Effects of biochar addition on different forms of nitrogen in facility agricultural soils under various fertilization regimes
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摘要:
生物炭在设施农业土壤中施用，对土壤氮形态具有显著影响。探明不同施肥处理添加生物炭对设施农业土壤不同形态氮的影响，可为设施农业施用生物炭减排提供科学依据。以设施菜地土壤（褐潮土）为研究对象，设置不施肥（CK）、施用有机肥（M）、化肥（F）、有机无机混施（M+F）4种处理下投入2%和4%（生物炭与土壤干质量比）生物炭处理，采用室内恒温好氧培养-气相色谱测定方法监测土壤N2O释放量，测定土壤中可溶性有机氮（DON）和无机氮（Nmin）的含量，并分析DON、Nmin含量变化及其与土壤N2O释放量变化之间的关系。研究表明，生物炭的施用在不同条件下对土壤N2O的释放速率和累积释放量产生不同影响。在CK和M处理下，生物炭在施用初期（第0~1.5天）显著促进了土壤中N2O的释放，但随后（第2~60天），在CK处理下，生物炭的添加对N2O的释放速率和累积释放量没有产生显著影响。同样地，在M处理下，生物炭的添加也未对N2O的释放速率产生显著作用。然而，在培养结束时，添加4%的生物炭显著提高了土壤中N2O的累积释放量。值得注意的是，在F和M+F的处理中，生物炭的加入在初期阶段（第0~2天）有效地降低了土壤N2O的释放速率，这种降低效果随着生物炭施用量的增加而变得更加显著。在F和M+F处理下，添加生物炭在不同时间段内（第2~25天和第3~14天）显著增加了土壤N2O释放速率，但对该速率的影响在后续阶段并不显著。培养结束后，F处理下，添加2%和4%生物炭的土壤N2O累积释放量分别显著提高78%和90%；M+F处理下，添加2%和4%生物炭的土壤N2O累积释放量分别显著提高80%和67%。相关性分析结果显示，在施用生物炭的土壤中，DON和Nmin的含量与N2O的排放量之间存在明显的正相关关系。表明生物炭的添加通过调整土壤中DON和Nmin的含量，对N2O的排放产生了直接影响。将生物炭投入到不同的施肥土壤中，土壤N2O的释放速率和累积释放量出现不同的变化趋势，但是由于生物炭自身特性的多样性、配施化肥和有机肥种类的差异、施肥方式和时间的差异等，因此分析生物炭添加后对土壤N2O累积释放量影响时，需要根据研究时的具体条件做合理分析。
Abstract:Biochar application in facility agriculture soil has a significant impact on the forms of soil nitrogen. Clarifying the effects of different fertilization treatments with the addition of biochar on various forms of nitrogen in the soil of facility agriculture can provide a scientific basis for emission reduction through the application of biochar in facility agriculture. The dissolved organic nitrogen (DON) and inorganic nitrogen (Nmin) in soil were measured in greenhouse vegetable soil (brown fluvio-aquic soil) after biochar application under different fertilization regimes (no fertilizer (CK), manure (M), chemical fertilizer (F), chemical fertilizer plus manure (M+F), using indoor constant temperature aerobic cultivation and gas chromatography. Incubation was carried out to investigate N2O release and the contents of DON, Nmin after 2% (biochar/dry soil) and 4% biochar application under the different fertilization regimes. The influence of biochar addition on soil N2O releases was studied, and the correlation between the changes in DON and Nmin contents and soil N2O releases was analyzed. The research indicated that the application of biochar had different effects on the release rate and cumulative release of soil N2O under different conditions. Under CK and M patterns, biochar application significantly increased the rate and amount of soil N2O release in the early period (0-1.5 d). During 2-60 d, biochar application had no significant effect on the rate and amount of soil N2O release in CK pattern. Biochar application had no significant effect on the soil N2O release rate, but 4% biochar at the end of the incubation significantly increased the cumulative soil N2O release in M pattern. In F and M+F treatments, biochar application reduced the soil N2O release rate in the early period (0-2 d), with an increasingly apparent effect as the amount of biochar applied increased. In F and M+F treatments, biochar application significantly increased the soil N2O release rate during 2-25 and 3-14 d, respectively, but the effect on the rate was not significant in the subsequent stages. After cultivation, the cumulative soil N2O release in F and M+F treatments with 2% and 4% biochar application were significantly increased by 78% and 90%, 80% and 67%, respectively. The correlation analysis results showed that there was an obvious positive correlation between DON and Nmin contents and N2O emission with biochar application. The addition of biochar had a direct impact on N2O release by adjusting the contents of DON and Nmin in the soil. The release rate and cumulative release of N2O from different fertilized soils showed different trends when biochar was added. However, due to the diversity of biochar properties, the variation in types of chemical and organic fertilizers, and differences in fertilization methods and timing, a reasonable analysis was required based on the specific conditions of the study when assessing the impact of biochar addition on soil N2O cumulative release.
