The mixer of fosfomycin degrading bacteria was added into cyclic activated sludge system (CASS) reactor to treat the effluent from an anaerobic baffled reactor which was fed with pharmaceutical wastewater. The CODCr
-N and TP removal efficiencies were studied, and the comparison between CASS reactor with and without inoculation was carried out. The results showed that when the reactor C2 was inoculated with fosfomycin degrading bacteria, and with the average CODCr
of 1 101.4 mg/L，average NH4+
-N of 134.7 mg/L, average TP of 22.1 mg/L in the influent, the CODCr
-N and TP of effluent were 203.7, 12.6 and 3.2 mg/L, respectively. Accordingly, the average removal efficiency of CODCr
-N and TP was 81.57%, 90.67% and 85.44%, respectively. Comparatively, the average removal efficiency of CODCr
-N, TP of the reactor C1 that was not inoculated with fosfomycin degrading bacteria was 66.91%, 84.30% and 67.41%. Thus the reactor C2 inoculated with fosfomycin degrading bacteria showed better CODCr
-N, TP removal efficiencies than reactor C1. The main fluorescent materials in the influent were identified as aromatic protein substances and soluble microbial products based on the three-dimensional fluorescence spectra analysis. They were removed obviously after inoculated with fosfomycin degrading bacteria.