水处理用絮凝剂对发光细菌的联合毒性研究

范雪滢; 许玉洁; 吴欣颖; 徐志锐; 梁嘉慧; 林健辉; 梅承芳

doi:10.12153/j.issn.1674-991X.20230687

水处理用絮凝剂对发光细菌的联合毒性研究

doi: 10.12153/j.issn.1674-991X.20230687

范雪滢^{1, 2, 3, 4,},
许玉洁^{1, 2, 3, 4},
吴欣颖^{1, 2, 3, 4},
徐志锐^{1, 2, 3, 4},
梁嘉慧^{1, 2, 3, 4},
林健辉^{1, 2, 3, 4},
梅承芳^{1, 2, 3, 4, ,}

1.
广东省科学院微生物研究所
2.
广东省微生物分析检测中心
3.
华南应用微生物国家重点实验室
4.
广东省菌种保藏与应用重点实验室

基金项目: 广东省科学院打造综合产业技术创新中心行动资金资助项目(2022GDASZH-2022010105)

详细信息

作者简介:
范雪滢（1990—），女，高级工程师，主要从事环境监测、生态毒理和农业生态研究，fanxy@gddcm.com

通讯作者:
梅承芳（1979—），女，正高级工程师，主要从事生态毒理和环境风险评估研究，meichf@gdim.cn

中图分类号: X826
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- 文章访问数: 110
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- 被引次数: 0
出版历程
- 收稿日期: 2023-09-21
- 录用日期: 2024-01-31
- 修回日期: 2024-01-07

Study on the joint toxicity of flocculants used in water treatment on luminescent bacteria

FAN Xueying^{1, 2, 3, 4
,},
XU Yujie^{1, 2, 3, 4},
WU Xinying^{1, 2, 3, 4},
XU Zhirui^{1, 2, 3, 4},
LIANG Jiahui^{1, 2, 3, 4},
LIN Jianhui^{1, 2, 3, 4},
MEI Chengfang^{1, 2, 3, 4
, ,}

1.
Institute of Microbiology, Guangdong Academy of Sciences
2.
Guangdong Detection Center of Microbiology
3.
State Key Laboratory of Applied Microbiology Southern China
4.
Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application

摘要

摘要:
随着絮凝剂在污水污泥处理过程的广泛使用，其残留在水处理系统中可能存在的单一或联合毒性效应日益受到关注。以聚合硫酸铁（PFS）、聚合氯化铝（PAC）和聚丙烯酰胺（PAM）为研究对象，通过发光细菌单一毒性和联合毒性试验探讨其可能造成的毒性效应。结果表明：1）对于单一毒性效应，PFS、PAC和PAM对发光细菌抑制效应的半数效应浓度（EC₅₀）分别为29.41、71.82和1 072.64 mg/L，毒性效应大小为PFS>PAC>PAM。2）对于二元絮凝剂联合毒性效应，PFS-PAM在毒性比为1∶1混合时具有协同作用，而PAC-PAM在毒性比为1∶3和3∶1混合时均存在拮抗作用。3）采用浓度加和（CA）和独立作用（IA）模型对联合毒性进行预测，PFS-PAM组合实测值与CA模型的预测值相近，而PFS-PAC和PAC-PAM组合实测值与IA模型预测结果相近。4）三维偏差响应面结果显示，试验观测值与CA模型预测值的偏差（dCA）和试验观测值与IA模型预测值的偏差（dIA）会随着絮凝剂浓度变化而变化，CA模型在PFS和PAC浓度较高时拟合程度较好，而对于PAC-PAM体系，IA模型的拟合程度较CA模型好。研究发现，PFS和PAC对发光细菌的毒性效应较强，PFS与PAM混合使用可能会增加毒性效应，对水处理系统中的微生物造成环境风险。
- 聚合硫酸铁(PFS) /
- 聚合氯化铝(PAC) /
- 聚丙烯酰胺(PAM) /
- 发光细菌 /
- 联合毒性
Abstract:
With the widespread application of flocculants in water and sewage sludge treatment, the potential single or joint toxic effects of their residues in treatment systems are increasingly receiving attention. Polymeric iron sulfate (PFS), polymeric aluminum chloride (PAC) and polyacrylamide (PAM) were used as research objects to explore their potential toxic effects through single toxicity and joint toxicity tests using luminescent bacteria. The results showed that: 1) In the single toxicity test, median effective concentration (EC₅₀) value of PFS, PAC and PAM of luminescent bacteria were 29.41, 71.82 and 1 072.64 mg/L, respectively. The single toxicity effect order was PFS>PAC>PAM. 2) In the joint toxicity of binary flocculants test, PFS-PAM had synergistic effect when mixed with the toxicity ratio of 1∶1, while PAC-PAM had antagonistic effect when mixed with the toxicity ratio of 1∶3 and 3∶1. 3) The concentration addition (CA) and independent action (IA) models were used to predict joint toxicity. The results of PFS-PAM were consistent with the predicted values of CA model, while the results of PFS-PAC and PAC-PAM were consistent with the predicted values of IA model. 4) The three-dimensional deviation response surface results showed that the deviation between experimental observations and CA model (dCA) and IA model predictions (dIA) values changed with the mass concentrations of the flocculants. The CA model was consistent with the results of PFS-PAC, when the mass concentrations of PFS and PAC were high, while for the PAC-PAM system, the prediction of IA model was better than that of CA model. The results indicate that PFS and PAC have higher toxic effect on luminescent bacteria, and the combined use of PFS and PAM may increase the toxic effect and pose environmental risks to microorganisms in water treatment systems.
- polymeric ferric sulfate (PFS) /
- polyaluminum chloride (PAC) /
- polyacrylamide (PAM) /
- luminescent bacteria /
- joint toxicity