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Key words:
- biochar /
- fertilization pattern /
- N2O /
- dissolved organic nitrogen in soil /
- soil inorganic nitrogen
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图 1 不同施肥处理下土壤中N2O释放速率
Figure 1. Release rates of N2O in soil under different treatments during incubation
图 2 不同施肥处理下土壤中N2O累积释放量
Figure 2. Accumulative release of N2O in soil under different treatments duirng incubation
图 3 不同施肥处理下土壤中DON含量动态变化
Figure 3. Dynamics of DON content in soil in different treatments during incubation
图 4 不同施肥处理下土壤中Nmin含量动态变化
Figure 4. Dynamics of Nmin content in soil under different treatments during incubation
图 5 生物炭添加处理中DON、Nmin含量与N2O累积释放量之间的相关性
注：**表示在P<0.01水平上显著相关。
Figure 5. Correlation between DON, Nmin contents and N2O cumulative release under different treatments with biochar addition
表 1 添加物料的基本性质
Table 1. Basic properties of materials used
物料 有机碳含
量/（g/kg）TN含量/
（g/kg）TP含量/
（g/kg）TK含量/
（g/kg）pH 有机肥 138.1 14.13 6.33 30.88 8.09 生物炭 374.4 2.25 1.9 15.18 9.76 
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表 2 不同处理的肥料以及C、N添加量
Table 2. Amount of materials added and input of C and N in different treatments
处理 鲜土质量/g 商品有机肥
添加量/g尿素添加
量/g生物炭
添加量/gCK 71.43 M 71.43 1.8 F 71.43 0.054 5 M+F 71.43 1.8 0.054 5 B1 71.43 1.2 B2 71.43 2.4 M+B1 71.43 1.8 1.2 M+B2 71.43 1.8 2.4 F+B1 71.43 0.054 5 1.2 F+B2 71.43 0.054 5 2.4 M+F+B1 71.43 1.8 0.054 5 1.2 M+F+B2 71.43 1.8 0.054 5 2.4
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[1] STOCKER T F, QIN D, PLATTNER G. Contribution of working group Ⅰ to the fifth assessment report of the Intergovernmental Panel on Climate Change[C]//Climate Change 2013: the Physical Science Basis. Cambridge: Cambridge University Press, 2013. [2] KROEZE C, MOSIER A, BOUWMAN L. Closing the global N2O budget: a retrospective analysis 1500-1994[J]. Global Biogeochemical Cycles,1999,13(1):1-8. doi: 10.1029/1998GB900020 [3] LOECKE T D, ROBERTSON G P. Soil resource heterogeneity in terms of litter aggregation promotes nitrous oxide fluxes and slows decomposition[J]. Soil Biology and Biochemistry,2009,41(2):228-235. doi: 10.1016/j.soilbio.2008.10.017 [4] 陈温福, 张伟明, 孟军, 等. 生物炭应用技术研究[J]. 中国工程科学,2011,13(2):83-89.CHEN W F, ZHANG W M, MENG J, et al. Researches on biochar application technology[J]. Strategic Study of CAE,2011,13(2):83-89. [5] LEHMANN J, JOSEPH S. Biochar for environmental management: science and technology[M]. London: Earthscan, 2009. [6] 吴伟祥, 孙雪, 董达, 等. 生物质炭土壤环境效应[M]. 北京: 科学出版社, 2015: 90-91. [7] ZHANG D X, PAN G X, WU G, et al. Biochar helps enhance maize productivity and reduce greenhouse gas emissions under balanced fertilization in a rainfed low fertility inceptisol[J]. Chemosphere,2016,142:106-113. doi: 10.1016/j.chemosphere.2015.04.088 [8] 程功, 刘廷玺, 李东方, 等. 生物炭和秸秆还田对干旱区玉米农田土壤温室气体通量的影响[J]. 