安徽某焦化污染场地生物泥浆反应器中试研究案例

李霏; 丁浩然; 杨乐巍; 刘渊文; 王恒; 张岳; 李书鹏; 陈成; 田德金

doi:10.12153/j.issn.1674-991X.20230145

安徽某焦化污染场地生物泥浆反应器中试研究案例

doi: 10.12153/j.issn.1674-991X.20230145

李霏^{1, 2,},
丁浩然¹,
杨乐巍^{1, 2, ,},
刘渊文^{1, 2},
王恒³,
张岳¹,
李书鹏^{1, 2},
陈成¹,
田德金¹

1.
北京建工环境修复股份有限公司
2.
污染场地安全修复技术国家工程实验室
3.
合肥东部新中心建设管理办公室

基金项目: 国家重点研发计划项目（2020YFC1807905）

详细信息

作者简介:
李霏（1989—)，男，博士，主要从事环境微生物方面的研究，lifei@bceer.com

通讯作者:
杨乐巍(1972—)，男，正高级工程师，博士，主要从事土壤及地下水修复研究，yangyuewei@bceer.com

中图分类号: X53
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- 文章访问数: 274
- HTML全文浏览量: 119
- PDF下载量: 45
- 被引次数: 0
出版历程
- 收稿日期: 2023-02-24
- 录用日期: 2023-06-12
- 修回日期: 2023-04-10

Field testing of pilot-scale bioslurry reactor for coking contaminated site: a case study in Anhui Province

LI Fei^{1, 2
,},
DING Haoran¹,
YANG Yuewei^{1, 2
, ,},
LIU Yuanwen^{1, 2},
WANG Heng³,
ZHANG Yue¹,
LI Shupeng^{1, 2},
CHEN Cheng¹,
TIAN Dejin¹

1.
Beijing Construction Engineering Group Environmental Remediation Co., Ltd.
2.
National Engineering Laboratory for Site Remediation Technologies
3.
Hefei East New Center Construction Management Office

摘要

摘要:
多环芳烃（PAHs）尤其是高环PAHs作为焦化污染场地的特征污染物，是一种高毒性、难降解的污染物。生物泥浆生物反应器技术由于具有较高的可调控性，且对难溶解有机物的去除效果好，是一种具有工程化前景的土壤修复工艺。采用自主研发的1 m³泥浆反应罐和商业化的PAHs降解菌剂，在代表性污染土壤进行中试试验，探索了接种菌剂后微生物群落结构变化、优化含固率和微生物反应关键参数的波动范围。结果表明：微生物在营养物质的刺激下，第3~6周快速增殖，其中Hydrogenophaga、Sphingomonadaceae、Pseudomonas等菌属增殖明显，可能参与了PAHs的降解；具有代表性的高环PAHs污染物苯并[a]蒽、苯并[b]荧蒽、苯并[a]芘浓度从几倍于GB 36600—2018《土壤环境质量建设用地土壤污染风险管控标准（试行）》一类建设用地的管控目标值降至修复目标值以下。针对国内缺少实际场地中试规模数据的问题，获得可靠的泥浆反应器运行数据，有助于推动泥浆生物反应器技术在国内向工程化规模扩展。
- 多环芳烃（PAHs） /
- 生物降解 /
- 泥浆反应器 /
- 中试 /
- 焦化污染场地
Abstract:
Polycyclic aromatic hydrocarbons (PAHs), especially high-molecular-weight PAHs, are the characteristic pollutants in coking contaminated sites, which are documented as highly toxic and recalcitrant to degrade. Due to the high controllability and high efficiency for insoluble organic pollutants, bioslurry reactor technology is a promising engineering process for soil remediation. To explore the variations of microbial communities after inoculation, and optimize the fluctuation range of solid content and the key parameters of microbial reaction, a self-developed 1 m³ slurry reactor tank combined with commercialized PAHs-degrading agent was adopted to conduct a pilot test at a representative contaminated field. The results showed that the microorganisms grew rapidly at the 3rd to 6th week under the stimulation of nutrients, reflected by the obvious proliferation of genus such as Hydrogenophaga, Sphingomonadaceae, and Pseudomonas, which were likely involved in the degradation of PAHs. Meanwhile, the concentrations of representative high molecular weight PAHs, namely benzo[a]anthracene, benzo[b]fluoranthene and benzo[a]pyrene, were reduced from several times the control target of Class 1 of building land in Soil Environmental Quality Risk Control Standard for Soil contamination of Development Land (Trial) (GB 36600-2018) to below the target value. In view of the lack of actual site pilot scale data in China, this study obtained reliable bioslurry reactor operation data and promoted the technology to engineering scale in China.
- polycyclic aromatic hydrocarbons (PAHs) /
- biodegradation /
- bioslurry reactor /
- pilot scale experiment /
- coking contaminated site

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图 1 菌剂群落结构

Figure 1. Microbial community structure

下载: 全尺寸图片幻灯片

图 2 反应罐中污染物浓度监测

Figure 2. Contaminants monitoring in bioreactor tanks

下载: 全尺寸图片幻灯片

图 3 反应罐中石油烃降解

Figure 3. Degradation of petroleum hydrocarbons in bioreactor tanks

下载: 全尺寸图片幻灯片

图 4 反应罐中微生物多样性监测

Figure 4. Microbial diversity monitoring in bioreactor tanks

下载: 全尺寸图片幻灯片

图 5 反应罐中的重要参数

Figure 5. Key parameters of the reactor tanks

下载: 全尺寸图片幻灯片

表 1 试验土壤污染情况

Table 1. Contamination level of sampling soil mg/kg　

污染物	样品1	样品2	一类建设用地标准
Zn	51.0	45.0	15 017
Pb	29.0	28.0	400
As	14.7	15.0	20
石油烃	408.0	460.0	826
苯并[a]蒽	12.1	15.5	5.5
苯并[b]荧蒽	31.7	40.1	5.5
苯并[k]荧蒽	31.0	39.2	55
苯并[a]芘	20.8	25.9	0.55
二苯并[a,h]蒽	6.7	9.3	0.55
茚并[1,2,3-cd]芘	23.0	27.8	5.5

下载: 导出CSV

表 2 菌剂多样性分析

Table 2. Analysis of microbial diversity

样品	生物多样性指数
样品	ACE	Chao1	Simpson	Shannon
空白对照	309.53	309.38	0.49	2.51
T50	281.03	280.64	0.14	0.78
T100	283.77	285.61	0.11	0.67
T200	284.54	285.88	0.12	0.68

下载: 导出CSV

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