HTML全文

图 1 二元絮凝剂浓度-效应曲线和CA、IA模型预测曲线

Figure 1. Concentration-response curves of binary flocculants and the prediction curves of CA and IA model

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图 2 二元絮凝剂与CA和IA模型的三维偏差响应面

Figure 2. Three-dimension deviation response surface of CA and IA models for binary flocculants

下载: 全尺寸图片幻灯片

表 1 单一絮凝剂含量对发光细菌的浓度-效应拟合参数和毒性数据

Table 1. Fitting parameters of concentration-effect and toxicity data of single flocculants concentration on luminescent bacteria

絮凝剂	A₁	A₂	e	k	R²	EC₅₀/（mg/L）	95%置信限/（mg/L）
PFS	9.65	100.14	31.51	3.138	0.975 6	29.41	25.59，32.43
PAC	−57.79	98.96	57.68	3.599	0.998 3	71.82	70.72，72.52
PAM	4.06	96.16	1073.47	6.112	0.980 5	1 072.64	1 033.83，1 112.31

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表 2 废水急性毒性分级

Table 2. Acute toxicity classes of wastewater

毒性单位范围	分级
<0.4	无毒
0.4~1	微毒
1~10	中毒
10~100	重毒
>100	剧毒

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表 3 二元絮凝剂含量对发光细菌的浓度-效应拟合参数和毒性数据

Table 3. Fitting parameters of concentration-effect and toxicity data of binary flocculants concentration on luminescent bacteria

絮凝剂组合	毒性比	A₁	A₂	x₀	p	R²	EC₅₀/（mg/L）	95%置信限/（mg/L）
PFS-PAC	1∶1	4.21	101.74	79.10	8.347	0.995 6	77.95	77.17，78.66
	1∶3	2.82	92.20	81.42	8.128	0.991 9	82.54	78.95，85.57
	3∶1	4.14	92.12	51.71	6.263	0.991 5	52.42	50.59，54.34
PFS-PAM	1∶1	15.51	99.54	577.98	4.087	0.990 1	528.98	500.18，553.66
	1∶3	16.03	100.35	901.40	3.778	0.995 9	812.21	785.04，840.49
	3∶1	1.343	102.82	361.42	3.520	0.998 3	353.10	347.46，358.94
PAC-PAM	1∶1	−0.568	83.75	849.26	9.058	0.992 1	888.02	876.64，900.07
	1∶3	−0.620	104.75	1176.02	2.829	0.997 6	1 143.87	1 108.72，1 179.67
	3∶1	0.919	93.92	467.61	6.755	0.996 4	475.37	464.37，485.21

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表 4 不同评价方法的联合作用类型判断标准

Table 4. Criteria for types of joint effects using different evaluation methods

TU	MTI	作用类型
TU<1	MTI>1	协同作用
TU=1	MTI=1	简单相加作用
1<TU<M₀	0<MTI<1	部分相加作用
TU=M₀	MTI=0	独立作用
TU>M₀	MTI<0	拮抗作用

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表 5 二元絮凝剂联合作用评价

Table 5. Evaluation of joint effects on binary flocculants

絮凝剂组合	毒性比	TU法			MTI法
絮凝剂组合	毒性比	TU	M₀	联合作用	MTI	联合作用
PFS-PAC	1∶1	1.5	2.0	部分相加作用	0.38	部分相加作用
	1∶3	1.3	1.3	独立作用	0	独立作用
	3∶1	1.3	1.3	独立作用	0	独立作用
PFS-PAM	1∶1	0.96	2.0	协同作用	1.10	协同作用
	1∶3	1.0	1.3	简单相加作用	1.00	简单相加作用
	3∶1	1.2	1.3	部分相加作用	0.31	部分相加作用
PAC-PAM	1∶1	1.6	2.0	部分相加作用	0.36	部分相加作用
	1∶3	1.4	1.3	拮抗作用	−0.16	拮抗作用
	3∶1	1.5	1.3	拮抗作用	−0.36	拮抗作用

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