中国生态农业学报(中英文),2019,27(7):1004-1014.CHENG G, LIU T X, LI D F, et al. Effects of biochar and straw on greenhouse gas fluxes of corn fields in arid regions[J]. Chinese Journal of Eco-Agriculture,2019,27(7):1004-1014. [9] CAYUELA M L, SÁNCHEZ-MONEDERO M A, ROIG A, et al. Biochar and denitrification in soils: when, how much and why does biochar reduce N2O emissions[J]. Scientific Reports,2013,3:1732. doi: 10.1038/srep01732 [10] LIN Y X, DING W X, LIU D Y, et al. Wheat straw-derived biochar amendment stimulated N2O emissions from rice paddy soils by regulating the amoA genes of ammonia-oxidizing bacteria[J]. Soil Biology and Biochemistry,2017,113:89-98. doi: 10.1016/j.soilbio.2017.06.001 [11] NELISSEN V, SAHA B K, RUYSSCHAERT G, et al. Effect of different biochar and fertilizer types on N2O and NO emissions[J]. Soil Biology and Biochemistry,2014,70:244-255. doi: 10.1016/j.soilbio.2013.12.026 [12] GLASER B, LEHMANN J, ZECH W. Ameliorating physical and chemical properties of highly weathered soils in the tropics with charcoal: a review[J]. Biology and Fertility of Soils,2002,35(4):219-230. doi: 10.1007/s00374-002-0466-4 [13] ZIMMERMANN M, BIRD M I, WURSTER C, et al. Rapid degradation of pyrogenic carbon[J]. Global Change Biology,2012,18(11):3306-3316. doi: 10.1111/j.1365-2486.2012.02796.x [14] AMELOOT N, de NEVE S, JEGAJEEVAGAN K, et al. Short-term CO2 and N2O emissions and microbial properties of biochar amended sandy loam soils[J]. Soil Biology and Biochemistry,2013,57:401-410. doi: 10.1016/j.soilbio.2012.10.025 [15] ZIMMERMAN A R, GAO B, AHN M Y. Positive and negative carbon mineralization priming effects among a variety of biochar-amended soils[J]. Soil Biology and Biochemistry,2011,43(6):1169-1179. doi: 10.1016/j.soilbio.2011.02.005 [16] SÁNCHEZ-GARCÍA M, ALBURQUERQUE J A, SÁNCHEZ-MONEDERO M A, et al. Biochar accelerates organic matter degradation and enhances N mineralisation during composting of poultry manure without a relevant impact on gas emissions[J]. Bioresource Technology,2015,192:272-279. doi: 10.1016/j.biortech.2015.05.003 [17] FAHAD S, HUSSAIN S, SAUD S, et al. A combined application of biochar and phosphorus alleviates heat-induced adversities on physiological, agronomical and quality attributes of rice[J]. Plant Physiology and Biochemistry: PPB,2016,103:191-198. doi: 10.1016/j.plaphy.2016.03.001 [18] AGEGNEHU G, BASS A M, NELSON P N, et al. Benefits of biochar, compost and biochar-compost for soil quality, maize yield and greenhouse gas emissions in a tropical agricultural soil[J]. The Science of the Total Environment, 2016, 543(Pt A): 295-306. [19] 邓正昕, 高明, 熊子怡, 等. 有机肥配施生物炭对果园土壤反硝化微生物和酶活性的影响[J]. 环境科学,2023,44(12):6955-6964.DENG Z X, GAO M, XIONG Z Y, et al. Effects of organic fertilizer combined with biochar on denitrifying microorganisms and enzyme activities in orchard soil[J]. Environmental Science,2023,44(12):6955-6964. [20] 于亚军, 朱波, 荆光军. 成都平原土壤-蔬菜系统N2O排放特征[J]. 中国环境科学,2008,28(4):313-318.YU Y J, ZHU B, JING G J. N2O emission from soil-vegetable system and impact factors in Chengdu Plain of Sichuan Basin[J]. China Environmental Science,2008,28(4):313-318. [21] 陈海燕, 李虎, 王立刚, 等. 京郊典型设施蔬菜地N2O排放规律及影响因素研究[J]. 中国土壤与肥料,2012(5):5-10.CHEN H Y, LI H, WANG L G, et al. Characteristics and influencing factors on nitrous oxide emissions from typical greenhouse vegetable fields in Beijing suburbs[J]. Soil and Fertilizer Sciences in China,2012(5):5-10. [22] WANG J Y, XIONG Z Q, YAN X Y. Fertilizer-induced emission factors and background emissions of N2O from vegetable fields in China[J]. Atmospheric Environment,2011,45(38):6923-6929. doi: 10.1016/j.atmosenv.2011.09.045 [23] 仇少君, 彭佩钦, 荣湘民, 等. 