不同配方菌剂对黑臭水体及底泥修复效果差异研究

陈世杰; 汪凯; 谭勇; 刘思韵; 周金艳; 裴昌盈; 黄铭涛; 李柏林

doi:10.12153/j.issn.1674-991X.20230272

不同配方菌剂对黑臭水体及底泥修复效果差异研究

doi: 10.12153/j.issn.1674-991X.20230272

陈世杰^{1, 2,},
汪凯²,
谭勇¹,
刘思韵²,
周金艳²,
裴昌盈²,
黄铭涛²,
李柏林^2, ,

1.
中煤科工重庆设计研究院(集团)有限公司
2.
武汉理工大学资源与环境工程学院

基金项目: 中煤科工重庆设计研究院（集团）有限公司自立科技项目（2022-04-51）；国家自然科学基金项目（42277055）

详细信息

作者简介:
陈世杰（1982—），男，高级工程师，主要从事水污染控制技术研究，249758469@qq.com

通讯作者:
李柏林（1983—），男，副教授，博士，主要从事水污染控制技术研究，bolly1221@whut.edu.cn

中图分类号: X524
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- 被引次数: 0
出版历程
- 收稿日期: 2023-04-06
- 录用日期: 2023-08-04
- 修回日期: 2023-05-22
- 网络出版日期: 2023-10-20

Study on the differences in the remediating effects of different formulations of bacterial agents on black and odorous water bodies and sediments

1.
CCTEG Chongqing Engineering (Group) Co., Ltd.
2.
School of Resources and Environmental Engineering, Wuhan University of Technology

摘要

摘要:
为探究不同菌剂对黑臭水体的修复效果，揭示研究过程中微生物群落变化规律，采用不同配方菌剂对黑臭水体进行修复，探讨不同菌剂对上覆水、底泥和微生物的影响。结果表明：投加菌剂组上覆水pH均略高于试验前；化学-复合微生物菌剂组上覆水DO浓度略低于空白组（6.95 mg/L），且上覆水NH₄ ⁺-N和TP去除率分别达到86.60%和66.07%，底泥酸可挥发性硫和有机质去除率分别达到79.20%和52.71%，去除效果均明显优于其他试验组；高通量测序表明，投加菌剂组底泥中硝化、反硝化细菌和有机质降解类细菌均具有较高相对丰度，且Vicinamibacterales下的某属在化学-复合微生物菌剂组中的相对丰度明显高于其他试验组（1.07%~2.18%）。研究显示化学-复合微生物菌剂对黑臭水体具有良好修复效果。
- 微生物菌剂 /
- 黑臭水体 /
- 底泥修复 /
- 微生物群落结构
Abstract:
In order to explore the remediation effect of different bacterial agents on black and odorous water bodies, and reveal the changing law of microbial communities during the research process, different formulations of bacterial agents were used to restore black and odorous water bodies, and the effects of different bacterial agents on overlying water, sediment and microorganisms were discussed. The results showed that the pH of the overlying water in the bacterial agent group was slightly higher than that before the experiment. DO concentration in the overlying water of the chemical-composite microbial agent group was slightly lower than that of the blank group (6.95 mg/L), and the removal rates of NH₄ ⁺-N and TP in the overlying water reached 86.60% and 66.07%, while the removal rates of volatile sulfur (AVS) and organic matter in the sediment reached 79.20% and 52.71%, respectively. The removal effects were significantly better than other experimental groups. High-throughput sequencing showed that nitrifying bacteria、denitrifying bacteria and organic matter degrading bacteria in the sediment of the microbial inoculum group had high relative abundance, and the relative abundance of a genus under Vicinamibacterales in the chemical-compound microbial inoculum group was significantly higher than that in other experimental groups (1.07%-2.18%). The results of this study indicate that the chemical composite microbial agent has a good remediation effect on black and odorous water bodies.
- microbial agents /
- black and odorous water body /
- sediment remediation /
- microbial community structure

HTML全文

图 1 属水平下市售菌剂微生物种群结构

Figure 1. Microbial population structure of commercially available microbial agents at the genus level

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图 2 不同菌剂对上覆水pH及DO浓度的影响

Figure 2. Effects of different bacterial agents on pH and DO of overlying water

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图 3 不同菌剂对上覆水NH₄ ⁺-N浓度的影响

Figure 3. Effects of different bacterial agents on NH₄ ⁺-N of overlying water

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图 4 不同菌剂对上覆水COD_Cr的影响

Figure 4. Effects of different bacterial agents on COD_Cr of overlying water

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图 5 不同菌剂对上覆水TP浓度的影响

Figure 5. Effects of different bacterial agents on TP of overlying water

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图 6 不同菌剂处理后底泥AVS浓度变化

Figure 6. Changes of AVS content in the sediment after treatment with different bacterial agents

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图 7 不同菌剂处理后底泥有机质浓度变化

Figure 7. Changes of organic matter content in the sediment after treatment with different bacterial agents

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图 8 属水平下不同菌剂处理后底泥微生物种群结构

Figure 8. Microbial population structure in sediments treated with different bacterial agents at the genus level

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表 1 市售菌剂性质

Table 1. List of properties of commercially available microbial agents

分类	菌剂	组成	性状	用途
复合微生物菌剂	菌剂1	复合菌种、营养剂等	黄色固体粉末	用于轻污染水体，提高水体透明度，改善水色，提升水体自净能力
复合微生物菌剂	菌剂2	复合菌种、营养剂等	黄色固体粉末	用于黑臭、富营养化等污染严重水体治理，提高水体自净能力
化学-纯微生物菌剂	菌剂3	枯草芽孢杆菌、有机载体、碳酸钙、蛋白质等	黄色固体粉末，活菌数约 1130亿/g	用于河道、市政、生活等污水的处理
化学-纯微生物菌剂	菌剂4	光合细菌、沸石粉	黄色固体粉末，有效菌含量约100 cfu/g	用于调水净水，增加DO浓度

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表 2 上覆水及底泥污染物指标

Table 2. Pollutant indicators of overlying water and sediment

项目	指标	数值
上覆水	pH	7.68～7.83
	DO/（mg/L）	7.12～7.97
	NH₄ ⁺-N/（mg/L）	1.35～1.81
	COD_Cr/（mg/L）	43.33～69.44
	TP/（mg/L）	0.51～0.59
底泥	AVS/（mg/kg）	553.59～554.87
底泥	有机质/（mg/kg）	9.40～9.53

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表 3 试验组中投加的菌剂种类及数量

Table 3. Type and quantity of microbial agents added in the experimental group

试验组	投加菌剂	投加量/（g/L）
1	无	0
2	自制菌剂	2.30
3	菌剂1	0.10
4	菌剂2	0.10
5	菌剂3	0.20
6	菌剂4	0.50

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