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微塑料对土壤微生物群落结构影响及共生网络分析

尚耘旭 古雄杰 余红 郑涵云 李文金 全占军 王菲菲

尚耘旭,古雄杰,余红,等.微塑料对土壤微生物群落结构影响及共生网络分析[J].环境工程技术学报,2024,14(3):719-731 doi: 10.12153/j.issn.1674-991X.20240111
引用本文: 尚耘旭,古雄杰,余红,等.微塑料对土壤微生物群落结构影响及共生网络分析[J].环境工程技术学报,2024,14(3):719-731 doi: 10.12153/j.issn.1674-991X.20240111
SHANG Y X,GU X J,YU H,et al.Effects of microplastics on soil microbial community structure and symbiotic network analysis[J].Journal of Environmental Engineering Technology,2024,14(3):719-731 doi: 10.12153/j.issn.1674-991X.20240111
Citation: SHANG Y X,GU X J,YU H,et al.Effects of microplastics on soil microbial community structure and symbiotic network analysis[J].Journal of Environmental Engineering Technology,2024,14(3):719-731 doi: 10.12153/j.issn.1674-991X.20240111

微塑料对土壤微生物群落结构影响及共生网络分析

doi: 10.12153/j.issn.1674-991X.20240111
基金项目: 国家重点研发计划项目(2020YFC1909502)
详细信息
    作者简介:

    尚耘旭(1997—),女,硕士研究生,主要从事新污染物生态环境风险研究,shangyunxu@foxmail.com

    通讯作者:

    全占军(1979—),男,研究员,主要从事生态学研究,quanzj@craes.org.cn

    王菲菲(1978—),女,研究员,主要从事新污染物毒性效应和环境风险研究,wangff@craes.org.cn

  • 中图分类号: X53

Effects of microplastics on soil microbial community structure and symbiotic network analysis

  • 摘要:

    土壤是微塑料的主要储存库,微塑料进入土壤生态系统后可以形成与周围环境显著不同的“塑料圈”,影响土壤微生物群落结构。通过外源添加微塑料颗粒模拟不同程度的农田土壤微塑料污染,探究微塑料对土壤微生物群落的影响。结果显示,微塑料污染会对土壤中微生物属水平细菌和真菌群落带来极大改变,根际土壤和非根际土壤的微塑料处理高浓度组真菌群落特有属数量显著高于低浓度组(p<0.05),且微塑料污染引起的根际土壤与非根际土壤的细菌群落和真菌群落结构变化均存在较大差异。土壤微生物群落多样性及共生网络分析结果表明,高浓度组微塑料添加显著降低土壤微生物多样性,微塑料对根际土壤微生物的影响大于非根际土壤。研究结果提示土壤真菌群落对微塑料污染的响应程度大于细菌群落,未来土壤微塑料相关研究需要进一步重视根际土壤监测。

     

  • 图  1  共有和独有的土壤细菌群落属水平Venn图

    注:图中数字分别表示细菌属数量(占比)。

    Figure  1.  Genus-level Venn diagram of shared and special soil bacterial communities

    图  2  共有和独有的土壤真菌群落属水平Venn图

    Figure  2.  Genus-level Venn diagram of shared and special soil fungal communities

    图  3  门和属水平的细菌群落结构

    Figure  3.  Bacterial community structure at the phylum and genus level

    图  4  细菌门至属水平差异性分析进化分支图(LEfSe分析)

    Figure  4.  Evolutionary branching diagram for variability analysis of bacterial phylum to genus level (LEfSe analysis)

    图  5  门和目水平的真菌群落结构

    Figure  5.  Fungal community structure at the phylum and order level

    图  6  真菌门至属水平差异性分析进化分支图(LEfSe分析)

    Figure  6.  Evolutionary branching diagram for variability analysis of fungal phylum to genus level (LEfSe analysis)