淹水培养条件下土壤微生物生物量碳、氮和可溶性有机碳、氮的动态[J]. 应用生态学报,2006,17(11):2052-2058.QIU S J, PENG P Q, RONG X M, et al. Dynamics of soil microbial biomass and dissolved organic carbon and nitrogen under flooded condition[J]. Chinese Journal of Applied Ecology,2006,17(11):2052-2058. [24] 张旭博, 徐明岗, 张文菊, 等. 添加有机物料后红壤CO2释放特征与微生物生物量动态[J]. 中国农业科学,2011,44(24):5013-5020.ZHANG X B, XU M G, ZHANG W J, et al. Characteristics of CO2 emission and microbial biomass dynamics after adding various organic materials in red soil[J]. Scientia Agricultura Sinica,2011,44(24):5013-5020. [25] 何莉莉, 黄佳佳, 王梦洁, 等. 生物炭配施硝化抑制剂降低稻田土壤NH3和N2O排放的微生物机制[J]. 植物营养与肥料学报,2023,29(11):2030-2041.HE L L, HUANG J J, WANG M J, et al. Effects of biochar combined with nitrification inhibitor (DMPP) on reducing NH3 and N2O emission in paddy soil and its microbial mechanism[J]. Journal of Plant Nutrition and Fertilizers,2023,29(11):2030-2041. [26] GOLDBERG E. Black carbon in the environment: properties and distribution[M]. New York: John Wiley, 1985. [27] HAMER U, MARSCHNER B, BRODOWSKI S, et al. Interactive priming of black carbon and glucose mineralisation[J]. Organic Geochemistry,2004,35(7):823-830. doi: 10.1016/j.orggeochem.2004.03.003 [28] LUO Y, DURENKAMP M, de NOBILI M, et al. Short term soil priming effects and the mineralisation of biochar following its incorporation to soils of different pH[J]. Soil Biology and Biochemistry,2011,43(11):2304-2314. doi: 10.1016/j.soilbio.2011.07.020 [29] YIN Y F, HE X H, GAO R, et al. Effects of rice straw and its biochar addition on soil labile carbon and soil organic carbon[J]. Journal of Integrative Agriculture,2014,13(3):491-498. doi: 10.1016/S2095-3119(13)60704-2 [30] CHENG C H, LEHMANN J. Ageing of black carbon along a temperature gradient[J]. Chemosphere,2009,75(8):1021-1027. doi: 10.1016/j.chemosphere.2009.01.045 [31] ZIMMERMAN A R. Abiotic and microbial oxidation of laboratory-produced black carbon (biochar)[J]. Environmental Science & Technology,2010,44(4):1295-1301. [32] BAGREEV A, BASHKOVA S, LOCKE D C, et al. Sewage sludge-derived materials as efficient adsorbents for removal of hydrogen sulfide[J]. Environmental Science & Technology,2001,35(7):1537-1543. [33] LIU Y T, LI Y E, WAN Y F, et al. Nitrous oxide emissions from irrigated and fertilized spring maize in semi-arid Northern China[J]. Agriculture, Ecosystems & Environment, 2011, 141(3/4): 287-295. [34] LI Y T, LI Y, YUE L, et al. Simulating N2O emissions from maize-cropped soil and the impact of climatic variations and cropland management in North China Plain[C]//Proceedings of the 19th World Congress of Soil Science: soil solutions for a changing world. Brisbane, 2010. [35] LEHMANN J, PEREIRA Da SILVA J, STEINER C, et al. Nutrient availability and leaching in an archaeological Anthrosol and a Ferralsol of the Central Amazon Basin: fertilizer, manure and charcoal amendments[J]. Plant and Soil,2003,249(2):343-357. doi: 10.1023/A:1022833116184 [36] ZHANG Q Z, DIJKSTRA F A, LIU X R, et al. Effects of biochar on soil microbial biomass after four years of consecutive application in the North China Plain[J]. PLoS One,2014,9(7):e102062. □ doi: 10.1371/journal.pone.0102062 -
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