Abstract: Moderate oxidation drying(MOD)technology was used to treat cephalosporin and penicillin residue harmlessly, and after treatment the antibiotic residues in the samples were below the detection limit(1 mg/kg). After adjusting and treating the residue fertilizer, the targets of organic matter and nitrogen, phosphorus and potassium nutrients and the content of heavy metals all met the requirements of Organic Fertilizer Standard(NY 525-2012), and none of seven kinds of typical drug-resistant genes in antibiotic residues was detected. The further fertilization tests showed that the antibiotic residues after MOD treatment were applied to the soil at 1% and 3%, and no antibiotic residue was detected in the soil after 30 days. The treated residues could reduce the accumulation of drug-resistance genes after application to the soil.