    图  7  土壤细菌群落α多样性——Chao1指数

    注:不同小写字母表示根际土壤在不同微塑料处理组织之间存在显著差异(p<0.05);不同大写字母表示非根际土壤在不同微塑料处理组之间存在显著差异(p<0.05);***表示根际与非根际土壤存在极显著差异(p<0.001)。全文同。

    Figure  7.  Soil bacterial community alpha diversity: Chao1 index

    图  8  根际和非根际土壤细菌群落PLS-DA分析

    Figure  8.  PLS-DA analysis of bacterial communities in rhizosphere and non-rhizosphere soils

    图  9  土壤真菌群落α多样性——Chao1指数

    注:不同小写字母表示根际土壤在不同微塑料处理组织之间存在显著差异(p<0.05);不同大写字母表示非根际土壤在不同微塑料处理组织之间存在显著差异(p<0.05);“***”表示根际与非根际土壤存在极显著差异(p<0.001)。

    Figure  9.  Soil fungal community alpha diversity: Chao1 index

    图  10  根际和非根际土壤真菌群落PLS-DA分析

    Figure  10.  PLS-DA analysis of fungal communities in rhizosphere and non-rhizosphere soils

    图  11  土壤细菌共生网络

    Figure  11.  Soil bacterial symbiotic network

    图  12  土壤真菌共生网络

    Figure  12.  Soil bacterial symbiotic network

    表  1  土壤细菌群落α多样性——Shannon和Simpson指数

    Table  1.   Soil bacterial community alpha diversity: Shannon and Simpson indexes

    根际土壤 Shannon指数 Simpson指数 非根际土壤 Shannon指数 Simpson指数
    0_R 6.24ab 0.992 6ab 0_B 6.44A 0.993 8AB
    0.5_R 6.04bc 0.990 6b 0.5_B 6.22B 0.992 8C
    1_R 6.38ab 0.994 0a 1_B 6.48A 0.993 5B
    2.5_R 6.47a 0.993 9a 2.5_B 6.50A 0.994 3A
    5_R 6.21ab 0.992 4ab 5_B 6.26B 0.993 5B
    10_R 6.02c 0.989 2b 10_B 6.30B 0.993 2BC
    下载: 导出CSV

    表  2  土壤真菌群落α多样性——Shannon和Simpson指数

    Table  2.   Soil fungal community alpha diversity: Shannon and Simpson indexes

    根际土壤 Shannon指数 Simpson指数 非根际土壤 Shannon指数 Simpson指数
    0_R 2.17c 0.726 3b 0_B 2.49C 0.749 2B
    0.5_R 3.66a 0.911 1a 0.5_B 3.71A 0.935 9A
    1_R 3.22ab 0.856 8a 1_B 3.03B 0.828 0B
    2.5_R 3.09ab 0.878 0a 2.5_B 2.63C 0.807 7B
    5_R 3.28ab 0.907 0a 5_B 3.75A 0.920 6A
    10_R 2.88b 0.794 9ab 10_B 3.61A 0.907 7A
    下载: 导出CSV

    表  3  土壤细菌共生网络拓扑参数

    Table  3.   Soil bacterial symbiotic network topological parameters

    土壤类型 节点 平均加权度 网络直径 网络密度 模块化 平均聚类系数 平均路径长度
    根际土壤 200 2212 22.1 7 0.111 0.406 0.547 2.905
    非根际土壤 200 1648 16.5 6 0.083 0.514 0.504 2.919
    下载: 导出CSV

    表  4  土壤真菌共生网络拓扑参数

    Table  4.   Soil fungal symbiotic network topology parameters

    土壤类型 节点 平均加权度 网络直径 网络密度 模块化 平均聚类系数 平均路径长度
    根际土壤 200 1154 11.5 6 0.063 0.582 0.559 3.032
    非根际土壤 200 1028 10.3 6 0.052 0.682 0.605 3.061
    下载: 导出CSV
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  • 收稿日期:  2024-02-26
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