Abstract: The biodegradation of organic pollutants is influenced by microbial activities, and quorum sensing (QS) is a crucial mechanism that regulates physiological activities. During the biodegradation process of organic pollutants, QS affects the synthesis of key enzymes for degradation, the formation of biofilms, the regulation of microbial community structure, etc. Furthermore, directly adding QS signaling molecules or using bacterial agents that produce such molecules can promote QS regulation, thereby enhancing organic pollutants biodegradation rates. However, environmental factors like pH, temperature, quorum quenching bacteria, nanoparticles, etc., can negatively impact QS activities. Currently, research on QS regulation of organic pollutant biodegradation is still in its early stages. This review summarizes the progress of related research, discusses the mechanisms of QS on the biodegradation of organic pollutants, outlines strategies to enhance QS regulation, identifies the main environmental factors affecting QS activities, and provides prospects for its application.