ZHANG C S, et al. Simultaneous nitrification and denitrification via nitrite for treatment of landfill leachate in sequencing batch reactor[J]. China Water & Wastewater,2014,30(3):10-13. doi: 10.19853/j.zgjsps.1000-4602.2014.03.003 [21] 魏桃员, 陈玉婷, 何培弘, 等.SBR工艺处理早期垃圾渗滤液的试验研究[J]. 环境科学与技术,2016,39(4):107-113.WEI T Y, CHEN Y T, HE P H, et al. Experimental treatment of initial landfill leachate by SBR system[J]. Environmental Science & Technology,2016,39(4):107-113. [22] WANG X H, ZHU M H, LI F F, et al. Long-term effects of multi-walled carbon nanotubes on the performance and microbial community structures of an anaerobic granular sludge system[J]. Applied Microbiology and Biotechnology,2018,102(21):9351-9361. doi: 10.1007/s00253-018-9273-1 [23] GAO H, MAO Y P, ZHAO X T, et al. Genome-centric metagenomics resolves microbial diversity and prevalent truncated denitrification pathways in a denitrifying PAO-enriched bioprocess[J]. Water Research,2019,155:275-287. doi: 10.1016/j.watres.2019.02.020 [24] YANG Z C, ZHOU Q, SUN H M, et al. Metagenomic analyses of microbial structure and metabolic pathway in solid-phase denitrification systems for advanced nitrogen removal of wastewater treatment plant effluent: a pilot-scale study[J]. Water Research,2021,196:117067. doi: 10.1016/j.watres.2021.117067 [25] CHEN X Z, WANG X J, ZHONG Z, et al. Biological nitrogen removal via combined processes of denitrification, highly efficient partial nitritation and Anammox from mature landfill leachate[J]. Environmental Science and Pollution Research,2020,27(23):29408-29421. doi: 10.1007/s11356-020-09185-2 [26] SONG J Y, ZHANG W, GAO J F, et al. A pilot-scale study on the treatment of landfill leachate by a composite biological system under low dissolved oxygen conditions: performance and microbial community[J]. Bioresource Technology,2020,296:122344. doi: 10.1016/j.biortech.2019.122344 [27] DÍEZ-VIVES C, GASOL J M, ACINAS S G. Evaluation of marine bacteroidetes-specific primers for microbial diversity and dynamics studies[J]. Microbial Ecology,2012,64(4):1047-1055. doi: 10.1007/s00248-012-0087-x [28] McILROY S J, KARST S M, NIERYCHLO M, et al. Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge[J]. The ISME Journal,2016,10(9):2223-2234. doi: 10.1038/ismej.2016.14 [29] YAN H H, HAN L, YIN Q, et al. Corn starch processing wastewater treated by a full-scale expanded granular sludge bed reactor and comprehensive analysis of microbial community at low and high organic loading rate[J]. IOP Conference Series:Earth and Environmental Science,2021,776(1):012003. doi: 10.1088/1755-1315/776/1/012003 [30] ANTWI P, ZHANG D C, SU H, et al. Nitrogen removal from landfill leachate by single-stage anammox and partial-nitritation process: effects of microaerobic condition on performance and microbial activities[J]. Journal of Water Process Engineering,2020,38:101572. doi: 10.1016/j.jwpe.2020.101572 [31] YUAN J, LAI Q, ZHENG T, et al. Novosphingobium indicum sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from a deep-sea environment[J]. International Journal of Systematic and Evolutionary Microbiology,2009,59(8):2084-2088. doi: 10.1099/ijs.0.002873-0 [32] LIM J H, BAEK S H, LEE S T. Ferruginibacter alkalilentus gen. nov., sp. nov. and Ferruginibacter lapsinanis sp. nov., novel members of the family ‘Chitinophagaceae’ in the Phylum Bacteroidetes, isolated from freshwater sediment[J]. International Journal of Systematic and Evolutionary Microbiology,2009,59(10):2394-2399. doi: 10.1099/ijs.0.009480-0 [33] LIANG Y H, LI D, ZENG H P, et al. Rapid start-up and microbial characteristics of partial nitrification granular sludge treating domestic sewage at room temperature[J]. Bioresource Technology,2015,196:741-745. doi: 10.1016/j.biortech.2015.08.003 [34] GABARRÓ J, HERNÁNDEZ-DEL AMO E, GICH F, et al. Nitrous oxide reduction genetic potential from the microbial community of an intermittently aerated partial nitritation SBR treating mature landfill leachate[J]. Water Research,2013,47(19):7066-7077. ⊗ doi: 10.1016/j.watres.2013.07.057 -
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